Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1991 Nov 01;1111:5410-6. doi: 10.1128/mcb.11.11.5410-5416.1991.
Show Gene links
Show Anatomy links
Conserved mechanism of tRNA splicing in eukaryotes.
Zillmann M, Gorovsky MA, Phizicky EM.
???displayArticle.abstract???
The ligation steps of tRNA splicing in yeast and vertebrate cells have been thought to proceed by fundamentally different mechanisms. Ligation in yeast cells occurs by incorporation of an exogenous phosphate from ATP into the splice junction, with concomitant formation of a 2' phosphate at the 5' junction nucleotide. This phosphate is removed in a subsequent step which, in vitro, is catalyzed by an NAD-dependent dephosphorylating activity. In contrast, tRNA ligation in vertebrates has been reported to occur without incorporation of exogenous phosphate or formation of a 2' phosphate. We demonstrate in this study the existence of a yeast tRNA ligase-like activity in HeLa cells. Furthermore, in extracts from these cells, the entire yeastlike tRNA splicing machinery is intact, including that for cleavage, ligation, and removal of the 2' phosphate in an NAD-dependent fashion to give mature tRNA. These results argue that the mechanism of tRNA splicing is conserved among eukaryotes.
Apostol,
Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.
1991, Pubmed
Apostol,
Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.
1991,
Pubmed Attardi,
Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease.
1985,
Pubmed
,
Xenbase Baldi,
Binding and cleavage of pre-tRNA by the Xenopus splicing endonuclease: two separable steps of the intron excision reaction.
1986,
Pubmed
,
Xenbase Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed Filipowicz,
Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
1983,
Pubmed
,
Xenbase Filipowicz,
RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.
1983,
Pubmed
,
Xenbase Green,
The discovery of new intron-containing human tRNA genes using the polymerase chain reaction.
1990,
Pubmed Greer,
Substrate recognition and identification of splice sites by the tRNA-splicing endonuclease and ligase from Saccharomyces cerevisiae.
1987,
Pubmed Greer,
Mechanism of action of a yeast RNA ligase in tRNA splicing.
1983,
Pubmed Kikuchi,
Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.
1982,
Pubmed Konarska,
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.
1982,
Pubmed Konarska,
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
,
Pubmed Laski,
Characterization of tRNA precursor splicing in mammalian extracts.
1983,
Pubmed
,
Xenbase Lee,
Transfer RNA splicing in Saccharomyces cerevisiae. Secondary and tertiary structures of the substrates.
1985,
Pubmed Mattoccia,
Site selection by the tRNA splicing endonuclease of Xenopus laevis.
1988,
Pubmed
,
Xenbase McCraith,
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.
1991,
Pubmed McCraith,
A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.
1990,
Pubmed Nishikura,
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.
1981,
Pubmed
,
Xenbase Peebles,
Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.
1983,
Pubmed Perkins,
Isolation and characterization of an RNA ligase from HeLa cells.
1985,
Pubmed Phizicky,
Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene.
1986,
Pubmed Pick,
Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity.
1986,
Pubmed Pick,
Purification of wheat germ RNA ligase. II. Mechanism of action of wheat germ RNA ligase.
1986,
Pubmed Reyes,
Substrate recognition and splice site determination in yeast tRNA splicing.
1988,
Pubmed Schwartz,
Enzymatic mechanism of an RNA ligase from wheat germ.
1983,
Pubmed Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Stange,
Wheat germ splicing endonuclease is highly specific for plant pre-tRNAs.
1988,
Pubmed
,
Xenbase Swerdlow,
Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.
1984,
Pubmed Szekely,
Intron sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.
1988,
Pubmed Tyc,
Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.
1983,
Pubmed van Tol,
All human tRNATyr genes contain introns as a prerequisite for pseudouridine biosynthesis in the anticodon.
1988,
Pubmed Winicov,
Nuclear ligation of RNA 5'-OH kinase products in tRNA.
1982,
Pubmed
