Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1987 Feb 01;62:477-83. doi: 10.1002/j.1460-2075.1987.tb04778.x.
Show Gene links
Show Anatomy links
Enzymatic conversion of guanosine 3' adjacent to the anticodon of yeast tRNAPhe to N1-methylguanosine and the wye nucleoside: dependence on the anticodon sequence.
Droogmans L, Grosjean H.
???displayArticle.abstract???
N1-Methylguanosine (m1G) or wye nucleoside (Y) are found 3' adjacent to the anticodon (position 37) of eukaryotic tRNAPhe. The biosynthesis of these two modified nucleosides has been investigated. The importance of the type of nucleosides in the anticodon of yeast tRNAPhe on the potentiality of this tRNA to be a substrate for the corresponding maturation enzyme has also been studied. This involved microinjection into Xenopus laevis oocytes and incubation in a yeast extract of restructured yeast tRNAPhe in which the anticodon GmAA and the 3' adjacent Y nucleoside were substituted by various tetranucleotides ending with a guanosine. The results obtained by oocyte microinjection indicate: that all the restructured yeast tRNAsPhe are efficient substrates for the tRNA (guanosine-37 N1)methyltransferase. This means that the anticodon sequence is not critical for the tRNA recognition by this enzyme; in contrast, for Y nucleoside biosynthesis, the anticodon sequence GAA is an absolute requirement; the conversion of G-37 into Y-37 nucleoside is a multienzymatic process in which m1G-37 is the first obligatory intermediate; all the corresponding enzymes are cytoplasmic. In a crude yeast extract, restructured yeast tRNAPhe with G-37 is efficiently modified only into m1G-37; the corresponding enzyme is a S-adenosyl-L-methionine-dependent tRNA methyltransferase. The pure Escherichia coli tRNA (guanosine-37 N1) methyltransferase is unable to modify the guanosine-37 of yeast tRNAPhe.
Blobstein,
Structure of the fluorescent nucleoside of yeast phenylalanine transfer ribonucleic acid.
1975, Pubmed
Blobstein,
Structure of the fluorescent nucleoside of yeast phenylalanine transfer ribonucleic acid.
1975,
Pubmed Bruce,
Enzymatic replacement of the anticodon of yeast phenylalanine transfer ribonucleic acid.
1982,
Pubmed Bruce,
Replacement of anticodon loop nucleotides to produce functional tRNAs: amber suppressors derived from yeast tRNAPhe.
1982,
Pubmed Carbon,
Site-directed in vitro replacement of nucleosides in the anticodon loop of tRNA: application to the study of structural requirements for queuine insertase activity.
1983,
Pubmed
,
Xenbase Carbon,
Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes.
1982,
Pubmed De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase Droogmans,
Enzymatic 2'-O-methylation of the wobble nucleoside of eukaryotic tRNAPhe: specificity depends on structural elements outside the anticodon loop.
1986,
Pubmed
,
Xenbase Feinberg,
Isolation and characterization of peroxy-Y base from phenylalanine transfer ribonucleic acid of the plant, Lupinus luteus.
1974,
Pubmed Feldmann,
Transfer ribonucleic acid from Mycoplasma laidlawii A.
1971,
Pubmed Fournier,
Post-transcriptional modification of the wobble nucleotide in anticodon-substituted yeast tRNAArgII after microinjection into Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase Gatica,
Aminoacylation of transfer RNA microinjected into Xenopus laevis oocytes.
1975,
Pubmed
,
Xenbase Gillam,
The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.
1967,
Pubmed Haumont,
Enzymatic conversion of adenosine to inosine in the wobble position of yeast tRNAAsp: the dependence on the anticodon sequence.
1984,
Pubmed
,
Xenbase Hjalmarsson,
Purification and characterization of transfer RNA (guanine-1)methyltransferase from Escherichia coli.
1983,
Pubmed Kasai,
Isolation of hydroxy-Y base from rat liver tRNAPhe.
1979,
Pubmed Kasai,
Structure of wye (Yt base) and wyosine (Yt) from Torulopsis utilis phenylalanine transfer ribonucleic acid.
1976,
Pubmed Kuchino,
Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.
1982,
Pubmed Li,
Biosynthetic studies of the Y base in yeast phenylalanine tRNA. Incorporation of guanine.
1973,
Pubmed Maelicke,
The structure of the anticodon loop of tRNAPhe from yeast as deduced from spectroscopic studies on oligonucleotides.
1975,
Pubmed Mazabraud,
The nucleotide sequence of phenylalanine tRNA of Xenopus laevis.
1982,
Pubmed
,
Xenbase Münch,
Biosynthesis of the nucleoside Y in yeast tRNAPhe: incorporation of the 3-amino-3-carboxypropyl-group from methionine.
1975,
Pubmed Nakanishi,
Structure of the "peroxy-Y base" from liver tRNA Phe .
1971,
Pubmed Pergolizzi,
Incorporation of lysine into Y base of phenylalanine tRNA in Vero cells.
1979,
Pubmed Silberklang,
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
1979,
Pubmed Sindhuphak,
Site specificities of three transfer RNA methyltransferases from yeast.
1985,
Pubmed Smith,
Nuclear magnetic resonance signal assignments of purified [13C]methyl-enriched yeast phenylalanine transfer ribonucleic acid.
1985,
Pubmed Smolar,
Two transfer RNA (1-methylguanine) methylases from yeast.
1975,
Pubmed Sprinzl,
Compilation of tRNA sequences.
1985,
Pubmed Thiebe,
A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid.
1968,
Pubmed
