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J Mol Neurosci
1995 Jan 01;64:249-55. doi: 10.1007/bf02736784.
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Three related brain nuclear receptors, NGFI-B, Nurr1, and NOR-1, as transcriptional activators.
Paulsen RF
,
Granas K
,
Johnsen H
,
Rolseth V
,
Sterri S
.
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Three related orphan nuclear receptors that are expressed in the brain, NGFI-B, Nurr1, and NOR-1, were studied to compare their function as transcriptional activators. NGFI-B was able to activate (in the absence of added hormone) in CV1 cells both an NGFI-B-responsive luciferase reporter gene (containing eight copies of a response element for NGFI-B upstream of a basal prolactin promoter driving the luciferase gene, NBRE(8)-LUC), a similar thyroid hormone-receptor-responsive reporter gene (TRE(3)-LUC), and a reporter gene with an authentic promoter from a Xenopus vitellogenin gene containing two binding sites for the estrogen receptor (vit-LUC). NGFI-B activated NBRE(8)-LUC and TRE(3)-LUC (but not the vitLUC) with an amino-terminal activation domain. Nurr1 was less promiscuous as a transcriptional activator, activating.the NBRE(8)-LUC better than NGFI-B, but less than NGFI-B at the other reporter genes. NOR-1 activated only the NBRE(8)-LUC reporter gene. These results indicate that closely related nuclear receptors may differentiate between response elements or promoters and that different activation mechanisms exist depending on the promoter. This may contribute to regulation of specificity of target gene expression in the brain.
Beato,
Gene regulation by steroid hormones.
1989, Pubmed
Beato,
Gene regulation by steroid hormones.
1989,
Pubmed
Ciani,
Activation of a reporter gene responsive to NGFI-B in cultured neurons and astrocytes.
1995,
Pubmed
Evans,
The steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Freedman,
On the mechanism of DNA binding by nuclear hormone receptors: a structural and functional perspective.
1993,
Pubmed
Hazel,
Nur77 is differentially modified in PC12 cells upon membrane depolarization and growth factor treatment.
1991,
Pubmed
Hazel,
A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Kumar,
Functional domains of the human estrogen receptor.
1987,
Pubmed
Law,
Identification of a new brain-specific transcription factor, NURR1.
1992,
Pubmed
Lee,
Unimpaired thymic and peripheral T cell death in mice lacking the nuclear receptor NGFI-B (Nur77).
1995,
Pubmed
Liu,
Apoptotic signals delivered through the T-cell receptor of a T-cell hybrid require the immediate-early gene nur77.
1994,
Pubmed
Milbrandt,
Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene.
1988,
Pubmed
Mitchell,
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
1989,
Pubmed
Ohkura,
Molecular cloning of a novel thyroid/steroid receptor superfamily gene from cultured rat neuronal cells.
1994,
Pubmed
Paulsen,
Domains regulating transcriptional activity of the inducible orphan receptor NGFI-B.
1992,
Pubmed
Sadowski,
A vector for expressing GAL4(1-147) fusions in mammalian cells.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Seed,
A simple phase-extraction assay for chloramphenicol acyltransferase activity.
1988,
Pubmed
Watson,
Expression of the nerve growth factor-regulated NGFI-A and NGFI-B genes in the developing rat.
1990,
Pubmed
Watson,
The NGFI-B gene, a transcriptionally inducible member of the steroid receptor gene superfamily: genomic structure and expression in rat brain after seizure induction.
1989,
Pubmed
Wilson,
Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors.
1992,
Pubmed
Wilson,
Identification of the DNA binding site for NGFI-B by genetic selection in yeast.
1991,
Pubmed
Winoto,
Molecular characterization of the Nur77 orphan steroid receptor in apoptosis.
1994,
Pubmed
Woronicz,
Requirement for the orphan steroid receptor Nur77 in apoptosis of T-cell hybridomas.
1994,
Pubmed
de Wet,
Firefly luciferase gene: structure and expression in mammalian cells.
1987,
Pubmed