Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The use of mRNA translation in vitro and in ovo followed by crossed immunoelectrophoretic autoradiography to study the biosynthesis of human cholinesterases.
Soreq H, Dziegielewska KM, Zevin-Sonkin D, Zakut H.
???displayArticle.abstract???
The synthesis of various cholinesterases in different fetal human tissues was studied using in vitro and in ovo translation of poly(A)+ RNA, followed by crossed immunoelectrophoretic autoradiography. When unfractionated poly(A)+ mRNA from fetal brain, muscle, or liver was translated in vitro, in the reticulocyte lysate cell-free system, polypeptides were synthesized which reacted with antibodies against either "true" acetylcholinesterase (acetylcholine hydrolase; EC 3.1.1.7) or "pseudo", butyrylcholinesterase (acylcholine acylhydrolase; EC 3.1.1.8). The two nascent cholinesterases could be separated by crossed immunoelectrophoresis followed by autoradiography, suggesting that acetylcholinesterase and butyrylcholinesterase are produced in all three tissues from nascent polypeptides containing different immunological domains. To examine whether the biosynthesis of cholinesterases includes posttranslational processing events, Xenopus oocytes were microinjected with mRNA from these tissues. Immunoelectrophoretic analysis of oocyte intracellular homogenates and incubation medium revealed various precipitation arcs, reflecting the synthesis and posttranslational processing of multiple forms of tissue-specific exported and intracellular acetylcholinesterase and butyrylcholinesterase. These findings demonstrate that polymorphic cholinesterases are produced from nascent polypeptide products which undergo further posttranslational processing events in a tissue-specific manner before they become mature compartmentalized cholinesterases.
AUSTIN,
Two selective inhibitors of cholinesterase.
1953, Pubmed
AUSTIN,
Two selective inhibitors of cholinesterase.
1953,
Pubmed Axelsen,
Intermediate gel in crossed and in fused rocket immunoelectrophoresis.
1973,
Pubmed Brimijoin,
Production and characterization of separate monoclonal antibodies to human acetylcholinesterase and butyrylcholinesterase.
1983,
Pubmed Burmeister,
Production of EGF-containing polypeptides in Xenopus oocytes microinjected with submaxillary gland mRNA.
1984,
Pubmed
,
Xenbase Chautard-Freire-Maia,
Probable assignment of the serum cholinesterase (E1) and transferrin (Tf) loci to chromosome 1 in man.
1977,
Pubmed Coates,
Genetic variation in human erythrocyte acetylcholinesterase.
1972,
Pubmed Dutta-Choudhury,
Human erythrocyte acetylcholinesterase is an amphipathic protein whose short membrane-binding domain is removed by papain digestion.
1984,
Pubmed Dziegielewska,
Messenger ribonucleic acid (mRNA) from developing rat cerebellum directs in vitro synthesis of plasma proteins.
1985,
Pubmed Dziegielewska,
Synthesis of plasma proteins in fetal, adult, and neoplastic human brain tissue.
1986,
Pubmed
,
Xenbase Dziegielewska,
Synthesis of plasma proteins by rat fetal brain and choroid plexus.
1984,
Pubmed Eliyahu,
Degranulation of rat cerebellum induces selective variations in gene expression.
1982,
Pubmed Fambrough,
Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies revealed by monoclonal antibodies.
1982,
Pubmed Futerman,
A hydrophobic dimer of acetylcholinesterase from Torpedo californica electric organ is solubilized by phosphatidylinositol-specific phospholipase C.
1983,
Pubmed Futerman,
Solubilization of membrane-bound acetylcholinesterase by a phosphatidylinositol-specific phospholipase C.
1985,
Pubmed Herman,
Muscle-specific expression of a gene affecting acetylcholinesterase in the nematode caenorhabditis elegans.
1985,
Pubmed Johnson,
An acetylcholinesterase-deficient mutant of the nematode Caenorhabditis elegans.
1981,
Pubmed KARNOVSKY,
A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.
1964,
Pubmed LAURELL,
ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS.
1965,
Pubmed Lovrien,
Serum cholinesterase (E2) linkage analysis: possible evidence for localization to chromosome 16.
1978,
Pubmed Marsh,
An immunological study of rat acetylcholinesterase: comparison with acetylcholinesterases from other vertebrates.
1984,
Pubmed Massoulié,
The molecular forms of cholinesterase and acetylcholinesterase in vertebrates.
1982,
Pubmed Ott,
Membrane acetylcholinesterases: purification, molecular properties and interactions with amphiphilic environments.
1985,
Pubmed Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed Prody,
Use of synthetic oligodeoxynucleotide probes for the isolation of a human cholinesterase cDNA clone.
1986,
Pubmed Rakonczay,
Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species.
1986,
Pubmed Rosenfeld,
Altered expression of the calcitonin gene associated with RNA polymorphism.
1981,
Pubmed Rotundo,
Asymmetric acetylcholinesterase is assembled in the Golgi apparatus.
1984,
Pubmed Schumacher,
Primary structure of Torpedo californica acetylcholinesterase deduced from its cDNA sequence.
,
Pubmed Sikorav,
Synthesis in vitro of precursors of the catalytic subunits of acetylcholinesterase from Torpedo marmorata and Electrophorus electricus.
1984,
Pubmed Soreq,
A human acetylcholinesterase gene identified by homology to the Ace region of Drosophila.
1985,
Pubmed
,
Xenbase Soreq,
The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes.
1985,
Pubmed
,
Xenbase Soreq,
Expression of cholinesterase gene(s) in human brain tissues: translational evidence for multiple mRNA species.
1984,
Pubmed
,
Xenbase Sparkes,
Genetic linkage studies of transferrin, pseudocholinesterase, and chromosome 1 loci.
1984,
Pubmed Vigny,
"Nonspecific" cholinesterase and acetylcholinesterase in rat tissues: molecular forms, structural and catalytic properties, and significance of the two enzyme systems.
1978,
Pubmed Weitz,
Characterization of an active hydrophilic erythrocyte membrane acetylcholinesterase obtained by limited proteolysis of the purified enzyme.
1984,
Pubmed Zakut,
Polymorphism of acetylcholinesterase in discrete regions of the developing human fetal brain.
1985,
Pubmed
,
Xenbase Zevin-Sonkin,
Expression of acetylcholinesterase gene(s) in the human brain: molecular cloning evidence for cross-homologous sequences.
1985,
Pubmed
,
Xenbase
