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Mol Cell Biol
1992 Aug 01;128:3548-55. doi: 10.1128/mcb.12.8.3548-3555.1992.
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Comparative analysis of the intracellular localization of c-Myc, c-Fos, and replicative proteins during cell cycle progression.
Vriz S, Lemaitre JM, Leibovici M, Thierry N, Méchali M.
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In eukaryotic cells, nucleus-cytoplasm exchanges play an important role in genomic regulation. We have analyzed the localization of four nuclear antigens in different growth conditions: two replicative proteins, DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), and two oncogenic regulatory proteins, c-Myc and c-Fos. A kinetic study of subcellular localization of these proteins has been done. In cultures in which cells were sparse, these proteins were detected in the nucleus. When proliferation was stopped by the high density of culture cells or by serum starvation, these proteins left the nucleus for the cytoplasm with different kinetics. DNA polymerase alpha is the first protein to leave the nucleus, with the PCNA protein, c-Fos, and c-Myc leaving the nucleus later. In contrast, during serum stimulation c-Fos and c-Myc relocalize into the nucleus before the replicative proteins. We also noticed that in sparse cell cultures, 10% of the cells exhibit a perinuclear staining for the DNA polymerase alpha, PCNA, and c-Myc proteins but not for c-Fos. This peculiar staining was also observed as an initial step to nuclear localization after serum stimulation and in vivo in Xenopus embryos when the G1 phase is reintroduced in the embryonic cell cycle at the mid-blastula stage. We suggest that such staining could reflect specific structures involved in the initiation of the S phase.
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Blow,
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Pubmed
,
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Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.
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Changes in the nuclear distribution of cyclin (PCNA) during S-phase are not triggered by post-translational modifications that are expected to moderately affect its charge.
1985,
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The myc oncogene: its role in transformation and differentiation.
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Pubmed Curran,
Viral and cellular fos proteins: a comparative analysis.
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Pubmed Dent,
A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus.
1989,
Pubmed
,
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Transport across the nuclear envelope: enigmas and explanations.
1991,
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The permeability of the nuclear envelope in dividing and nondividing cell cultures.
1990,
Pubmed
,
Xenbase Gerace,
Functional organization of the nuclear envelope.
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Pubmed Gusse,
Translocation of a store of maternal cytoplasmic c-myc protein into nuclei during early development.
1989,
Pubmed
,
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Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells.
1984,
Pubmed Hunt,
Cytoplasmic anchoring proteins and the control of nuclear localization.
1989,
Pubmed Hutchison,
Changes in the nuclear distribution of DNA polymerase alpha and PCNA/cyclin during the progress of the cell cycle, in a cell-free extract of Xenopus eggs.
1989,
Pubmed
,
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Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein.
,
Pubmed Lüscher,
New light on Myc and Myb. Part I. Myc.
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Pubmed Moore,
A sensitive and quantitative enzyme-linked immunosorbence assay for the c-myc and N-myc oncoproteins.
1987,
Pubmed Müller,
Induction of c-fos gene and protein by growth factors precedes activation of c-myc.
,
Pubmed Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Nigg,
Nuclear import-export: in search of signals and mechanisms.
1991,
Pubmed Pardee,
G1 events and regulation of cell proliferation.
1989,
Pubmed Rabbitts,
Metabolism of c-myc gene products: c-myc mRNA and protein expression in the cell cycle.
1985,
Pubmed Roth,
A gradient of nuclear localization of the dorsal protein determines dorsoventral pattern in the Drosophila embryo.
1989,
Pubmed Roux,
Nuclear localization of c-Fos, but not v-Fos proteins, is controlled by extracellular signals.
1990,
Pubmed Rushlow,
The graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila.
1989,
Pubmed Sadaie,
Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (PCNA) in HeLa cells.
1986,
Pubmed Shyy,
Effect of growth state and heat shock on nucleolar localization of the 110,000-Da heat shock protein in mouse embryo fibroblasts.
1986,
Pubmed Sorger,
Cloning and expression of a gene encoding hsc73, the major hsp70-like protein in unstressed rat cells.
1987,
Pubmed Steward,
Relocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function.
1989,
Pubmed Tanaka,
Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha.
1982,
Pubmed Taylor,
Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.
1986,
Pubmed
,
Xenbase Verrier,
Wounding a fibroblast monolayer results in the rapid induction of the c-fos proto-oncogene.
1986,
Pubmed Wahl,
Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle.
1988,
Pubmed Wang,
Eukaryotic DNA polymerases.
1991,
Pubmed
