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Chromosoma
1995 May 01;1039:597-605. doi: 10.1007/bf00357686.
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Autonomous replication in Drosophila melanogaster tissue culture cells.
Smith JG, Calos MP.
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This study addresses the ability of DNA fragments from various sources to mediate autonomous DNA replication in cultured Drosophila melanogaster cells. We created a series of plasmids containing genomic DNA fragments from the Ultrabithorax gene of Drosophila and test ed them for autonomous replication after transfection into Schneider line 2 cells. We found that all plasmids containing Drosophila DNA were able to replicate autonomously, as were random human and Escherichia coli genomic DNA fragments. Most of the plasmids were detectable 18 days after transfection in the absence of selection, suggesting that transfected DNA is maintained in Drosophila cells without rapid loss or degradation. The finding that all plasmids containing Drosophila, human or bacterial DNA replicate autonomously in Drosophila cells suggests that the signals that direct autonomous replication in Drosophila contain a low degree of sequence specificity. A two-dimensional gel analysis of initiation on one of the plasmids was consistent with many dispersed initiation sites. Low sequence specificity and dispersed initiation sites also characterize autonomous replication in human cells and Xenopus eggs and may be general properties of autonomous replication in animal cells.
Amati,
Drosophila scaffold-attached regions bind nuclear scaffolds and can function as ARS elements in both budding and fission yeasts.
1990, Pubmed
Amati,
Drosophila scaffold-attached regions bind nuclear scaffolds and can function as ARS elements in both budding and fission yeasts.
1990,
Pubmed Bender,
Molecular Genetics of the Bithorax Complex in Drosophila melanogaster.
1983,
Pubmed Blumenthal,
The units of DNA replication in Drosophila melanogaster chromosomes.
1974,
Pubmed Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed Burhans,
Identification of an origin of bidirectional DNA replication in mammalian chromosomes.
1990,
Pubmed Caddle,
Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication.
1992,
Pubmed Crevel,
DNA replication in cell-free extracts from Drosophila melanogaster.
1991,
Pubmed
,
Xenbase Delidakis,
Amplification enhancers and replication origins in the autosomal chorion gene cluster of Drosophila.
1989,
Pubmed Dijkwel,
Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine.
1992,
Pubmed Fuchs,
Nuclear structure: determination of the fate of the nuclear envelope in Drosophila during mitosis using monoclonal antibodies.
1983,
Pubmed Gahn,
The Epstein-Barr virus origin of plasmid replication, oriP, contains both the initiation and termination sites of DNA replication.
1989,
Pubmed Haase,
Replication control of autonomously replicating human sequences.
1991,
Pubmed Haase,
Transcription inhibits the replication of autonomously replicating plasmids in human cells.
1994,
Pubmed Handeli,
Mapping replication units in animal cells.
1989,
Pubmed Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Heck,
Multiple replication origins are used during Drosophila chorion gene amplification.
1990,
Pubmed Heinzel,
Autonomous DNA replication in human cells is affected by the size and the source of the DNA.
1991,
Pubmed Hirt,
Selective extraction of polyoma DNA from infected mouse cell cultures.
1967,
Pubmed Hsiao,
High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.
1979,
Pubmed Huberman,
The in vivo replication origin of the yeast 2 microns plasmid.
1987,
Pubmed Hyrien,
Plasmid replication in Xenopus eggs and egg extracts: a 2D gel electrophoretic analysis.
1992,
Pubmed
,
Xenbase Jongens,
Functional redundancy in the tissue-specific enhancer of the Drosophila Sgs-4 gene.
1988,
Pubmed Kelly,
SV40 DNA replication.
1988,
Pubmed Kornfeld,
Structure and expression of a family of Ultrabithorax mRNAs generated by alternative splicing and polyadenylation in Drosophila.
1989,
Pubmed Krasnow,
Transcriptional activation and repression by Ultrabithorax proteins in cultured Drosophila cells.
1989,
Pubmed Krysan,
Autonomous replication in human cells of multimers of specific human and bacterial DNA sequences.
1993,
Pubmed Krysan,
Isolation of human sequences that replicate autonomously in human cells.
1989,
Pubmed Krysan,
Replication initiates at multiple locations on an autonomously replicating plasmid in human cells.
1991,
Pubmed Liang,
Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method.
1994,
Pubmed Liang,
Replication initiates at a confined region during DNA amplification in Sciara DNA puff II/9A.
1993,
Pubmed Mahbubani,
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Martin,
A transient expression assay for tissue-specific gene expression of alcohol dehydrogenase in Drosophila.
1986,
Pubmed Méchali,
Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast.
1984,
Pubmed
,
Xenbase Newlon,
Yeast chromosome replication and segregation.
1988,
Pubmed Orr-Weaver,
Drosophila chorion genes: cracking the eggshell's secrets.
1991,
Pubmed Roth,
Replication analysis of plasmid DNAs injected into Drosophila embryos.
1991,
Pubmed Schneider,
Cell lines derived from late embryonic stages of Drosophila melanogaster.
1972,
Pubmed Shinomiya,
Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone.
1994,
Pubmed Shinomiya,
Analysis of chromosomal replicons in early embryos of Drosophila melanogaster by two-dimensional gel electrophoresis.
1991,
Pubmed Shinomiya,
DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.
1993,
Pubmed Stinchcomb,
Isolation and characterisation of a yeast chromosomal replicator.
1979,
Pubmed Tran,
The replication behavior of Saccharomyces cerevisiae DNA in human cells.
1993,
Pubmed Urieli-Shoval,
The absence of detectable methylated bases in Drosophila melanogaster DNA.
1982,
Pubmed Vaughn,
Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain.
1990,
Pubmed Waye,
Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome.
1986,
Pubmed Yang,
Replication of bovine papillomavirus type 1 DNA initiates within an E2-responsive enhancer element.
1990,
Pubmed Yasuda,
Cloning and mapping of the replication origin of Escherichia coli.
1977,
Pubmed Yates,
A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells.
1984,
Pubmed
