Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The alpha subunit of the human granulocyte-macrophage colony-stimulating factor receptor signals for glucose transport via a phosphorylation-independent pathway.
Ding DX
,
Rivas CI
,
Heaney ML
,
Raines MA
,
Vera JC
,
Golde DW
.
???displayArticle.abstract???
The receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF) is composed of an alpha and beta subunit, which together form the high-affinity receptor. The alpha subunit by itself binds ligand at low affinity, whereas the isolated beta subunit does not bind GM-CSF. It is generally believed that the high-affinity receptor is responsible for the multiple functions of GM-CSF and that the isolated alpha subunit (GMR alpha) does not transduce a signal. Xenopus laevis oocytes injected with RNA encoding human GMR alpha expressed up to 10(10) low-affinity sites for GM-CSF (Kd = 6 nM). GM-CSF binding to the alpha subunit expressed in Xenopus oocytes caused activation of 2-deoxyglucose transport through endogenous glucose transporters. 2-Deoxyglucose transport was stimulated by similar low concentrations of GM-CSF in HL-60 leukemia cells as well as normal human neutrophils and Xenopus oocytes expressing GMR alpha. Engagement of the isolated alpha subunit in oocytes did not lead to protein phosphorylation or tyrosine phosphorylation of mitogen-activated protein kinase (MAP kinase). Staurosporin and genistein inhibited GM-CSF-induced tyrosine phosphorylation of MAP kinase in human neutrophils and HL-60 cells without affecting GM-CSF-stimulated uptake of 2-deoxyglucose. These results provide direct evidence that the isolated alpha subunit signals for hexose transport and can do so without engagement of the kinase cascade. Our data also indicate that signaling for hexose uptake may occur in a phosphorylation-independent manner in cells expressing the high-affinity GM-CSF receptor.
Akiyama,
Genistein, a specific inhibitor of tyrosine-specific protein kinases.
1987, Pubmed
Akiyama,
Genistein, a specific inhibitor of tyrosine-specific protein kinases.
1987,
Pubmed
Baldwin,
Nonhematopoietic tumor cells express functional GM-CSF receptors.
1989,
Pubmed
Baldwin,
Identification and characterization of a low-affinity granulocyte-macrophage colony-stimulating factor receptor on primary and cultured human melanoma cells.
1991,
Pubmed
Birnbaum,
Identification of a novel gene encoding an insulin-responsive glucose transporter protein.
1989,
Pubmed
,
Xenbase
Budel,
Common binding structure for granulocyte macrophage colony-stimulating factor and interleukin-3 on human acute myeloid leukemia cells and monocytes.
1990,
Pubmed
Cannistra,
Differentiation-associated expression of two functionally distinct classes of granulocyte-macrophage colony-stimulating factor receptors by human myeloid cells.
1990,
Pubmed
Chiba,
Identification and cellular distribution of distinct proteins forming human GM-CSF receptor.
1990,
Pubmed
Chiba,
Characterization and molecular features of the cell surface receptor for human granulocyte-macrophage colony-stimulating factor.
1990,
Pubmed
Chiba,
Affinity purification of human granulocyte macrophage colony-stimulating factor receptor alpha-chain. Demonstration of binding by photoaffinity labeling.
1990,
Pubmed
Desbois,
Differential role of insulin receptor autophosphorylation sites 1162 and 1163 in the long-term insulin stimulation of glucose transport, glycogenesis, and protein synthesis.
1992,
Pubmed
DiPersio,
Characterization of the human granulocyte-macrophage colony-stimulating factor receptor.
1988,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Duronio,
Tyrosine phosphorylation of receptor beta subunits and common substrates in response to interleukin-3 and granulocyte-macrophage colony-stimulating factor.
1992,
Pubmed
Eder,
The human granulocyte-macrophage colony-stimulating factor receptor is capable of initiating signal transduction in NIH3T3 cells.
1993,
Pubmed
Elliott,
Recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor show common biological effects and binding characteristics on human monocytes.
1989,
Pubmed
Forsayeth,
Monoclonal antibodies to the human insulin receptor that activate glucose transport but not insulin receptor kinase activity.
1987,
Pubmed
Gasson,
High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells.
1986,
Pubmed
Gearing,
Expression cloning of a receptor for human granulocyte-macrophage colony-stimulating factor.
1989,
Pubmed
Gomez-Cambronero,
Granulocyte-macrophage colony-stimulating factor-induced protein tyrosine phosphorylation of microtubule-associated protein kinase in human neutrophils.
1992,
Pubmed
Hamilton,
Activation and proliferation signals in murine macrophages: stimulation of glucose uptake by hemopoietic growth factors and other agents.
1988,
Pubmed
Hayashida,
Molecular cloning of a second subunit of the receptor for human granulocyte-macrophage colony-stimulating factor (GM-CSF): reconstitution of a high-affinity GM-CSF receptor.
1990,
Pubmed
Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Kanakura,
Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce rapid phosphorylation and activation of the proto-oncogene Raf-1 in a human factor-dependent myeloid cell line.
1991,
Pubmed
Kanakura,
Signal transduction of the human granulocyte-macrophage colony-stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins.
1990,
Pubmed
Kitamura,
Expression cloning of the human IL-3 receptor cDNA reveals a shared beta subunit for the human IL-3 and GM-CSF receptors.
1991,
Pubmed
Linnekin,
Signal transduction of human interleukin 3 and granulocyte-macrophage colony-stimulating factor through serine and tyrosine phosphorylation.
1990,
Pubmed
Lopez,
Human interleukin-3 inhibits the binding of granulocyte-macrophage colony-stimulating factor and interleukin-5 to basophils and strongly enhances their functional activity.
1990,
Pubmed
Metcalf,
Low-affinity placenta-derived receptors for human granulocyte-macrophage colony-stimulating factor can deliver a proliferative signal to murine hemopoietic cells.
1990,
Pubmed
Miyazawa,
Comparative analysis of signaling pathways between mast cell growth factor (c-kit ligand) and granulocyte-macrophage colony-stimulating factor in a human factor-dependent myeloid cell line involves phosphorylation of Raf-1, GTPase-activating protein and mitogen-activated protein kinase.
1991,
Pubmed
Moller,
Biologic activities of naturally occurring human insulin receptor mutations. Evidence that metabolic effects of insulin can be mediated by a kinase-deficient insulin receptor mutant.
1991,
Pubmed
Okuda,
Granulocyte-macrophage colony-stimulating factor, interleukin-3, and steel factor induce rapid tyrosine phosphorylation of p42 and p44 MAP kinase.
1992,
Pubmed
Opresko,
Functional reconstitutional of the human epidermal growth factor receptor system in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Park,
Characterization of the cell surface receptor for human granulocyte/macrophage colony-stimulating factor.
1986,
Pubmed
Park,
Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor.
1989,
Pubmed
Park,
Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.
1992,
Pubmed
Pessin,
Mammalian facilitative glucose transporter family: structure and molecular regulation.
1992,
Pubmed
Polotskaya,
A critical role for the cytoplasmic domain of the granulocyte-macrophage colony-stimulating factor alpha receptor in mediating cell growth.
1993,
Pubmed
Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Quelle,
Interleukin 3, granulocyte-macrophage colony-stimulating factor, and transfected erythropoietin receptors mediate tyrosine phosphorylation of a common cytosolic protein (pp100) in FDC-ER cells.
1992,
Pubmed
Raines,
Granulocyte-macrophage colony-stimulating factor activates microtubule-associated protein 2 kinase in neutrophils via a tyrosine kinase-dependent pathway.
1992,
Pubmed
Raines,
Identification and molecular cloning of a soluble human granulocyte-macrophage colony-stimulating factor receptor.
1991,
Pubmed
Roth,
Substrates and signalling complexes: the tortured path to insulin action.
1992,
Pubmed
Sakamaki,
Critical cytoplasmic domains of the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors for growth signal transduction and tyrosine phosphorylation.
1992,
Pubmed
Satoh,
Involvement of ras p21 protein in signal-transduction pathways from interleukin 2, interleukin 3, and granulocyte/macrophage colony-stimulating factor, but not from interleukin 4.
1991,
Pubmed
Sung,
Insulin receptor signaling through non-tyrosine kinase pathways: evidence from anti-receptor antibodies and insulin receptor mutants.
1992,
Pubmed
Taketazu,
IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor.
1991,
Pubmed
Tavernier,
A human high affinity interleukin-5 receptor (IL5R) is composed of an IL5-specific alpha chain and a beta chain shared with the receptor for GM-CSF.
1991,
Pubmed
Vera,
Reconstitution of an insulin signaling pathway in Xenopus laevis oocytes: coexpression of a mammalian insulin receptor and three different mammalian hexose transporters.
1990,
Pubmed
,
Xenbase
Whetton,
Haemopoietic cell growth factor mediates cell survival via its action on glucose transport.
1984,
Pubmed
Williams,
Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosis.
1990,
Pubmed
