Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Functional modifications of neuronal P/Q-type voltage-dependent Ca2+ channels expressed in Xenopus oocytes by oxidation were examined electrophysiologically. Oxidation by external H2O2 enhanced the whole-oocyte currents through the Ca2+ channels composed of the alpha1A, alpha2/delta, and beta3 subunits at negative voltages (<0 mV) without markedly affecting the currents at more positive voltages. Single-channel analysis showed that oxidation accelerates the overall channel opening process. The effect of H2O2 to enhance the Ca2+ channel activity did not require heterologous expression of the alpha2/delta subunit, and it was not mimicked by a cysteine-specific oxidizing agent. The results suggest that oxidative stress may regulate the activity of neuronal Ca2+ channels and that regulation by oxidation may be important in some clinical situations, such as in reperfusion injury after ischemic episodes.
Aldrich,
A reinterpretation of mammalian sodium channel gating based on single channel recording.
, Pubmed
Aldrich,
A reinterpretation of mammalian sodium channel gating based on single channel recording.
,
Pubmed Babbs,
Reperfusion injury of postischemic tissues.
1988,
Pubmed Bean,
Neurotransmitter inhibition of neuronal calcium currents by changes in channel voltage dependence.
1989,
Pubmed Beckman,
Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide.
1990,
Pubmed Birnbaumer,
The naming of voltage-gated calcium channels.
1994,
Pubmed Chan,
Role of oxidants in ischemic brain damage.
1996,
Pubmed Chiamvimonvat,
Functional consequences of sulfhydryl modification in the pore-forming subunits of cardiovascular Ca2+ and Na+ channels.
1995,
Pubmed
,
Xenbase Ciorba,
Modulation of potassium channel function by methionine oxidation and reduction.
1997,
Pubmed
,
Xenbase Dawson,
Nitric oxide neurotoxicity.
1996,
Pubmed De Waard,
Direct binding of G-protein betagamma complex to voltage-dependent calcium channels.
1997,
Pubmed
,
Xenbase De Waard,
Structural and functional diversity of voltage-activated calcium channels.
1996,
Pubmed De Waard,
Subunit regulation of the neuronal alpha 1A Ca2+ channel expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase Dolphin,
Facilitation of Ca2+ current in excitable cells.
1996,
Pubmed Duprat,
Susceptibility of cloned K+ channels to reactive oxygen species.
1995,
Pubmed
,
Xenbase Ellis,
Sequence and expression of mRNAs encoding the alpha 1 and alpha 2 subunits of a DHP-sensitive calcium channel.
1988,
Pubmed Gorman,
Oxidative stress and apoptosis in neurodegeneration.
1996,
Pubmed Gress,
Stroke. Revolution in therapy.
1994,
Pubmed Halliwell,
Reactive oxygen species and the central nervous system.
1992,
Pubmed Hensley,
Reactive oxygen species as causal agents in the neurotoxicity of the Alzheimer's disease-associated amyloid beta peptide.
1996,
Pubmed Hescheler,
G-proteins involved in the calcium channel signalling system.
1993,
Pubmed Hofmann,
Molecular basis for Ca2+ channel diversity.
1994,
Pubmed Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase Hoshi,
Regulation of voltage dependence of the KAT1 channel by intracellular factors.
1995,
Pubmed
,
Xenbase Hullin,
Calcium channel beta subunit heterogeneity: functional expression of cloned cDNA from heart, aorta and brain.
1992,
Pubmed
,
Xenbase Islam,
Sulfhydryl oxidation induces rapid and reversible closure of the ATP-regulated K+ channel in the pancreatic beta-cell.
1993,
Pubmed Jenner,
Oxidative stress and the pathogenesis of Parkinson's disease.
1996,
Pubmed Llinás,
Distribution and functional significance of the P-type, voltage-dependent Ca2+ channels in the mammalian central nervous system.
1992,
Pubmed
,
Xenbase López-Barneo,
Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels.
1993,
Pubmed
,
Xenbase Mori,
Primary structure and functional expression from complementary DNA of a brain calcium channel.
1991,
Pubmed
,
Xenbase Multhaup,
Reactive oxygen species and Alzheimer's disease.
1997,
Pubmed Olanow,
Metals and free radicals in neurodegeneration.
1994,
Pubmed Oyama,
Oxidative stress-induced increase in intracellular Ca2+ and Ca(2+)-induced increase in oxidative stress: an experimental model using dissociated rat brain neurons.
1996,
Pubmed Park,
Different modulation of Ca-activated K channels by the intracellular redox potential in pulmonary and ear arterial smooth muscle cells of the rabbit.
1995,
Pubmed Porter,
Mechanisms of free radical oxidation of unsaturated lipids.
1995,
Pubmed Pragnell,
Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit.
1994,
Pubmed Ruppersberg,
Regulation of fast inactivation of cloned mammalian IK(A) channels by cysteine oxidation.
1991,
Pubmed
,
Xenbase Schlief,
Modification of C-type inactivating Shaker potassium channels by chloramine-T.
1996,
Pubmed
,
Xenbase Sculptoreanu,
Voltage-dependent potentiation of L-type Ca2+ channels due to phosphorylation by cAMP-dependent protein kinase.
1993,
Pubmed Stephens,
Cysteine-modifying reagents alter the gating of the rat cloned potassium channel Kv1.4.
1996,
Pubmed Suzuki,
Oxidants as stimulators of signal transduction.
1997,
Pubmed Taglialatela,
Regulation of the human ether-a-gogo related gene (HERG) K+ channels by reactive oxygen species.
1997,
Pubmed
,
Xenbase Tokube,
Openings of cardiac KATP channel by oxygen free radicals produced by xanthine oxidase reaction.
1996,
Pubmed Uchitel,
P-type voltage-dependent calcium channel mediates presynaptic calcium influx and transmitter release in mammalian synapses.
1992,
Pubmed Werz,
Phosphorylation enhances inactivation of N-type calcium channel current in bullfrog sympathetic neurons.
1993,
Pubmed Xia,
Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury.
1996,
Pubmed Yang,
Enhancement of N- and L-type calcium channel currents by protein kinase C in frog sympathetic neurons.
1993,
Pubmed Zagotta,
Gating of single Shaker potassium channels in Drosophila muscle and in Xenopus oocytes injected with Shaker mRNA.
1989,
Pubmed
,
Xenbase Zamponi,
Crosstalk between G proteins and protein kinase C mediated by the calcium channel alpha1 subunit.
1997,
Pubmed
,
Xenbase Zhang,
Multiple structural elements in voltage-dependent Ca2+ channels support their inhibition by G proteins.
1996,
Pubmed
,
Xenbase
