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In Vitro Cell Dev Biol
1987 May 01;235:323-31. doi: 10.1007/bf02620988.
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The effects of various nutritional supplements on the growth, migration and differentiation of Xenopus laevis neural crest cells in vitro.
Wilson HC, Milos NC.
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This study investigates the nutritional requirements of Xenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required to enhance culture development. These include insulin, alpha-melanocyte stimulating hormone, somatotropin, luteotrophic hormone, linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conducive environment tested for cell migration and adhesion.
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