Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Tissue-specific processing and polarized compartmentalization of clone-produced cholinesterase in microinjected Xenopus oocytes.
Dreyfus PA, Seidman S, Pincon-Raymond M, Murawsky M, Rieger F, Schejter E, Zakut H, Soreq H.
???displayArticle.abstract???
1. To approach the involvement of tissue-specific elements in the compartmentalization of ubiquitous polymorphic proteins, immunohistochemical methods were used to analyze the localization of butyrylcholinesterase (BuChE) in Xenopus oocytes microinjected with synthetic BuChEmRNA alone and in combination with tissue-extracted mRNAs. 2. When injected alone BuChEmRNA efficiently directed the synthesis of small membrane-associated accumulations localized principally on the external surface of the oocyte's animal pole. Tunicamycin blocked the appearance of such accumulations, suggesting that glycosylation is involved in the transport of nascent BuChE molecules to the oocyte's surface. Coinjection with brain or muscle mRNA, but not liver mRNA, facilitated the formation of pronounced, tissue-characteristic BuChE aggregates. 3. These findings implicate tissue-specific mRNAs in the assembly of the clone-produced protein and in its nonuniform distribution in the oocyte membrane or extracellular material.
Anglister,
Basal lamina directs acetylcholinesterase accumulation at synaptic sites in regenerating muscle.
1985, Pubmed
Anglister,
Basal lamina directs acetylcholinesterase accumulation at synaptic sites in regenerating muscle.
1985,
Pubmed Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase Brandan,
The synaptic form of acetylcholinesterase binds to cell-surface heparan sulfate proteoglycans.
1986,
Pubmed Brimijoin,
Immunology and molecular biology of the cholinesterases: current results and prospects.
1986,
Pubmed Danilchik,
Differentiation of the animal-vegetal axis in Xenopus laevis oocytes. I. Polarized intracellular translocation of platelets establishes the yolk gradient.
1987,
Pubmed
,
Xenbase Dascal,
Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Dreyfus,
Acetylcholinesterase of mammalian neuromuscular junctions: presence of tailed asymmetric acetylcholinesterase in synaptic basal lamina and sarcolemma.
1983,
Pubmed Dreyfus,
Cross-homologies and structural differences between human cholinesterases revealed by antibodies against cDNA-produced human butyrylcholinesterase peptides.
1988,
Pubmed Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase ELLMAN,
A new and rapid colorimetric determination of acetylcholinesterase activity.
1961,
Pubmed Fuller,
An enzymatic assay reveals that proteins destined for the apical or basolateral domains of an epithelial cell line share the same late Golgi compartments.
1985,
Pubmed Gibney,
Divergence in primary structure between the molecular forms of acetylcholinesterase.
1988,
Pubmed Hall,
Multiple forms of acetylcholinesterase and their distribution in endplate and non-endplate regions of rat diaphragm muscle.
1973,
Pubmed Houamed,
Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA.
,
Pubmed
,
Xenbase Inestrosa,
A membrane-associated dimer of acetylcholinesterase from Xenopus skeletal muscle is solubilized by phosphatidylinositol-specific phospholipase C.
1988,
Pubmed
,
Xenbase Inestrosa,
Acetylcholinesterase from bovine caudate nucleus is attached to membranes by a novel subunit distinct from those of acetylcholinesterases in other tissues.
1987,
Pubmed Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase Lockridge,
Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes.
1986,
Pubmed Lockridge,
Complete amino acid sequence of human serum cholinesterase.
1987,
Pubmed Massoulié,
The molecular forms of cholinesterase and acetylcholinesterase in vertebrates.
1982,
Pubmed Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase McMahan,
Cholinesterase is associated with the basal lamina at the neuromuscular junction.
1978,
Pubmed Mishina,
Expression of functional acetylcholine receptor from cloned cDNAs.
,
Pubmed
,
Xenbase Møllgård,
Synthesis and localization of plasma proteins in the developing human brain. Integrity of the fetal blood-brain barrier to endogenous proteins of hepatic origin.
1988,
Pubmed
,
Xenbase Opresko,
Specific proteolysis regulates fusion between endocytic compartments in Xenopus oocytes.
1987,
Pubmed
,
Xenbase Palecek,
Dynamics of tubulin structures in Xenopus laevis oogenesis.
1985,
Pubmed
,
Xenbase Prody,
Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues.
1987,
Pubmed Roberts,
Differences in the glycolipid membrane anchors of bovine and human erythrocyte acetylcholinesterases.
1987,
Pubmed Rosenberry,
Quantitative simulation of endplate currents at neuromuscular junctions based on the reaction of acetylcholine with acetylcholine receptor and acetylcholinesterase.
1979,
Pubmed Salpeter,
Electron microscope radioautography as a quantitative tool in enzyme cytochemistry. I. The distribution of acetylcholinesterase at motor end plates of a vertebrate twitch muscle.
1967,
Pubmed Sikorav,
Complex alternative splicing of acetylcholinesterase transcripts in Torpedo electric organ; primary structure of the precursor of the glycolipid-anchored dimeric form.
1988,
Pubmed Sikorav,
cDNA sequences of Torpedo marmorata acetylcholinesterase: primary structure of the precursor of a catalytic subunit; existence of multiple 5'-untranslated regions.
1987,
Pubmed Silman,
Modes of attachment of acetylcholinesterase to the surface membrane.
1987,
Pubmed Sørensen,
An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties.
1982,
Pubmed Soreq,
Expression of cholinesterase gene(s) in human brain tissues: translational evidence for multiple mRNA species.
1984,
Pubmed
,
Xenbase Soreq,
The use of mRNA translation in vitro and in ovo followed by crossed immunoelectrophoretic autoradiography to study the biosynthesis of human cholinesterases.
1986,
Pubmed
,
Xenbase Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase Soreq,
The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes.
1985,
Pubmed
,
Xenbase Soreq,
Expression of cholinesterase genes in human oocytes revealed by in-situ hybridization.
1987,
Pubmed Sytkowski,
Development of acetylcholine receptor clusters on cultured muscle cells.
1973,
Pubmed Wallace,
Aggregating factor from Torpedo electric organ induces patches containing acetylcholine receptors, acetylcholinesterase, and butyrylcholinesterase on cultured myotubes.
1986,
Pubmed Wallace,
Aggregates of acetylcholinesterase induced by acetylcholine receptor-aggregating factor.
,
Pubmed Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase Wischnitzer,
The ultrastructure of the cytoplasm of the developing amphibian egg.
1966,
Pubmed Zakut,
Polymorphism of acetylcholinesterase in discrete regions of the developing human fetal brain.
1985,
Pubmed
,
Xenbase
