Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
During oocyte maturation in Xenopus, previously quiescent maternal mRNAs are translationally activated at specific times. We hypothesized that the translational recruitment of individual messages is triggered by particular cellular events and investigated the potential for known effectors of the meiotic cell cycle to activate the translation of the FGF receptor-1 (XFGFR) maternal mRNA. We found that both c-mos and cdc2 activate the translation of XFGFR. However, although oocytes matured by injection of recombinant cdc2/cyclin B translate normal levels of XFGFR protein, c-mos depletion reduces the level of XFGFR protein induced by cdc2/cyclin B injection. In oocytes blocked for cdc2 activity, injection of mos RNA induced low levels of XFGFR protein, independent of MAPK activity. Through the use of injected reporter RNAs, we show that the XFGFR 3' untranslated region inhibitory element is completely derepressed by cdc2 alone. In addition, we identified a new inhibitory element through which both mos and cdc2 activate translation. We found that cdc2 derepresses translation in the absence of polyadenylation, whereas mos requires poly(A) extension to activate XFGFR translation. Our results demonstrate that mos and cdc2, in addition to functioning as key regulators of the meiotic cell cycle, cooperate in the translational activation of a specific maternal mRNA during oocyte maturation.
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991, Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase Amaya,
FGF signalling in the early specification of mesoderm in Xenopus.
1993,
Pubmed
,
Xenbase Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase Bu,
Novel phosphorylation sites of eukaryotic initiation factor-4F and evidence that phosphorylation stabilizes interactions of the p25 and p220 subunits.
1993,
Pubmed Colgan,
Cell-cycle related regulation of poly(A) polymerase by phosphorylation.
1996,
Pubmed
,
Xenbase Colgan,
Inhibition of poly(A) polymerase requires p34cdc2/cyclin B phosphorylation of multiple consensus and non-consensus sites.
1998,
Pubmed
,
Xenbase Culp,
Translational activation and cytoplasmic polyadenylation of FGF receptor-1 are independently regulated during Xenopus oocyte maturation.
1998,
Pubmed
,
Xenbase Daar,
A characterization of cytostatic factor activity from Xenopus eggs and c-mos-transformed cells.
1991,
Pubmed
,
Xenbase de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Fajardo,
Separate elements in the 3' untranslated region of the mouse protamine 1 mRNA regulate translational repression and activation during murine spermatogenesis.
1997,
Pubmed Ferrell,
The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes.
1998,
Pubmed
,
Xenbase Flynn,
Insulin-stimulated phosphorylation of initiation factor 4E is mediated by the MAP kinase pathway.
1996,
Pubmed Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Fraser,
The association of initiation factor 4F with poly(A)-binding protein is enhanced in serum-stimulated Xenopus kidney cells.
1999,
Pubmed
,
Xenbase Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Goodwin,
Translational regulation of tra-2 by its 3' untranslated region controls sexual identity in C. elegans.
1993,
Pubmed Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase Gunkel,
Localization-dependent translation requires a functional interaction between the 5' and 3' ends of oskar mRNA.
1998,
Pubmed Haccard,
Induction of Xenopus oocyte meiotic maturation by MAP kinase.
1995,
Pubmed
,
Xenbase Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Huang,
Dependence of Mos-induced Cdc2 activation on MAP kinase function in a cell-free system.
1996,
Pubmed
,
Xenbase Huang,
Biochemical and biological analysis of Mek1 phosphorylation site mutants.
1995,
Pubmed
,
Xenbase Huchon,
Control of metaphase I formation in Xenopus oocyte: effects of an indestructible cyclin B and of protein synthesis.
1993,
Pubmed
,
Xenbase Jan,
Conservation of the C.elegans tra-2 3'UTR translational control.
1997,
Pubmed Jan,
The STAR protein, GLD-1, is a translational regulator of sexual identity in Caenorhabditis elegans.
1999,
Pubmed Kanki,
Progression from meiosis I to meiosis II in Xenopus oocytes requires de novo translation of the mosxe protooncogene.
1991,
Pubmed
,
Xenbase Kaufman,
Control of gene expression at the level of translation initiation.
1994,
Pubmed Keiper,
Translational recruitment of Xenopus maternal mRNAs in response to poly(A) elongation requires initiation factor eIF4G-1.
1999,
Pubmed
,
Xenbase Kim-Ha,
Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential.
1995,
Pubmed Lawrence,
PHAS/4E-BPs as regulators of mRNA translation and cell proliferation.
1997,
Pubmed Matten,
Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.
1996,
Pubmed
,
Xenbase McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase Minshall,
Dual roles of p82, the clam CPEB homolog, in cytoplasmic polyadenylation and translational masking.
1999,
Pubmed
,
Xenbase Morley,
Increased phosphorylation of eukaryotic initiation factor 4 alpha during early activation of T lymphocytes correlates with increased initiation factor 4F complex formation.
1993,
Pubmed Morley,
Hormone-induced meiotic maturation in Xenopus oocytes occurs independently of p70s6k activation and is associated with enhanced initiation factor (eIF)-4F phosphorylation and complex formation.
1995,
Pubmed
,
Xenbase Morris,
Growth control of translation in mammalian cells.
1995,
Pubmed Murata,
Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos.
1995,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase Nebreda,
The protein kinase mos activates MAP kinase kinase in vitro and stimulates the MAP kinase pathway in mammalian somatic cells in vivo.
1993,
Pubmed
,
Xenbase Nebreda,
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
1995,
Pubmed
,
Xenbase Nishizawa,
The 'second-codon rule' and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Ostareck-Lederer,
Translation of 15-lipoxygenase mRNA is inhibited by a protein that binds to a repeated sequence in the 3' untranslated region.
1994,
Pubmed Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Robbie,
Temporal regulation of the Xenopus FGF receptor in development: a translation inhibitory element in the 3' untranslated region.
1995,
Pubmed
,
Xenbase Roy,
Mos proto-oncogene function during oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Rupp,
Xenopus embryos regulate the nuclear localization of XMyoD.
1994,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
,
Xenbase Standart,
Maternal mRNA from clam oocytes can be specifically unmasked in vitro by antisense RNA complementary to the 3'-untranslated region.
1990,
Pubmed Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Sun,
MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.
1993,
Pubmed Tarun,
Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G.
1996,
Pubmed Walker,
The clam 3' UTR masking element-binding protein p82 is a member of the CPEB family.
1999,
Pubmed
,
Xenbase Walker,
Unmasking mRNA in clam oocytes: role of phosphorylation of a 3' UTR masking element-binding protein at fertilization.
1996,
Pubmed Wasserman,
Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytes.
1982,
Pubmed
,
Xenbase Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase Zhang,
A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line.
1997,
Pubmed
