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EMBO J
1993 Jun 01;126:2549-58. doi: 10.1002/j.1460-2075.1993.tb05910.x.
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A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains is required for ribosomal RNA processing.
Jansen R, Tollervey D, Hurt EC.
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Yeast fibrillarin (NOP1) is an evolutionarily conserved, nucleolar protein necessary for multiple steps in ribosome biogenesis. Yeast mutants lacking a functional NOP1 gene can be complemented by human fibrillarin but are temperature sensitive for growth and impaired in pre-rRNA processing. In order to identify components which interact functionally with human fibrillarin in yeast, we isolated extragenic suppressors of this phenotype. One dominant suppressor, sof1-56, which is allele-specific for human fibrillarin and restores growth and pre-RNA processing at 35 degrees C, was cloned by in vivo complementation. The wild-type allele of SOF1 is essential for cell growth and encodes a novel 56 kDa protein. In its central domain, SOF1 contains a repeated sequence also found in beta-subunits of trimeric G-proteins and the splicing factor PRP4. A single amino acid exchange in the G beta-like repeat domain is responsible for the suppressing activity of sof1-56. Indirect immunofluorescence shows that SOF1 is located within the yeast nucleolus. Co-immunoprecipitation demonstrates the physical association of SOF1 with U3 small nucleolar RNA and NOP1. In vivo depletion of SOF1 leads to impaired pre-rRNA processing and inhibition of 18S rRNA production. Thus, SOF1 is a new component of the nucleolar rRNA processing machinery.
Aris,
cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera.
1991, Pubmed,
Xenbase
Aris,
cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera.
1991,
Pubmed
,
Xenbase Banroques,
PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.
1989,
Pubmed Baserga,
Distinct molecular signals for nuclear import of the nucleolar snRNA, U3.
1992,
Pubmed
,
Xenbase Beltrame,
Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.
1992,
Pubmed
,
Xenbase Berben,
The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae.
1991,
Pubmed Blumer,
Receptor-G protein signaling in yeast.
1991,
Pubmed Bordonné,
Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo.
1990,
Pubmed Carmo-Fonseca,
Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.
1991,
Pubmed Clark,
SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs.
1990,
Pubmed Dalrymple,
The product of the PRP4 gene of S. cerevisiae shows homology to beta subunits of G proteins.
1989,
Pubmed Deng,
COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain.
1992,
Pubmed Duronio,
Comparative analysis of the beta transducin family with identification of several new members including PWP1, a nonessential gene of Saccharomyces cerevisiae that is divergently transcribed from NMT1.
1992,
Pubmed Elledge,
A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae.
1988,
Pubmed Fischer,
Assigning functions to nucleolar structures.
1991,
Pubmed Fong,
Distinct forms of the beta subunit of GTP-binding regulatory proteins identified by molecular cloning.
1987,
Pubmed Fong,
Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs.
1986,
Pubmed Girard,
GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast.
1992,
Pubmed Guarente,
A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
1982,
Pubmed Hartley,
A deduced gene product from the Drosophila neurogenic locus, enhancer of split, shows homology to mammalian G-protein beta subunit.
1988,
Pubmed Henríquez,
Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen.
1990,
Pubmed Hereford,
Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis.
1974,
Pubmed Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed Hurt,
Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae.
1988,
Pubmed Ito,
Transformation of intact yeast cells treated with alkali cations.
1983,
Pubmed Jansen,
Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.
1991,
Pubmed
,
Xenbase Kass,
The first pre-rRNA-processing event occurs in a large complex: analysis by gel retardation, sedimentation, and UV cross-linking.
1990,
Pubmed Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed Klinkert,
Expression of diagnostic 31/32 kilodalton proteins of Schistosoma mansoni as fusions with bacteriophage MS2 polymerase.
1988,
Pubmed Lapeyre,
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
1990,
Pubmed
,
Xenbase Last,
Evidence for related functions of the RNA genes of Saccharomyces cerevisiae.
1987,
Pubmed Lawrence,
Classical mutagenesis techniques.
1991,
Pubmed Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed Lustig,
The yeast RNA gene products are essential for mRNA splicing in vitro.
1986,
Pubmed Maser,
U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.
1989,
Pubmed Myslinski,
An intron in the genes for U3 small nucleolar RNAs of the yeast Saccharomyces cerevisiae.
1990,
Pubmed Ochs,
Fibrillarin: a new protein of the nucleolus identified by autoimmune sera.
1985,
Pubmed
,
Xenbase Parker,
Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
1987,
Pubmed Rose,
Cloning genes by complementation in yeast.
1991,
Pubmed Rothman,
Molecular dissection of the secretory pathway.
1992,
Pubmed Rothstein,
One-step gene disruption in yeast.
1983,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Scheer,
Functional and dynamic aspects of the mammalian nucleolus.
1990,
Pubmed Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed Strathern,
Recovery of plasmids from yeast into Escherichia coli: shuttle vectors.
1991,
Pubmed Tollervey,
The role of small nucleolar ribonucleoproteins in ribosome synthesis.
1990,
Pubmed Tollervey,
A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
1987,
Pubmed Tollervey,
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
1991,
Pubmed Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed Tyc,
A new interaction between the mouse 5' external transcribed spacer of pre-rRNA and U3 snRNA detected by psoralen crosslinking.
1992,
Pubmed Warner,
The nucleolus and ribosome formation.
1990,
Pubmed Whiteway,
The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein.
1989,
Pubmed Williams,
Characterization of TUP1, a mediator of glucose repression in Saccharomyces cerevisiae.
1990,
Pubmed Wimmer,
A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.
1992,
Pubmed
