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Transcriptional signals of a U4 small nuclear RNA gene.
McNamara KJ, Walker RJ, Roebuck KA, Stumph WE.
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The signals controlling the expression of a chicken U4 small nuclear RNA (snRNA) gene have been studied by microinjection into Xenopus oocytes. At least two distinct regions in the 5'-flanking DNA contribute to U4B RNA gene expression. The proximal regulatory element, which is inactivated by a 5'-flanking DNA deletion to position -38, provides a basal level of U4B RNA synthesis. The distal regulatory region, centered near position -200, acts as a transcriptional enhancer. It provides a 4-5 fold stimulation of U4B RNA gene expression above the basal level, and, like mRNA enhancers, is composed of multiple functional motifs. One of these, the octamer sequence ATGCAAAG, has previously been recognized as an important element of U1 and U2 snRNA gene enhancers, as well as being involved in the expression of a number of mRNA genes. However, the octamer sequence is not sufficient for U4B enhancer activity. An additional element, an "Sph motif," is located 12 base pairs downstream of the octamer and is an essential component of the U4B enhancer. Transcriptional competition studies indicate that the U4B and U1 snRNA genes utilize a common set of transcription factors.
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3684593 ???displayArticle.pmcLink???PMC306465 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985, Pubmed,
Xenbase
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985,
Pubmed
,
Xenbase Bark,
Genes for human U4 small nuclear RNA.
1986,
Pubmed
,
Xenbase Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986,
Pubmed Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed Black,
Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.
1986,
Pubmed Bohmann,
A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes.
,
Pubmed
,
Xenbase Chabot,
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
1985,
Pubmed Ciliberto,
Transcription signals in embryonic Xenopus laevis U1 RNA genes.
1985,
Pubmed
,
Xenbase Ciliberto,
Properties of a U1 RNA enhancer-like sequence.
1987,
Pubmed
,
Xenbase Davidson,
Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts.
,
Pubmed Deyerle,
Analysis of the early regulatory region of the human papovavirus BK.
1987,
Pubmed Earley,
Three linked chicken U1 RNA genes have limited flanking DNA sequence homologies that reveal potential regulatory signals.
1984,
Pubmed Eliceiri,
Formation of low molecular weight RNA species in HeLa cells.
1980,
Pubmed Hoffman,
Structural and functional analysis of chicken U4 small nuclear RNA genes.
1986,
Pubmed
,
Xenbase Howard,
Functional, developmentally expressed genes for mouse U1a and U1b snRNAs contain both conserved and non-conserved transcription signals.
1986,
Pubmed
,
Xenbase Korf,
Chicken U2 and U1 RNA genes are found in very different genomic environments but have similar promoter structures.
1986,
Pubmed Krainer,
Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
1985,
Pubmed Krol,
The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
1985,
Pubmed
,
Xenbase Mangin,
Human U2 small nuclear RNA genes contain an upstream enhancer.
1986,
Pubmed
,
Xenbase Marzluff,
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
1983,
Pubmed Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase Murphy,
Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition.
1987,
Pubmed
,
Xenbase Murphy,
Transcription of a gene for human U1 small nuclear RNA.
1982,
Pubmed
,
Xenbase Ondek,
Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities.
1987,
Pubmed Parslow,
Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
1984,
Pubmed Roop,
Structure and expression of a chicken gene coding for U1 RNA.
1981,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schirm,
The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity.
1987,
Pubmed Singh,
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
,
Pubmed Sive,
Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
1986,
Pubmed Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
1984,
Pubmed
,
Xenbase Staudt,
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.
,
Pubmed Stroke,
Genes and pseudogenes for rat U3A and U3B small nuclear RNA.
1985,
Pubmed
,
Xenbase Westin,
Human U2 and U1 RNA genes use similar transcription signals.
1984,
Pubmed
,
Xenbase Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
1984,
Pubmed
,
Xenbase Zenke,
Multiple sequence motifs are involved in SV40 enhancer function.
1986,
Pubmed Zhuang,
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.
1986,
Pubmed
