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Mol Cell Biol
1998 Feb 01;182:906-18. doi: 10.1128/MCB.18.2.906.
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Intramolecular repression of mouse heat shock factor 1.
Farkas T, Kutskova YA, Zimarino V.
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The pathway leading to transcriptional activation of heat shock genes involves a step of heat shock factor 1 (HSF1) trimerization required for high-affinity binding of this activator protein to heat shock elements (HSEs) in the promoters. Previous studies have shown that in vivo the trimerization is negatively regulated at physiological temperatures by a mechanism that requires multiple hydrophobic heptad repeats (HRs) which may form a coiled coil in the monomer. To investigate the minimal requirements for negative regulation, in this work we have examined mouse HSF1 translated in rabbit reticulocyte lysate or extracted from Escherichia coli after limited expression. We show that under these conditions HSF1 behaves as a monomer which can be induced by increases in temperature to form active HSE-binding trimers and that mutations of either HR region cause activation in both systems. Furthermore, temperature elevations and acidic buffers activate purified HSF1, and mild proteolysis excises fragments which form HSE-binding oligomers. These results suggest that oligomerization can be repressed in the monomer, as previously proposed, and that repression can be relieved in the apparent absence of regulatory proteins. An intramolecular mechanism may be central for the regulation of this transcription factor in mammalian cells, although not necessarily sufficient.
Abdella,
A new cleavable reagent for cross-linking and reversible immobilization of proteins.
1979, Pubmed
Abdella,
A new cleavable reagent for cross-linking and reversible immobilization of proteins.
1979,
Pubmed Abravaya,
The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression.
1992,
Pubmed Ananthan,
Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes.
1986,
Pubmed
,
Xenbase Baler,
Activation of human heat shock genes is accompanied by oligomerization, modification, and rapid translocation of heat shock transcription factor HSF1.
1993,
Pubmed
,
Xenbase Baler,
Evidence for a role of Hsp70 in the regulation of the heat shock response in mammalian cells.
1996,
Pubmed Clos,
Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation.
1990,
Pubmed
,
Xenbase Clos,
Induction temperature of human heat shock factor is reprogrammed in a Drosophila cell environment.
1993,
Pubmed de la Brousse,
Glimpses of allostery in the control of eukaryotic gene expression.
1993,
Pubmed Fernandes,
Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions.
1994,
Pubmed Fiorenza,
Complex expression of murine heat shock transcription factors.
1995,
Pubmed Gething,
Protein folding in the cell.
1992,
Pubmed Goodson,
Heat-inducible DNA binding of purified heat shock transcription factor 1.
1995,
Pubmed Green,
A heat shock-responsive domain of human HSF1 that regulates transcription activation domain function.
1995,
Pubmed Harrison,
Crystal structure of the DNA binding domain of the heat shock transcription factor.
1994,
Pubmed Hübel,
Arabidopsis heat shock factor is constitutively active in Drosophila and human cells.
1995,
Pubmed Jurivich,
Effect of sodium salicylate on the human heat shock response.
1992,
Pubmed Kim,
Interaction of the DNA-binding domain of Drosophila heat shock factor with its cognate DNA site: a thermodynamic analysis using analytical ultracentrifugation.
1994,
Pubmed Knauf,
Repression of human heat shock factor 1 activity at control temperature by phosphorylation.
1996,
Pubmed Kroeger,
Mouse heat shock transcription factors 1 and 2 prefer a trimeric binding site but interact differently with the HSP70 heat shock element.
1993,
Pubmed Larson,
In vitro activation of purified human heat shock factor by heat.
1995,
Pubmed Larson,
Activation in vitro of sequence-specific DNA binding by a human regulatory factor.
1988,
Pubmed Lindquist,
The heat-shock response.
1986,
Pubmed Lis,
Protein traffic on the heat shock promoter: parking, stalling, and trucking along.
1993,
Pubmed Mosser,
The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by hsp70.
1993,
Pubmed Mosser,
In vitro activation of heat shock transcription factor DNA-binding by calcium and biochemical conditions that affect protein conformation.
1990,
Pubmed Nakai,
HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator.
1997,
Pubmed Nakai,
Characterization of a novel chicken heat shock transcription factor, heat shock factor 3, suggests a new regulatory pathway.
1993,
Pubmed Newton,
The regulatory domain of human heat shock factor 1 is sufficient to sense heat stress.
1996,
Pubmed Orosz,
Regulation of Drosophila heat shock factor trimerization: global sequence requirements and independence of nuclear localization.
1996,
Pubmed Peteranderl,
Trimerization of the heat shock transcription factor by a triple-stranded alpha-helical coiled-coil.
1992,
Pubmed Rabindran,
Regulation of heat shock factor trimer formation: role of a conserved leucine zipper.
1993,
Pubmed Rabindran,
Molecular cloning and expression of a human heat shock factor, HSF1.
1991,
Pubmed Rabindran,
Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo.
1994,
Pubmed Sarge,
Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability.
1991,
Pubmed Sarge,
Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress.
1993,
Pubmed Schultheiss,
Solution structure of the DNA-binding domain of the tomato heat-stress transcription factor HSF24.
1996,
Pubmed Shi,
The carboxyl-terminal transactivation domain of heat shock factor 1 is negatively regulated and stress responsive.
1995,
Pubmed Sorger,
Trimerization of a yeast transcriptional activator via a coiled-coil motif.
1989,
Pubmed Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed Treuter,
Promoter specificity and deletion analysis of three heat stress transcription factors of tomato.
1993,
Pubmed Vuister,
NMR evidence for similarities between the DNA-binding regions of Drosophila melanogaster heat shock factor and the helix-turn-helix and HNF-3/forkhead families of transcription factors.
1994,
Pubmed Westwood,
Activation of Drosophila heat shock factor: conformational change associated with a monomer-to-trimer transition.
1993,
Pubmed Westwood,
Stress-induced oligomerization and chromosomal relocalization of heat-shock factor.
1991,
Pubmed Wu,
Heat shock transcription factors: structure and regulation.
1995,
Pubmed Zimarino,
Antibody-mediated activation of Drosophila heat shock factor in vitro.
1990,
Pubmed Zimarino,
Induction of sequence-specific binding of Drosophila heat shock activator protein without protein synthesis.
,
Pubmed Zimarino,
Complex modes of heat shock factor activation.
1990,
Pubmed Zuo,
Multiple layers of regulation of human heat shock transcription factor 1.
1995,
Pubmed
,
Xenbase Zuo,
Activation of the DNA-binding ability of human heat shock transcription factor 1 may involve the transition from an intramolecular to an intermolecular triple-stranded coiled-coil structure.
1994,
Pubmed
,
Xenbase
