Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleic Acids Res
2000 May 15;2810:2099-107. doi: 10.1093/nar/28.10.2099.
Show Gene links
Show Anatomy links
Bi-directional replication and random termination.
Santamaría D, Viguera E, Martínez-Robles ML, Hyrien O, Hernández P, Krimer DB, Schvartzman JB.
???displayArticle.abstract???
Two-dimensional (2D) agarose gel electrophoresis was used to study termination of DNA replication in a shuttle vector, YRp7', when it replicated in Escherichia coli, Saccharomyces cerevisiae and Xenopus egg extracts. In E. coli, the 2D gel patterns obtained were consistent with uni-directional replication initiated at a specific site, the ColE1 origin. In consequence, termination also occurred precisely at the ColE1 origin. In Xenopus egg extracts, the particular shape of the bubble arc as well as the triangular smear detected to the left of the simple-Y pattern indicated random initiation and termination. In S.cerevisiae, initiation occurred at the ARS1 origin and replication proceeded in a bi-directional manner. However, termination did not always occur at a specific site 180 degrees across from the origin, but almost all along the south hemisphere of the plasmid. Inversion, deletion or replacement of DNA sequences located throughout this hemisphere did not eliminate random termination. Analysis of the replication intermediates of another yeast plasmid bearing a different origin, ARS305, also exhibited random termination. We propose that the random termination events observed in S.cerevisiae could be due to an asynchronous departure of both forks from the bi-directional origin in addition to differences in the rate of fork progression. These observations could be extended to all bi-directional origins.
Auborn,
Replicative intermediates of human papillomavirus type 11 in laryngeal papillomas: site of replication initiation and direction of replication.
1994, Pubmed
Auborn,
Replicative intermediates of human papillomavirus type 11 in laryngeal papillomas: site of replication initiation and direction of replication.
1994,
Pubmed Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992,
Pubmed Bielinsky,
Chromosomal ARS1 has a single leading strand start site.
1999,
Pubmed Bielinsky,
Discrete start sites for DNA synthesis in the yeast ARS1 origin.
1998,
Pubmed Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Brewer,
Initiation preference at a yeast origin of replication.
1994,
Pubmed Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed Brun,
Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels.
1995,
Pubmed Buckler-White,
Asynchronous bidirectional replication of polyoma virus DNA.
1982,
Pubmed Caddle,
Specific initiation at an origin of replication from Schizosaccharomyces pombe.
1994,
Pubmed Celniker,
Yeast DNA replication in vitro: initiation and elongation events mimic in vivo processes.
1982,
Pubmed Diffley,
Two steps in the assembly of complexes at yeast replication origins in vivo.
1994,
Pubmed
,
Xenbase Dijkwel,
Mapping replication origins by neutral/neutral two-dimensional gel electrophoresis.
1997,
Pubmed Duncker,
Cyclin B-cdk1 kinase stimulates ORC- and Cdc6-independent steps of semiconservative plasmid replication in yeast nuclear extracts.
1999,
Pubmed
,
Xenbase Friedman,
Analysis of replication intermediates by two-dimensional agarose gel electrophoresis.
1995,
Pubmed Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Hay,
Initiation of SV40 DNA replication in vivo: location and structure of 5' ends of DNA synthesized in the ori region.
1982,
Pubmed Hendrickson,
The origin of bidirectional DNA replication in polyoma virus.
1987,
Pubmed Huberman,
The in vivo replication origin of the yeast 2 microns plasmid.
1987,
Pubmed Hyrien,
Plasmid replication in Xenopus eggs and egg extracts: a 2D gel electrophoretic analysis.
1992,
Pubmed
,
Xenbase Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase Kaguni,
Replication initiated at the origin (oriC) of the E. coli chromosome reconstituted with purified enzymes.
1984,
Pubmed Krysan,
Replication initiates at multiple locations on an autonomously replicating plasmid in human cells.
1991,
Pubmed LaDuca,
Site-specific pausing of deoxyribonucleic acid synthesis catalyzed by four forms of Escherichia coli DNA polymerase III.
1983,
Pubmed Lee,
Architecture of the yeast origin recognition complex bound to origins of DNA replication.
1997,
Pubmed Levac,
Inactivation of topoisomerase I or II may lead to recombination or to aberrant replication termination on both SV40 and yeast 2 micron DNA.
1996,
Pubmed Li,
Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication.
1985,
Pubmed Little,
Initiation and termination of DNA replication in human rRNA genes.
1993,
Pubmed Mahbubani,
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Martín-Parras,
Topological complexity of different populations of pBR322 as visualized by two-dimensional agarose gel electrophoresis.
1998,
Pubmed
,
Xenbase Martín-Parras,
Initiation of DNA replication in ColE1 plasmids containing multiple potential origins of replication.
1992,
Pubmed Martín-Parras,
Unidirectional replication as visualized by two-dimensional agarose gel electrophoresis.
1991,
Pubmed Miyata,
Asymmetrical progression of replication forks just after initiation on Mycoplasma capricolum chromosome revealed by two-dimensional gel electrophoresis.
1997,
Pubmed Moriya,
Mapping of the replication origin of the Bacillus subtilis chromosome by the two-dimensional gel method.
1996,
Pubmed Newlon,
Putting it all together: building a prereplicative complex.
1997,
Pubmed
,
Xenbase Newlon,
Analysis of a circular derivative of Saccharomyces cerevisiae chromosome III: a physical map and identification and location of ARS elements.
1991,
Pubmed Santamaría,
DnaB helicase is unable to dissociate RNA-DNA hybrids. Its implication in the polar pausing of replication forks at ColE1 origins.
1998,
Pubmed Santocanale,
ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae.
1996,
Pubmed Schvartzman,
The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis.
1993,
Pubmed Schvartzman,
Evidence that replication initiates at only some of the potential origins in each oligomeric form of bovine papillomavirus type 1 DNA.
1990,
Pubmed Shishido,
Increased production of a knotted form of plasmid pBR322 DNA in Escherichia coli DNA topoisomerase mutants.
1987,
Pubmed Shishido,
The presence of the region on pBR322 that encodes resistance to tetracycline is responsible for high levels of plasmid DNA knotting in Escherichia coli DNA topoisomerase I deletion mutant.
1989,
Pubmed Tack,
Two major replicating simian virus 40 chromosome classes. Synchronous replication fork movement is associated with bound large T antigen during elongation.
1987,
Pubmed Tapper,
Preferred DNA sites are involved in the arrest and initiation of DNA synthesis during replication of SV40 DNA.
1980,
Pubmed Tapper,
Discontinuous DNA replication: accumulation of Simian virus 40 DNA at specific stages in its replication.
1978,
Pubmed Tomizawa,
Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.
1974,
Pubmed Vaughn,
Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain.
1990,
Pubmed Viguera,
The ColE1 unidirectional origin acts as a polar replication fork pausing site.
1996,
Pubmed Viguera,
A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis.
1998,
Pubmed Yoshimoto,
Early replicative intermediates of Escherichia coli chromosome isolated from a membrane complex.
1986,
Pubmed Yoshimoto,
Asymmetric replication of an oriC plasmid in Escherichia coli.
1986,
Pubmed Zhu,
Localization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae.
1992,
Pubmed
