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DNA methylation patterns in the 5S DNAs of Xenopus laevis.
Sims MA, Doering JL, Hoyle HD.
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The frequency of cytosine methylation at specific sites in the somatic 5S DNA (X1s) and trace oocyte 5S DNA (X1t) of X. laevis has been determined using restriction enzymes that are inhibited by the presence of 5-methylcytosine (5mC) within their cleavage sequences. 5S DNA methylation patterns were determined in genomic DNA from mature red blood cells, which express neither type of 5S gene, and from liver, which expresses only X1s. All the sites examined in X1t are greater than 95% methylated in red cells and liver. In the X1s of red cells all the sites examined are methylated in greater than 95% of repeats, while in liver some sites are modified in only 90% of repeats. Repeats containing unmethylated sites are randomly distributed throughout the tandem arrays in both red cells and liver. The high levels of methylation for X1s are in marked contrast to the situation with other Xenopus genes which do have sites of significant undermethylation in tissues where they are active. Thus, undermethylation in active genetic regions may not be a general feature for all classes of eukaryotic genes.
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6298718 ???displayArticle.pmcLink???PMC325714 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Bird,
Variable patterns of total DNA and rDNA methylation in animals.
1980, Pubmed
Bird,
Variable patterns of total DNA and rDNA methylation in animals.
1980,
Pubmed Bird,
Loss of rDNA methylation accompanies the onset of ribosomal gene activity in early development of X. laevis.
1981,
Pubmed
,
Xenbase Brown,
Purification and some characteristics of 5S DNA from Xenopus laevis.
1971,
Pubmed
,
Xenbase Brown,
The isolation and characterization of a second oocyte 5s DNA from Xenopus laevis.
1977,
Pubmed
,
Xenbase Brown,
High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase Compere,
DNA methylation controls the inducibility of the mouse metallothionein-I gene lymphoid cells.
1981,
Pubmed Doering,
Identification and genomic organization of human tRNALys genes.
1982,
Pubmed
,
Xenbase DOSKOCIL,
Distribution of 5-methylcytosine in pyrimidine sequences of deoxyribonucleic acids.
1962,
Pubmed Ehrlich,
5-Methylcytosine in eukaryotic DNA.
1981,
Pubmed Ford,
Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
1973,
Pubmed
,
Xenbase Grippo,
Methylation of DNA in developing sea urchin embryos.
1968,
Pubmed Groudine,
Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation.
1981,
Pubmed Gurdon,
The transcription of 5 S DNA injected into Xenopus oocytes.
1978,
Pubmed
,
Xenbase Hoopes,
Studies on the selectivity of DNA precipitation by spermine.
1981,
Pubmed Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase Kuo,
DNA methylation: correlation with DNase I sensitivity of chicken ovalbumin and conalbumin chromatin.
1979,
Pubmed Macleod,
DNAase I sensitivity and methylation of active versus inactive rRNA genes in xenopus species hybrids.
1982,
Pubmed
,
Xenbase Mandel,
DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.
1979,
Pubmed McGhee,
Specific DNA methylation sites in the vicinity of the chicken beta-globin genes.
1979,
Pubmed McKeon,
Unusual methylation pattern of the alpha 2 (l) collagen gene.
1982,
Pubmed Miller,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
1978,
Pubmed
,
Xenbase Naveh-Many,
Active gene sequences are undermethylated.
1981,
Pubmed Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase Razin,
DNA methylation and gene function.
1980,
Pubmed Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed Shen,
Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.
1980,
Pubmed Sneider,
The 5'-cytosine in CCGG1 is methylated in two eukaryotic DNAs and Msp I is sensitive to methylation at this site.
1980,
Pubmed Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed Stein,
In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.
1982,
Pubmed Tata,
The expression of the vitellogenin gene.
1976,
Pubmed
,
Xenbase van der Ploeg,
DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.
1980,
Pubmed Vardimon,
Expression of a cloned adenovirus gene is inhibited by in vitro methylation.
1982,
Pubmed
,
Xenbase Wahli,
Cloning and characterization of synthetic sequences from the Xenopus iaevis vitellogenin structural gene.
1978,
Pubmed
,
Xenbase Wegnez,
Sequence heterogeneity of 5 S RNA in Xenopus laevis.
1972,
Pubmed
,
Xenbase Weintraub,
Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
1981,
Pubmed
