Return to search results Title: Transcriptome analysis of Wnt knockdown embryos reveals candidate canonical Wnt/β-catenin target genes during Xenopus laevis gastrulation

Summary

The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach.

Contributors: Richard Harland, Rachel Kjolby, Richard Harland

Experiment Type: Total RNA was extracted from a single uninjected control and a single dkk injected embryo from the same mating and used to make sequencing libraries. There are three replicates, each pair from a different mating pair.

Experiment Reagents: dkk1.L.

Article: XB-ART-52028, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
WE - NF11.5 GSM2050744  GSM2050746  GSM2050748  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 11.5      WE + dkk1 - NF11.5 DEG GSM2050745  GSM2050747  GSM2050749  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection dkk1.L dkk1.L NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 11.5

Select All DEG

Select All  Xtr    Xla

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