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The highly conserved U small nuclear RNA 3'-end formation signal is quite tolerant to mutation.
Ach RA, Weiner AM.
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Formation of the 3' end of U1 and U2 small nuclear RNA (snRNA) precursors is directed by a conserved sequence called the 3' box located 9 to 28 nucleotides downstream of all metazoan U1 to U4 snRNA genes sequenced so far. Deletion of part or all of the 3' box from human U1 and U2 genes drastically reduces 3'-end formation. To define the essential nucleotides within this box that direct 3'-end formation, we constructed a set of point mutations in the conserved residues of the human U1 3' box. The ability of the various mutations to direct 3'-end formation was tested by microinjection into Xenopus oocytes and transfection into HeLa cells. We found that the point mutations had diverse effects on 3'-end formation, ranging from no effect at all to severe inhibition; however, no single or double point mutation we tested completely eliminated 3'-end formation. We also showed that a rat U3 3' flank can effectively substitute for the human U1 3' flank, indicating that the 3' boxes of the different U snRNA genes are functionally equivalent.
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985, Pubmed,
Xenbase
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985,
Pubmed
,
Xenbase Birnstiel,
Transcription termination and 3' processing: the end is in site!
1985,
Pubmed Chandrasekharappa,
Biosynthesis of small nuclear RNAs in human cells.
1983,
Pubmed Ciliberto,
Formation of the 3' end on U snRNAs requires at least three sequence elements.
1986,
Pubmed
,
Xenbase Ciliberto,
Transcription signals in embryonic Xenopus laevis U1 RNA genes.
1985,
Pubmed
,
Xenbase de Vegvar,
3' end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters.
1986,
Pubmed Earley,
Three linked chicken U1 RNA genes have limited flanking DNA sequence homologies that reveal potential regulatory signals.
1984,
Pubmed Eliceiri,
Small RNAs in the nucleus and cytoplasm of HeLa cells.
1976,
Pubmed Eliceiri,
Formation of low molecular weight RNA species in HeLa cells.
1980,
Pubmed Forbes,
Differential expression of multiple U1 small nuclear RNAs in oocytes and embryos of Xenopus laevis.
1984,
Pubmed
,
Xenbase Frederiksen,
The differential inhibitory effect of alpha-amanitin on the synthesis of low molecular weight RNA components in BHK cells.
1978,
Pubmed Georgiev,
The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processing.
1985,
Pubmed Gick,
Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
1986,
Pubmed Gil,
A sequence downstream of AAUAAA is required for rabbit beta-globin mRNA 3'-end formation.
,
Pubmed Graves,
Delineation of transcriptional control signals within the Moloney murine sarcoma virus long terminal repeat.
1985,
Pubmed
,
Xenbase Hart,
Definition of essential sequences and functional equivalence of elements downstream of the adenovirus E2A and the early simian virus 40 polyadenylation sites.
1985,
Pubmed Hashimoto,
A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro.
1986,
Pubmed Hentschel,
Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase Hernandez,
Formation of the 3' end of U1 snRNA requires compatible snRNA promoter elements.
1986,
Pubmed Hernandez,
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
1985,
Pubmed Hoffman,
Structural and functional analysis of chicken U4 small nuclear RNA genes.
1986,
Pubmed
,
Xenbase Joyce,
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
1983,
Pubmed Korf,
Chicken U2 and U1 RNA genes are found in very different genomic environments but have similar promoter structures.
1986,
Pubmed Krol,
The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
1985,
Pubmed
,
Xenbase Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed Kunkel,
Transcription boundaries of U1 small nuclear RNA.
1985,
Pubmed Lund,
The two embryonic U1 small nuclear RNAs of Xenopus laevis are encoded by a major family of tandemly repeated genes.
1984,
Pubmed
,
Xenbase Madore,
Intracellular site of U1 small nuclear RNA processing and ribonucleoprotein assembly.
1984,
Pubmed Mangin,
Human U2 small nuclear RNA genes contain an upstream enhancer.
1986,
Pubmed
,
Xenbase Manser,
Human U1 loci: genes for human U1 RNA have dramatically similar genomic environments.
1982,
Pubmed Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase Matteucci,
Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon.
1983,
Pubmed McLauchlan,
The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini.
1985,
Pubmed Moore,
Site-specific polyadenylation in a cell-free reaction.
1984,
Pubmed Moore,
Accurate cleavage and polyadenylation of exogenous RNA substrate.
1985,
Pubmed Murphy,
Transcription of a gene for human U1 small nuclear RNA.
1982,
Pubmed
,
Xenbase Saba,
Isolation and characterization of two putative full-length Drosophila U4 small nuclear RNA genes.
1986,
Pubmed
,
Xenbase Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
1984,
Pubmed
,
Xenbase Stroke,
Genes and pseudogenes for rat U3A and U3B small nuclear RNA.
1985,
Pubmed
,
Xenbase Van Arsdell,
Human genes for U2 small nuclear RNA are tandemly repeated.
1984,
Pubmed Westin,
Human U2 and U1 RNA genes use similar transcription signals.
1984,
Pubmed
,
Xenbase Yuo,
Sequences required for 3' end formation of human U2 small nuclear RNA.
1985,
Pubmed
,
Xenbase Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
1984,
Pubmed
,
Xenbase Zoller,
Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.
1984,
Pubmed
