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To investigate the physiological role of a kidney-specific chloride channel (ClC-K1), we sought to determine its exact localization by immunohistochemistry and its functional regulation using Xenopus oocyte expression system. The antiserum specifically recognized a 70-kD protein in SDS-PAGE of membrane protein from rat inner medulla and an in vitro translated ClC-K1 protein. Immunohistochemistry revealed that ClC-K1 was exclusively localized to the thin limb of Henle's loop in rat inner medulla. In comparison with the immunostaining with anti-aquaporin-CHIP antibody that only stains the descending thin limb of Henle's loop (tDL), ClC-K1 was found to be localized only in the ascending limb (tAL) which has the highest chloride permeability among nephron segments. Immunoelectron microscopy confirmed that the staining of ClC-K1 in tAL was observed in the region of both apical and basolateral plasma membranes. Expressed chloride current in Xenopus oocytes by ClC-K1 cRNA was regulated by extracellular pH and extracellular calcium. Furosemide inhibited the expressed current (Ki = 100 microM), whereas N-ethyl-maleimide stimulated the current. These functional characteristics were consistent with the in vitro perfusion studies of chloride transport in tAL. The localization and the functional characteristics described here indicate that ClC-K1 is responsible for the transepithelial chloride transport in tAL.
Biemesderfer,
NHE3: a Na+/H+ exchanger isoform of renal brush border.
1993, Pubmed
Biemesderfer,
NHE3: a Na+/H+ exchanger isoform of renal brush border.
1993,
Pubmed Chou,
Structural-functional correlation in chinchilla long loop of Henle thin limbs: a novel papillary subsegment.
1993,
Pubmed Greger,
Ion transport mechanisms in thick ascending limb of Henle's loop of mammalian nephron.
1985,
Pubmed Halm,
Apical membrane chloride channels in a colonic cell line activated by secretory agonists.
1988,
Pubmed Imai,
Dual effect of N-ethylmaleimide on Cl- transport across the thin ascending limb of Henle's loop.
1988,
Pubmed Imai,
Function of thin loops of Henle.
1987,
Pubmed Imai,
Transition of permeability properties along the descending limb of long-loop nephron.
1988,
Pubmed Imai,
Sodium chloride, urea, and water transport in the thin ascending limb of Henle. Generation of osmotic gradients by passive diffusion of solutes.
1974,
Pubmed Ishibashi,
Tissue expression of mRNA of chloride channel from MDCK cells and its regulation by protein kinases.
1993,
Pubmed Isozaki,
Selectivity of ion permeability across ascending thin limb of Henle's loop: interaction of Cl- and other halogens with anion transport system.
1991,
Pubmed Jentsch,
Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes.
1990,
Pubmed
,
Xenbase Kokko,
Countercurrent multiplication system without active transport in inner medulla.
1972,
Pubmed Kondo,
Effects of anion transport inhibitors and ion substitution on Cl- transport in TAL of Henle's loop.
1987,
Pubmed Kondo,
Effect of Ca2+ on Cl- transport in thin ascending limb of Henle's loop.
1988,
Pubmed Kondo,
Effect of pH on Cl- transport in TAL of Henle's loop.
1987,
Pubmed Krapivinsky,
Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln.
1994,
Pubmed
,
Xenbase Landry,
Molecular cloning and characterization of p64, a chloride channel protein from kidney microsomes.
1993,
Pubmed
,
Xenbase McCann,
Regulation of Cl- and K+ channels in airway epithelium.
1990,
Pubmed Paulmichl,
New mammalian chloride channel identified by expression cloning.
1992,
Pubmed
,
Xenbase Reeves,
Renal epithelial chloride channels.
1992,
Pubmed Riordan,
Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.
1989,
Pubmed Sasaki,
Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct.
1994,
Pubmed
,
Xenbase Steinmeyer,
Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel.
1991,
Pubmed
,
Xenbase Stephenson,
Concentration of urine in a central core model of the renal counterflow system.
1972,
Pubmed Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase Thornhill,
Biosynthesis of electroplax sodium channels in Electrophorus electrocytes and Xenopus oocytes.
1987,
Pubmed
,
Xenbase Uchida,
Expression of Madin-Darby canine kidney cell Na(+)-and Cl(-)-dependent taurine transporter in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase Uchida,
Molecular cloning of a chloride channel that is regulated by dehydration and expressed predominantly in kidney medulla.
1993,
Pubmed
,
Xenbase Yoshitomi,
Evidence for conductive Cl- pathways across the cell membranes of the thin ascending limb of Henle's loop.
1988,
Pubmed
