Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Changes in DNA topology can modulate in vitro transcription of certain RNA polymerase III genes.
Sekiguchi JM, Swank RA, Kmiec EB.
???displayArticle.abstract???
The role of DNA supercoiling in eukaryotic gene expression is not fully understood. The objective of this study was to examine the regulation of in vitro chromatin assembly by topological alterations in the DNA template using a cell-free extract from Xenopus laevis oocytes (S-150). The results suggest that input DNA topology may be a determining factor in controlling the transcriptional activity of the Xenopus tRNA and one particular 5S gene. When the input topology is supercoiled, high levels of transcription are observed, whereas input relaxed DNA is transcribed to a much lower extent. Transcription from an input relaxed template is stimulated by the addition of supercoiled nonspecific, vector DNA. Furthermore, in direct competition experiments, supercoiled DNA molecules were shown to be transcriptionally dominant over relaxed DNA molecules. Taken together, these data suggest that the efficiency with which a repressor or activator binding protein interacts with DNA may be significantly influenced by the topological status of its target. We demonstrate that modulation of reaction parameters which alter the normal topological processing events catalyzed by the S-150 can dramatically influence the level of gene expression.
Attardi,
Purification and characterization of Xenopus laevis type I topoisomerase.
1981, Pubmed,
Xenbase
Attardi,
Purification and characterization of Xenopus laevis type I topoisomerase.
1981,
Pubmed
,
Xenbase Di Mauro,
Activation of in vitro transcription and topology of closed DNA domains.
1985,
Pubmed Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Garg,
Role of DNA topoisomerase I in the transcription of supercoiled rRNA gene.
1987,
Pubmed Gargiulo,
Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
1984,
Pubmed
,
Xenbase Germond,
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
1975,
Pubmed Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase Hirose,
Enhanced transcription of fibroin gene in vitro on covalently closed circular templates.
1985,
Pubmed Kmiec,
The role of DNA-mediated transfer of TFIIIA in the concerted gyration and differential activation of the Xenopus 5S RNA genes.
1986,
Pubmed
,
Xenbase Kmiec,
The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts.
1985,
Pubmed
,
Xenbase Kmiec,
Gyration is required for 5S RNA transcription from a chromatin template.
1986,
Pubmed
,
Xenbase Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase Matsui,
Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly.
1987,
Pubmed Paulson,
The structure of histone-depleted metaphase chromosomes.
1977,
Pubmed Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase Pruitt,
Effect of topological constraint on transcription of ribosomal DNA in Xenopus oocytes. Comparison of plasmid and endogenous genes.
1984,
Pubmed
,
Xenbase Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Shure,
The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.
1977,
Pubmed Stirdivant,
DNA supercoiling affects in vitro transcription of two maize chloroplast genes differently.
1985,
Pubmed Wang,
DNA topoisomerases.
1985,
Pubmed Weintraub,
Expression of transfected DNA depends on DNA topology.
1986,
Pubmed Wilson,
A large region controls tRNA gene transcription.
1985,
Pubmed Worcel,
On the structure of the folded chromosome of Escherichia coli.
1972,
Pubmed Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
