Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.
Filipowicz W, Konarska M, Gross HJ, Shatkin AJ.
???displayArticle.abstract???
HeLa cell extract contains RNA ligase activity that converts linear polyribonucleotides to covalently closed circles. RNA substrates containing 2',3'-cyclic phosphate and 5'-hydroxyl termini are circularized by formation of a normal 3',5' phosphodiester bond. This activity differs from a previously described wheat germ RNA ligase which circularizes molecules with 2',3'-cyclic and 5' phosphate ends by a 2'-phosphomonester, 3',5'-phosphodiester linkage (Konarska et al., Nature 293, 112-116, 1981; Proc. Natl. Acad. Sci. USA 79, 1474-1478, 1982). The HeLa cell ligase can also utilize molecules with 3'-phosphate ends. However, in this case ligation is preceded by an ATP-dependent conversion of the 3'-terminal phosphate to the 2',3' cyclic form by a novel activity, RNA 3'-terminal phosphate cyclase. Both RNA ligase and RNA 3'-terminal phosphate cyclase activities are also present in extract of Xenopus oocyte nuclei, consistent with a role in RNA processing.
Abelson,
RNA processing and the intervening sequence problem.
1979, Pubmed
Abelson,
RNA processing and the intervening sequence problem.
1979,
Pubmed Arnberg,
Some yeast mitochondrial RNAs are circular.
1980,
Pubmed Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase Branch,
Cell-free circularization of viroid progeny RNA by an RNA ligase from wheat germ.
1982,
Pubmed Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed Cech,
In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.
1981,
Pubmed De Robertis,
Intranuclear location of the tRNA splicing enzymes.
1981,
Pubmed
,
Xenbase England,
Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.
1978,
Pubmed Filipowicz,
Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
1983,
Pubmed
,
Xenbase Grabowski,
The intervening sequence of the ribosomal RNA precursor is converted to a circular RNA in isolated nuclei of Tetrahymena.
1981,
Pubmed Gross,
Nucleotide sequence and secondary structure of potato spindle tuber viroid.
1978,
Pubmed Halbreich,
A pathway of cytochrome b mRNA processing in yeast mitochondria: specific splicing steps and an intron-derived circular DNA.
1980,
Pubmed Haseloff,
Comparative sequence and structure of viroid-like RNAs of two plant viruses.
1982,
Pubmed Hinton,
The preparative synthesis of oligodeoxyribonucleotides using RNA ligase.
1982,
Pubmed Kaufmann,
Covalent joining of phenylalanine transfer ribonucleic acid half-molecules by T4 RNA ligase.
1974,
Pubmed Kikuchi,
Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.
1982,
Pubmed Konarska,
Binding of ribosomes to linear and circular forms of the 5'-terminal leader fragment of tobacco-mosaic-virus RNA.
1981,
Pubmed Konarska,
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
,
Pubmed Konarska,
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.
1982,
Pubmed Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed Otsuka,
Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors.
1981,
Pubmed
,
Xenbase Peebles,
Splicing of yeast tRNA precursors: a two-stage reaction.
1979,
Pubmed Sogin,
2',3'-Cyclic NADP as a substrate for 2',3'-cyclic nucleotide 3'-phosphohydrolase.
1976,
Pubmed Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
