Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Point mutations 5' to the tRNA selenocysteine TATA box alter RNA polymerase III transcription by affecting the binding of TBP.
Myslinski E, Schuster C, Huet J, Sentenac A, Krol A, Carbon P.
???displayArticle.abstract???
The selenocysteine tRNA(Sec) gene possesses two external promoter elements, one of which is constituted by a strong TATA box. Point mutant analysis performed in this study led to the conclusion that the functional TATA promoter actually encompasses the sequence -34 GGGTATAAAAGG-23. Individual changes at T-31 do not affect transcription much. Position T-29 is less permissive to mutation since transversion to a G, for example, is less well tolerated than at T-31. Interestingly, a double point mutation, converting GG(-33/-32) to TT, causes abrogation of transcription in vivo and severe reduction of transcription in vitro with human TBP. Therefore, data obtained underscore the fact that, in the Xenopus tRNA(Sec), these two Gs are an integral part of the TATA promoter. Gel retardation experiments indicate that the GG to TT substitution, which led human TBP to lose its ability to support efficient transcription in vitro, correlates with the appearance of an altered pattern of retarded complexes. Altogether, the data presented in this report support a model in which TBP interacts directly with the TATA element of the tRNA(Sec) gene, in contrast to the type of interaction proposed for classical TATA-less tRNA genes.
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988, Pubmed,
Xenbase
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase Burnol,
TFIIIC relieves repression of U6 snRNA transcription by chromatin.
1993,
Pubmed
,
Xenbase Burton,
Expression in Escherichia coli: purification and properties of the yeast general transcription factor TFIID.
1991,
Pubmed Carbon,
Transcription of the Xenopus laevis selenocysteine tRNA(Ser)Sec gene: a system that combines an internal B box and upstream elements also found in U6 snRNA genes.
1991,
Pubmed
,
Xenbase Carbon,
A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
1987,
Pubmed
,
Xenbase Gabrielsen,
RNA polymerase III (C) and its transcription factors.
1991,
Pubmed
,
Xenbase Hernandez,
TBP, a universal eukaryotic transcription factor?
1993,
Pubmed Huet,
The TATA-binding protein participates in TFIIIB assembly on tRNA genes.
1992,
Pubmed Kassavetis,
The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB.
1992,
Pubmed Kim,
Crystal structure of a yeast TBP/TATA-box complex.
1993,
Pubmed Kim,
Co-crystal structure of TBP recognizing the minor groove of a TATA element.
1993,
Pubmed Kunkel,
RNA polymerase III transcription of genes that lack internal control regions.
1991,
Pubmed
,
Xenbase Lee,
Selenocysteine tRNA[Ser]Sec gene is ubiquitous within the animal kingdom.
1990,
Pubmed
,
Xenbase Lee,
TFIIA induces conformational changes in TFIID via interactions with the basic repeat.
1992,
Pubmed Lee,
Interaction of TFIID in the minor groove of the TATA element.
1991,
Pubmed Lee,
Transcription of Xenopus selenocysteine tRNA Ser (formerly designated opal suppressor phosphoserine tRNA) gene is directed by multiple 5'-extragenic regulatory elements.
1989,
Pubmed
,
Xenbase Lescure,
The proximal promoter and the start site cooperate to specify correct U1 snRNA transcription initiation by RNA polymerase II.
1992,
Pubmed
,
Xenbase Lobo,
The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.
1991,
Pubmed Lobo,
A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.
1992,
Pubmed Margottin,
Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C.
1991,
Pubmed Myslinski,
Optimal tRNA((Ser)Sec) gene activity requires an upstream SPH motif.
1992,
Pubmed
,
Xenbase Myslinski,
Promoter strength and structure dictate module composition in RNA polymerase III transcriptional activator elements.
1993,
Pubmed
,
Xenbase Pugh,
Diverse transcriptional functions of the multisubunit eukaryotic TFIID complex.
1992,
Pubmed Ruet,
Isolation of a class C transcription factor which forms a stable complex with tRNA genes.
1984,
Pubmed Sharp,
TATA-binding protein is a classless factor.
1992,
Pubmed Simmen,
Cofractionation of the TATA-binding protein with the RNA polymerase III transcription factor TFIIIB.
1992,
Pubmed Simmen,
TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III.
1991,
Pubmed Sprague,
New twists in class III transcription.
1992,
Pubmed Starr,
TFIID binds in the minor groove of the TATA box.
1991,
Pubmed Sturchler,
Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA(Sec).
1993,
Pubmed
,
Xenbase Taggart,
The TATA-binding protein and associated factors are components of pol III transcription factor TFIIIB.
1992,
Pubmed Waldschmidt,
Identification of transcription factors required for the expression of mammalian U6 genes in vitro.
1991,
Pubmed White,
Mechanism of TATA-binding protein recruitment to a TATA-less class III promoter.
1992,
Pubmed Wobbe,
Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro.
1990,
Pubmed
