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Stimulation of sea urchin H2B histone gene transcription by a chromatin-associated protein fraction depends on gene sequences downstream of the transcription start site.
Mous J, Stunnenberg H, Georgiev O, Birnstiel ML.
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We isolated a chromosomal protein fraction derived from chromatin of sea urchin embryos which specifically stimulated the expression of the histone H2B gene by a factor of 5- to 10-fold when the complete sea urchin histone gene repeat h22 was injected in Xenopus laevis oocyte nuclei. Gene manipulation experiments revealed the existence of two different target sites in the H2B gene which appear to mediate the response to injection of the stimulatory sea urchin chromatin-associated proteins; both are located downstream of the transcription initiation site. The first sequence element which is shown to be implicated is within, or at least includes, the H2B 5' untranslated leader sequence between nucleotides 11 and 76. The second element resides within an H2B DNA segment located near the 3' end of the gene, extending from 90 base pairs upstream of the mRNA 3' terminus to 140 base pairs in the spacer sequences downstream.
Banerji,
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
1983, Pubmed
Banerji,
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
1983,
Pubmed Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983,
Pubmed
,
Xenbase Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase Clerc,
Transcription of a cloned Xenopus laevis H4 histone gene in the homologous frog oocyte system depends on an evolutionary conserved sequence motif in the -50 region.
1983,
Pubmed
,
Xenbase Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Xenbase Georgiev,
The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processing.
1985,
Pubmed Georgiev,
Processing and nucleo-cytoplasmic transport of histone gene transcripts.
1984,
Pubmed
,
Xenbase Gillies,
A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
1983,
Pubmed Gross,
Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.
1976,
Pubmed Grosschedl,
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
1980,
Pubmed
,
Xenbase Grosschedl,
Delimitation of far upstream sequences required for maximal in vitro transcription of an H2A histone gene.
1982,
Pubmed Hentschel,
Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase Kedes,
Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
1971,
Pubmed Maxam,
A new method for sequencing DNA.
1977,
Pubmed Messing,
New M13 vectors for cloning.
1983,
Pubmed Moore,
The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.
1985,
Pubmed Newrock,
Stage-specific mRNAs coding for subtypes of H2A and H2B histones in the sea urchin embryo.
1978,
Pubmed Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase Spelsberg,
Deoxyribonucleoproteins and the tissue-specific restriction of the deoxyribonucleic acid in chromatin.
1970,
Pubmed Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Xenbase Stunnenberg,
Bioassay for components regulating eukaryotic gene expression: a chromosomal factor involved in the generation of histone mRNA 3' termini.
1982,
Pubmed
,
Xenbase Wright,
DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells.
1984,
Pubmed
