Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
1996 Feb 01;4314:645-51. doi: 10.1007/bf02191915.
Show Gene links
Show Anatomy links
Expression of the renal Na+/dicarboxylate cotransporter, NaDC-1, in COS-7 cells.
Pajor AM, Valmonte HG.
???displayArticle.abstract???
The cloned rabbit renal Na+/dicarboxylate cotransporter, NaDC-1, was transiently expressed in the mammalian monkey kidney cell line, COS-7. Cells transfected with the plasmid pSV-201, containing the cDNA for the Na+/dicarboxylate cotransporter, expressed sodium-dependent succinate and citrate transport after 48 h. Cells transfected with control plasmid, pSV-SPORT, did not express sodium-dependent transport of succinate or citrate. The transport of succinate in cells expressing NaDC-1 was inhibited by di-and tricarboxylic acids, but not by monocarboxylic acids. Sodium-dependent transport of succinate was insensitive to changes in pH, while sodium-dependent citrate transport was stimulated by acidic pH. Succinate transport by NaDC-1 was saturable with an apparent Michaelis constant, Km, around 0.5 mM. The kinetics of sodium activation of succinate transport by NaDC-1 were sigmoidal, with an apparent Hill coefficient of 2.9, indicating that three sodium ions are involved in the transport of each succinate. Succinate transport by NaDC-1 was inhibited by lithium. The functional characteristics of NaDC-1 expressed in COS-7 cells correspond to those of the Na+/dicarboxylate cotransporter of the apical membrane of the renal proximal tubule.
Birnir,
Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.
1990, Pubmed,
Xenbase
Birnir,
Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.
1990,
Pubmed
,
Xenbase Brennan,
Effect of pH on citrate reabsorption in the proximal convoluted tubule.
1988,
Pubmed Burckhardt,
Sodium-dependent dicarboxylate transport in rat renal basolateral membrane vesicles.
1984,
Pubmed Busch,
Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+SO4(2-) transport protein NaSi-1.
1994,
Pubmed
,
Xenbase Domin,
Purine nucleobase transport in human erythrocytes. Reinvestigation with a novel "inhibitor-stop" assay.
1988,
Pubmed Hamm,
Renal handling of citrate.
1990,
Pubmed Hirayama,
Coupling between sodium and succinate transport across renal brush border membrane vesicles.
1986,
Pubmed Jenkins,
Transport of citrate across renal brush border membrane: effects of dietary acid and alkali loading.
1985,
Pubmed Levi,
Chronic K depletion stimulates rat renal brush-border membrane Na-citrate cotransporter.
1991,
Pubmed Markovich,
Expression cloning of rat renal Na+/SO4(2-) cotransport.
1993,
Pubmed
,
Xenbase Pajor,
Sequence and functional characterization of a renal sodium/dicarboxylate cotransporter.
1995,
Pubmed
,
Xenbase Pak,
Etiology and treatment of urolithiasis.
1991,
Pubmed Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase Smith,
Baculovirus-mediated expression of the Na+/glucose cotransporter in Sf9 cells.
1992,
Pubmed Windus,
Effects of fasting on citrate transport by the brush-border membrane of rat kidney.
1986,
Pubmed Wright,
Kinetics of sodium succinate cotransport across renal brush-border membranes.
1983,
Pubmed Wright,
Transport of carboxylic acids by renal membrane vesicles.
1985,
Pubmed Wright,
Effect of pH on the transport of Krebs cycle intermediates in renal brush border membranes.
1982,
Pubmed Wright,
Succinate and citrate transport in renal basolateral and brush-border membranes.
1987,
Pubmed
