Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
2006 Oct 01;4531:1-9. doi: 10.1007/s00424-006-0102-5.
Show Gene links
Show Anatomy links
Route of glucocorticoid-induced macromolecules across the nuclear envelope as viewed by atomic force microscopy.
Shahin V.
???displayArticle.abstract???
Glucocorticoids are vital steroid hormones. The physiologic activities of these hydrophobic molecules predominantly require translocation of glucocorticoid-initiated macromolecules (GIMs), proteins and mRNA transcripts, in and out of the nucleus, respectively. The bidirectional transport of GIMs is mediated by nuclear pore complexes (NPCs) that span the nuclear envelope at regular distances. The transport proceeds through the NPC central channel, whose interior is lined up by hydrophobic proteins. The NPC channel is assumed to dilate while hydrophobic cargos are being translocated through. Upon glucocorticoid injection into a glucocorticoid-sensitive cell, Xenopus laevis oocyte, and using atomic force microscopy, we have recently unraveled the long unexplored paths that GIMs take through the nuclear envelope and described interactions of GIMs with NPCs. In so doing, surprising and intriguing observations were made and the following conclusions were drawn: glucocorticoid-initiated proteins evoke NPC channel dilation before physical interaction with the NPC. NPC channel dilation is apparently transmitted through binding of glucocorticoid-induced proteins to NPC-associated filaments or yet unknown structures in the cytoplasmic nuclear envelope surface. The transport of both proteins and ribonucleoproteins seems to be non-randomly confined to local areas on either nuclear envelope site, the so-called hot spots.
Bartholome,
Membrane glucocorticoid receptors (mGCR) are expressed in normal human peripheral blood mononuclear cells and up-regulated after in vitro stimulation and in patients with rheumatoid arthritis.
2004, Pubmed
Bartholome,
Membrane glucocorticoid receptors (mGCR) are expressed in normal human peripheral blood mononuclear cells and up-regulated after in vitro stimulation and in patients with rheumatoid arthritis.
2004,
Pubmed Binnig,
Atomic force microscope.
1986,
Pubmed Bridger,
Identification of an interchromosomal compartment by polymerization of nuclear-targeted vimentin.
1998,
Pubmed
,
Xenbase Buttgereit,
Rapid glucocorticoid effects on immune cells.
2002,
Pubmed Daneholt,
Assembly and transport of a premessenger RNP particle.
2001,
Pubmed Daneholt,
Pre-mRNP particles: From gene to nuclear pore.
1999,
Pubmed Dworetzky,
The effects of variations in the number and sequence of targeting signals on nuclear uptake.
1988,
Pubmed
,
Xenbase Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase Gao,
Expression of the glucocorticoid receptor gene is regulated during early embryogenesis of Xenopus laevis.
1994,
Pubmed
,
Xenbase Gerasimenko,
NAADP mobilizes Ca2+ from a thapsigargin-sensitive store in the nuclear envelope by activating ryanodine receptors.
2003,
Pubmed Golden,
Accumulation of individual pA+ RNAs during oogenesis of Xenopus laevis.
1980,
Pubmed
,
Xenbase Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed Greber,
Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus.
1995,
Pubmed Grote,
Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopy.
1995,
Pubmed Henderson,
Imaging ROMK1 inwardly rectifying ATP-sensitive K+ channel protein using atomic force microscopy.
1996,
Pubmed Hinterdorfer,
Detection and localization of individual antibody-antigen recognition events by atomic force microscopy.
1996,
Pubmed Hurst,
The evolutionary dynamics of eukaryotic gene order.
2004,
Pubmed Jäggi,
Modulation of nuclear pore topology by transport modifiers.
2003,
Pubmed
,
Xenbase Kastrup,
Nuclear envelope barrier leak induced by dexamethasone.
2006,
Pubmed
,
Xenbase Kienberger,
Visualization of single receptor molecules bound to human rhinovirus under physiological conditions.
2005,
Pubmed Kienberger,
Molecular recognition imaging and force spectroscopy of single biomolecules.
2006,
Pubmed Kiseleva,
Active nuclear pore complexes in Chironomus: visualization of transporter configurations related to mRNP export.
1998,
Pubmed Kurz,
Active and inactive genes localize preferentially in the periphery of chromosome territories.
1996,
Pubmed Lee,
Genomic gene clustering analysis of pathways in eukaryotes.
2003,
Pubmed Ludwig,
Hot spot formation in the nuclear envelope of oocytes in response to steroids.
2006,
Pubmed
,
Xenbase Maul,
The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
1977,
Pubmed Mazzanti,
Electrical dimension of the nuclear envelope.
2001,
Pubmed Müller,
Imaging purple membranes in aqueous solutions at sub-nanometer resolution by atomic force microscopy.
1995,
Pubmed Nevo,
A molecular switch between alternative conformational states in the complex of Ran and importin beta1.
2003,
Pubmed Oberleithner,
Endothelial cell swelling by aldosterone.
2003,
Pubmed Oberleithner,
Viewing the renal epithelium with the atomic force microscope.
1996,
Pubmed Oberleithner,
Visualizing life on biomembranes by atomic force microscopy.
1995,
Pubmed Oberleithner,
Imaging nuclear pores of aldosterone-sensitive kidney cells by atomic force microscopy.
1994,
Pubmed Panté,
Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm.
2002,
Pubmed
,
Xenbase Panté,
The nuclear pore complex.
1993,
Pubmed Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase Reich,
Scanning force microscopy in the applied biological sciences.
2001,
Pubmed Ribbeck,
The permeability barrier of nuclear pore complexes appears to operate via hydrophobic exclusion.
2002,
Pubmed Ris,
High-resolution field-emission scanning electron microscopy of nuclear pore complex.
1997,
Pubmed Rout,
Virtual gating and nuclear transport: the hole picture.
2003,
Pubmed Schäfer,
Aldosterone signaling pathway across the nuclear envelope.
2002,
Pubmed
,
Xenbase Schäfer,
Intracellular calcium: a prerequisite for aldosterone action.
2003,
Pubmed
,
Xenbase Schneider,
Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopy.
1998,
Pubmed Shahin,
Evidence for Ca2+- and ATP-sensitive peripheral channels in nuclear pore complexes.
2001,
Pubmed
,
Xenbase Shahin,
Glucocorticoids remodel nuclear envelope structure and permeability.
2005,
Pubmed
,
Xenbase Shahin,
The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure.
2006,
Pubmed Shahin,
Steroids dilate nuclear pores imaged with atomic force microscopy.
2005,
Pubmed
,
Xenbase Song,
Non-genomic glucocorticoid effects to provide the basis for new drug developments.
2006,
Pubmed Sperling,
Three-dimensional image reconstruction of large nuclear RNP (lnRNP) particles by automated electron tomography.
1997,
Pubmed Stehno-Bittel,
Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store.
1995,
Pubmed
,
Xenbase Stevens,
RNA transport from nucleus to cytoplasm in Chironomus salivary glands.
1966,
Pubmed Stoffler,
The nuclear pore complex: from molecular architecture to functional dynamics.
1999,
Pubmed Stoffler,
Calcium-mediated structural changes of native nuclear pore complexes monitored by time-lapse atomic force microscopy.
1999,
Pubmed
,
Xenbase Van Laethem,
Glucocorticoids alter the lipid and protein composition of membrane rafts of a murine T cell hybridoma.
2003,
Pubmed Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed Wang,
Conformational changes of the in situ nuclear pore complex.
1999,
Pubmed
,
Xenbase Wehling,
Specific, nongenomic actions of steroid hormones.
1997,
Pubmed
