Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Minichromosome assembly accompanying repair-type DNA synthesis in Xenopus oocytes.
Ryoji M, Tominna E, Yasui W.
???displayArticle.abstract???
Minichromosomes were assembled by injection of circular DNA into the nucleus of Xenopus oocytes. We observed that, in the course of DNA supercoiling and chromatin assembly, a small percentage of the injected DNA molecules incorporated a radioactive precursor. This DNA synthesis was carried out by aphidicolin-sensitive DNA polymerase, and generated short repair-like patches covalently linked to the injected DNA. We found that the DNA thus repaired was rapidly supercoiled almost to completion within 15 to 30 min after injection, whereas 60 to 120 min were required to supercoil the intact, bulk DNA molecules. Such differential supercoiling kinetics was also observed when UV-damaged DNA was injected. Chromatin assembly, which was characterized by DNA fragment sizes protected from micrococcal nuclease digestion, was consistent with the rapid DNA supercoiling and proceeded more efficiently on the repaired DNA. These results indicate that there are at least two kinetically distinct ways of assembling minichromosomes in the oocytenucleus, and that the repaired DNA molecules preferentially follow the faster pathway.
???displayArticle.pubmedLink???
2513559 ???displayArticle.pmcLink???PMC335298 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988, Pubmed,
Xenbase
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase Almouzni,
Assembly of spaced chromatin involvement of ATP and DNA topoisomerase activity.
1988,
Pubmed
,
Xenbase Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase Cusick,
Structure of chromatin at deoxyribonucleic acid replication forks: prenucleosomal deoxyribonucleic acid is rapidly excised from replicating simian virus 40 chromosomes by micrococcal nuclease.
1981,
Pubmed Denhardt,
Comparison of the effect of ultraviolet radiation and ethidium bromide intercalation on the conformation of superhelical phiX174 replicative form DNA.
1973,
Pubmed Dresler,
Involvement of DNA polymerase delta in DNA repair synthesis in human fibroblasts at late times after ultraviolet irradiation.
1988,
Pubmed Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase Ford,
DNA synthesis in ocytes and eggs of Xenopus laevis injected with DNA.
1975,
Pubmed
,
Xenbase Gargiulo,
Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes.
1983,
Pubmed
,
Xenbase Germond,
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
1975,
Pubmed Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase Hammond,
Identification of DNA polymerase delta in CV-1 cells: studies implicating both DNA polymerase delta and DNA polymerase alpha in DNA replication.
1987,
Pubmed Hanaoka,
Aphidicolin does inhibit repair replication in HeLa cells.
1979,
Pubmed Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Hunting,
Multiple conformational states of repair patches in chromatin during DNA excision repair.
1985,
Pubmed Ikegami,
Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha.
1978,
Pubmed Kaiserman,
Characterization of a stable, major DNA polymerase alpha species devoid of DNA primase activity.
1987,
Pubmed
,
Xenbase Kornberg,
DNA replication.
1988,
Pubmed Lacks,
A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.
1975,
Pubmed Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase Lee,
Differential effects of dimethylsulfoxide on the activities of human DNA polymerases alpha and delta.
1986,
Pubmed Legerski,
Repair of UV-induced lesions in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase Legerski,
A sensitive endonuclease probe for lesions in deoxyribonucleic acid helix structure produced by carcinogenic or mutagenic agents.
1977,
Pubmed McDonell,
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
1977,
Pubmed Mertz,
Linear DNA does not form chromatin containing regularly spaced nucleosomes.
1982,
Pubmed
,
Xenbase Mullinger,
Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair.
1987,
Pubmed Nishida,
DNA repair synthesis in human fibroblasts requires DNA polymerase delta.
1988,
Pubmed Noll,
Self-assembly of single and closely spaced nucleosome core particles.
1980,
Pubmed Oudet,
Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
1975,
Pubmed Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Ruiz-Carrillo,
In vitro core particle and nucleosome assembly at physiological ionic strength.
1979,
Pubmed Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase Ryoji,
Structure of the two distinct types of minichromosomes that are assembled on DNA injected in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Smith,
Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro.
1989,
Pubmed So,
Mammalian DNA polymerases alpha and delta: current status in DNA replication.
1988,
Pubmed Steinmetz,
Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin.
1978,
Pubmed Stillman,
Chromatin assembly during SV40 DNA replication in vitro.
1986,
Pubmed Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed Watkins,
Nucleosome rearrangement in vitro. 2. Formation of nucleosomes in newly repaired regions of DNA.
1985,
Pubmed Wobbe,
In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.
1985,
Pubmed Worcel,
Assembly of newly replicated chromatin.
1978,
Pubmed Yasui,
Characterization of early DNA synthesis in Xenopus eggs after injection of circular plasmid DNA.
1989,
Pubmed
,
Xenbase Zierler,
Stockpiling of DNA polymerases during oogenesis and embryogenesis in the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase Zuber,
Involvement of proliferating cell nuclear antigen (cyclin) in DNA replication in living cells.
1989,
Pubmed
,
Xenbase
