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Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
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A novel technique was developed for monitoring the level of the mRNA species that direct the synthesis of acetylcholinesterase (AcChoEase; acetylcholine acetylhydrolase, EC 3.1.1.7), using microinjected Xenopus oocytes as a translation system. When injected with poly(A)-containing RNA from whole rat brain or rat cerebellum and from electric organ of Torpedo ocellata, Xenopus oocytes synthesize and secrete catalytically active cholinesterase. The newly synthesized enzyme, which is mostly secreted into the oocytes incubation medium, appears to be primarily AcChoEase because it is inhibited by the specific inhibitor BW 284C51. The new enzymatic activity can be detected after injection of as little as 12.5 ng of poly(A)-containing RNA per oocyte, and there is a linear dependence of the oocytes' ability to form AcChoEase on the amount of injected RNA. The AcChoEase mRNA displays a tau 1/2 of about 10 +/- 3 hr in injected oocytes. The abundance of AcChoEase mRNA in the total nonfractionated mRNA injected was calculated to be ca. 1 x 10(-5), a value similar to the level of AcChoEase protein determined in rat brain. The combination of the high turnover number of AcChoEase, the efficiency of the oocyte system, and the sensitivity of the assay used thus permit the accurate monitoring of the scarce mRNA species that direct the synthesis of this enzyme.
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974, Pubmed,
Xenbase
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase AUSTIN,
Two selective inhibitors of cholinesterase.
1953,
Pubmed Bennett,
Acetylcholinesterase activity and weight measures in fifteen brain areas from six lines of rats.
1966,
Pubmed Betz,
Evolution of cholinergic proteins in developing slow and fast skeletal muscles in chick embryo.
1980,
Pubmed Blobel,
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
1975,
Pubmed Bon,
Collagen-tailed and hydrophobic components of acetylcholinesterase in Torpedo marmorata electric organ.
1980,
Pubmed Chan,
Translation of ovalbumin mRNA in Xenopus laevis oocytes. Characterization of the system and effects of estrogen on injected mRNA populations.
1976,
Pubmed
,
Xenbase Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Fernandez,
Neurotrophic control of 16S acetylcholinesterase at the vertebrate neuromuscular junction.
1979,
Pubmed Ghysdael,
Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.
1977,
Pubmed
,
Xenbase Gisiger,
A specific form of acetylcholinesterase is secreted by rat sympathetic ganglia.
1977,
Pubmed Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase Hall,
Multiple forms of acetylcholinesterase and their distribution in endplate and non-endplate regions of rat diaphragm muscle.
1973,
Pubmed Jedrzejczyk,
Molecular forms of the cholinesterases inside and outside muscle endplates.
1981,
Pubmed Johnson,
A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations.
1975,
Pubmed Kaplan,
Sequence complexity of polyadenylated RNA obtained from rat brain regions and cultured rat cells of neural origin.
1978,
Pubmed Karlin,
Chemical distinctions between acetylcholinesterase and the acetylcholine receptor.
1967,
Pubmed Kimhi,
Acetylcholinesterase in mouse neuroblastoma cells: intracellular and released enzyme.
1980,
Pubmed Koenig,
Neural induction of the 16S acetylcholinesterase in muscle cell cultures.
1978,
Pubmed Kusano,
Acetylcholine receptors in the oocyte membrane.
,
Pubmed
,
Xenbase Labarca,
MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
1977,
Pubmed
,
Xenbase Lai,
Brain regional distribution of glutamic acid decarboxylase, choline acetyltransferase, and acetylcholinesterase in the rat: effects of chronic manganese chloride administration after two years.
1981,
Pubmed Lanks,
Increased rate of acetylcholinesterase synthesis in differentiating neuroblastoma cells.
1974,
Pubmed Laskey,
Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
1977,
Pubmed
,
Xenbase Littauer,
The regulatory function of poly(A) and adjacent 3' sequences in translated RNA.
1982,
Pubmed Lømo,
Control of junctional acetylcholinesterase by neural and muscular influences in the rat.
1980,
Pubmed Meedel,
Development of acetylchilinesterase during embryogenesis of the ascidian Ciona intestinalis.
1979,
Pubmed Miskin,
Microinjected Xenopus oocytes synthesize active human plasminogen activator.
1981,
Pubmed
,
Xenbase Mohun,
The secretion of proteins in vitro from Xenopus oocytes and their accessory cells: a biochemical and morphological study.
1981,
Pubmed
,
Xenbase Nudel,
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
1976,
Pubmed
,
Xenbase Prives,
Appearance and disappearance of acetycholine receptor during differentiation of chick skeletal muscle in vitro.
1976,
Pubmed Rieger,
Molecular forms of acetylcholinesterase: their de novo synthesis in mouse neuroblastoma cells.
1976,
Pubmed Rotundo,
Synthesis, transport and fate of acetylcholinesterase in cultured chick embryos muscle cells.
1980,
Pubmed Rubin,
Regulation of acetylcholinesterase appearance at neuromuscular junctions in vitro.
1980,
Pubmed Shulman,
Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase.
1980,
Pubmed
,
Xenbase Silman,
Parallel regulation of acetylcholinesterase and pseudocholinesterase in normal, denervated and dystrophic chicken skeletal muscle.
1979,
Pubmed Soreq,
Secreted proteins in the medium of microinjected Xenopus oocytes are degraded by oocyte proteases.
1981,
Pubmed
,
Xenbase Soreq,
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
1974,
Pubmed Soreq,
Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells.
1980,
Pubmed Soreq,
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.
1981,
Pubmed
,
Xenbase Trevor,
Acetylcholinesterases.
1978,
Pubmed Valle,
Synthesis and secretion of mouse immunoglobulin chains from Xenopus oocytes.
1981,
Pubmed
,
Xenbase Vigny,
Active-site catalytic efficiency of acetylcholinesterase molecular forms in Electrophorus, torpedo, rat and chicken.
1978,
Pubmed Vijayan,
Polyacrylamide gel electrophoresis of rat brain acetylcholinesterase: isoenzymes of normal rat brain.
1974,
Pubmed Walker,
Regulation of acetylcholinesterase in chick muscle cultures after treatment with diisopropylphosphorofluoridate: ribonucleic acid and protein synthesis.
1976,
Pubmed Wilson,
Regulation of newly synthesized acetylcholinesterase in muscle cultures treated with diisopropylfluorophosphate.
1974,
Pubmed
