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Mutational analysis of the 5' non-coding region of human immunodeficiency virus type 1: effects of secondary structure on translation.
Parkin NT, Cohen EA, Darveau A, Rosen C, Haseltine W, Sonenberg N.
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The first 111 nt from the 5' end of human immunodeficiency virus type 1 (HIV-1) mRNAs are shown to have a strong inhibitory effect on the translation of mRNA in in vitro translation extracts as well as in Xenopus oocytes. Mutations in the sequence of the 5' untranslated region (UTR) designed to disrupt predicted secondary structure of this region relieve the inhibition. Inhibition is restored by mutations that reconstruct the predicted secondary structure. The accessibility of the 5'-terminal cap structure was also found to be increased by some of these mutations. We conclude that secondary structure in the 5' UTR of HIV-1 mRNAs and resultant inaccessibility of the cap structure is responsible for the inhibition of translation. The implications of these findings for the understanding of the life cycle of HIV-1 are discussed.
Arya,
Trans-activator gene of human T-lymphotropic virus type III (HTLV-III).
1985, Pubmed
Arya,
Trans-activator gene of human T-lymphotropic virus type III (HTLV-III).
1985,
Pubmed Barré-Sinoussi,
Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).
1983,
Pubmed Cullen,
Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism.
1986,
Pubmed Curran,
The epidemiology of AIDS: current status and future prospects.
1985,
Pubmed Feinberg,
HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.
1986,
Pubmed Gallo,
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
1984,
Pubmed Godefroy-Colburn,
Cap accessibility correlates with the initiation efficiency of alfalfa mosaic virus RNAs.
1985,
Pubmed Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed Kao,
Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
,
Pubmed Knight,
Expression of the art/trs protein of HIV and study of its role in viral envelope synthesis.
1987,
Pubmed Losson,
Interference of nonsense mutations with eukaryotic messenger RNA stability.
1979,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Muesing,
Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein.
1987,
Pubmed Okamoto,
Demonstration of virus-specific transcriptional activator(s) in cells infected with HTLV-III by an in vitro cell-free system.
1986,
Pubmed Patarca,
Transcription directed by the HIV long terminal repeat in vitro.
1987,
Pubmed Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed Pelletier,
The involvement of mRNA secondary structure in protein synthesis.
1987,
Pubmed Pelletier,
Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
1985,
Pubmed Pelletier,
Photochemical cross-linking of cap binding proteins to eucaryotic mRNAs: effect of mRNA 5' secondary structure.
1985,
Pubmed Peterlin,
Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus.
1986,
Pubmed Popovic,
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
1984,
Pubmed Rose,
Inhibition of translation by poliovirus: inactivation of a specific initiation factor.
1978,
Pubmed Rosen,
Intragenic cis-acting art gene-responsive sequences of the human immunodeficiency virus.
1988,
Pubmed Rosen,
Post-transcriptional regulation accounts for the trans-activation of the human T-lymphotropic virus type III.
,
Pubmed Rosen,
The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
1985,
Pubmed Sadaie,
Site-directed mutagenesis of two trans-regulatory genes (tat-III,trs) of HIV-1.
1988,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sodroski,
Trans-acting transcriptional regulation of human T-cell leukemia virus type III long terminal repeat.
1985,
Pubmed Sodroski,
A second post-transcriptional trans-activator gene required for HTLV-III replication.
,
Pubmed Sodroski,
Location of the trans-activating region on the genome of human T-cell lymphotropic virus type III.
1985,
Pubmed Tinoco,
Improved estimation of secondary structure in ribonucleic acids.
1973,
Pubmed Wright,
Expression and characterization of the trans-activator of HTLV-III/LAV virus.
1986,
Pubmed Zagury,
Long-term cultures of HTLV-III--infected T cells: a model of cytopathology of T-cell depletion in AIDS.
1986,
Pubmed Zhang,
Double stranded DNA sequencing as a choice for DNA sequencing.
1988,
Pubmed Zitomer,
Transcriptional regulation of the yeast cytochrome c gene.
1979,
Pubmed Zoller,
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.
1982,
Pubmed
