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Proc Natl Acad Sci U S A
1986 Mar 01;836:1598-1602.
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Trans effect of the E1 region of adenoviruses on the expression of a prokaryotic gene in mammalian cells: resistance to 5' -CCGG- 3' methylation.
Langner KD, Weyer U, Doerfler W.
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The plasmid construct pSVO-CAT has been used to test adenovirus promoter activities in the unmethylated or methylated state. We have now observed that the E2A late promoter of adenovirus type 2 (Ad2) DNA also activated the chloramphenicol acetyltransferase (CAT) gene upon transfection of the pAd2E2A-CAT construct into mammalian cells, and it was inactivated by specific methylations of three 5' -CCGG- 3' sites. Similar results had been reported previously after microinjecting promoter-methylated constructs into oocytes of Xenopus laevis. Surprisingly, it was found that the pSVO-CAT construct, which lacked eukaryotic promoter sequences, was able to express the CAT gene upon transfection into human or hamster cells that harbored and constitutively expressed the E1 region of Ad2 or Ad5 DNA. In these cells, the expression of the pAd2E2A-CAT construct was enhanced, but it was only partly sensitive to DNA methylation, possibly because DNA methylation was counteracted directly or indirectly by E1 functions. The pSVO-CAT construct was also expressed in HeLa or BHK21 cells upon cotransfection with a plasmid carrying the HindIII-G fragment of Ad2 DNA that contained the E1A region and part of the E1B region. By mapping pSVO-CAT-specific RNAs, we could demonstrate that pSVO-CAT activity in Ad2- or Ad5-transformed cells was mediated by prokaryotic promoter-like sequences in the pBR322 section of the construct, and it presumably functioned via trans-activation mediated by the E1 region. This trans-activation of pSVO-CAT in adenovirus-transformed cells was partly insensitive to DNA methylation.
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Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
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Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.
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Host response to adenovirus 2-transformed hamster embryo cells.
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Intracellular forms of Adenovirus DNA. II. Isolation in dye-buoyant density gradients of a DNA-RNA complex from KB cells infected with Adenovirus type 2.
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Viral gene products in adenovirus type-2 transformed hamster cells.
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Low molecular weight RNAs with homologies to cellular DNA at sites of adenovirus DNA insertion in hamster or mouse cells.
1984,
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Patch homologies and the integration of adenovirus DNA in mammalian cells.
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Integration of viral DNA into the genome of the adenovirus type 2-transformed hamster cell line HE5 without loss or alteration of cellular nucleotides.
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
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Transformation of rat cells by DNA of human adenovirus 5.
1973,
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Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
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Transcriptional activation of cloned human beta-globin genes by viral immediate-early gene products.
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An adenovirus type 5 early gene function regulates expression of other early viral genes.
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Adenovirus types 2 and 5 functions elicit replication and late expression of adenovirus type 12 DNA in hamster cells.
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The promoter of the late p10 gene in the insect nuclear polyhedrosis virus Autographa californica: activation by viral gene products and sensitivity to DNA methylation.
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Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression.
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1984,
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,
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Transcription of overlapping sets of RNAs from the genome of Autographa californica nuclear polyhedrosis virus: a novel method for mapping RNAs.
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Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
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Expression of viral DNA in adenovirus type 12-transformed cells, in tumor cells, and in revertants.
1981,
Pubmed Scott,
Activation of mouse genes in transformed cells.
1983,
Pubmed Smith,
Physical Analysis of Autographa californica Nuclear Polyhedrosis Virus Transcripts for Polyhedrin and 10,000-Molecular-Weight Protein.
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Pubmed Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed Stabel,
Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells.
1980,
Pubmed Strohl,
The response of BHK21 cells to infection with type 12 adenovirus. 3. Transformation and restricted replication of superinfecting type 2 adenovirus.
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Pubmed Stüber,
Organization of transcriptional signals in plasmids pBR322 and pACYC184.
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Pubmed Sutter,
Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells.
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,
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Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
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Pubmed
