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???displayArticle.abstract??? Maturation-promoting factor (MPF) is a cell cycle control element able to cause cells to enter M-phase upon microinjection and will induce metaphase in nuclei incubated in cell extracts. Previous work has shown that MPF is composed of a complex between p34cdc 2 protein kinase and a B-type cyclin. In the present work gamma-S-ATP was found to cause activation of MPF activity in partially purified preparations, but this activation was lost upon chromatography on Matrex Green gel A. Readdition of other Matrex Green fractions to purified MPF restored the ability of gamma-S-ATP to activate MPF for nuclear breakdown as well as phosphorylation of histone H1. Use of the system described here will facilitate study of p34cdc 2 kinase activation and identification of elements involved in MPF regulation.
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988, Pubmed,
Xenbase
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase Booher,
Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules.
1988,
Pubmed Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
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Xenbase Cyert,
Monoclonal antibodies specific for thiophosphorylated proteins recognize Xenopus MPF.
1988,
Pubmed
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Xenbase Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
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Xenbase Dunphy,
Mitosis-inducing factors are present in a latent form during interphase in the Xenopus embryo.
1988,
Pubmed
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Xenbase Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase Erikson,
Biochemical characterization of the p34cdc2 protein kinase component of purified maturation-promoting factor from Xenopus eggs.
1989,
Pubmed
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Xenbase Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
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Xenbase Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
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Xenbase Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
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Xenbase Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed Hayles,
The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function.
1986,
Pubmed Kishimoto,
Generality of the action of various maturation-promoting factors.
1982,
Pubmed
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Xenbase Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
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Xenbase Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed Langan,
Mammalian growth-associated H1 histone kinase: a homolog of cdc2+/CDC28 protein kinases controlling mitotic entry in yeast and frog cells.
1989,
Pubmed
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Xenbase Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
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Pubmed Lee,
Cell cycle control genes in fission yeast and mammalian cells.
1988,
Pubmed Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
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Xenbase Lohka,
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1985,
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Xenbase Lohka,
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Xenbase Masui,
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1971,
Pubmed Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
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Xenbase Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
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Xenbase Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
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Xenbase NULL,
Cyclin in fission yeast.
1988,
Pubmed Pines,
Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
1987,
Pubmed
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Xenbase Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed Russell,
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.
1987,
Pubmed Russell,
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1987,
Pubmed Simanis,
The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.
1986,
Pubmed Smith,
The interaction of steroids with Rana pipiens Oocytes in the induction of maturation.
1971,
Pubmed Wittenberg,
Control of the yeast cell cycle is associated with assembly/disassembly of the Cdc28 protein kinase complex.
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