Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Rod sensitivity and visual pigment concentration in Xenopus.
Engbretson GA, Witkovsky P.
???displayArticle.abstract???
Xenopus larvae were raised on a vitamin A-free diet under constant illumination until their visual pigment content had decreased to between 8% of normal and an undetectably low level. After the intramuscular injection of 2.1 X 10(13-2.1 X 10(16) molecules of [3H]vitamin A, ocular tissue showed a rapid rate of uptake of label which reached a maximum level of incorporation by 48 h. Light-microscopic autoradiography revealed that the retinal uptake of label was concentrated within the receptor outer segments. Spectral transmissivity measurements at various times after injection were made upon intact retinas and upon digitonin extracts. They showed that visual pigment with a lambdamax of 504 nm was formed in the retina and that the amount formed was a function of incubation time and the magnitude of the dose administered. Electrophysiological measures of photoreceptor light responses were obtained from the PIII component of the electroretinogram, isolated with aspartate. The quantal flux required to elicit a criterion response was determined and related to the fraction of visual pigment present. The results showed that rod sensitivity varied linearly with the probability of quantal absorption.
Boynton,
Visual adaptation in monkey cones: recordings of late receptor potentials.
1970, Pubmed
Boynton,
Visual adaptation in monkey cones: recordings of late receptor potentials.
1970,
Pubmed Bridges,
The visual pigment and vitamin A of Xenopus laevis embryos, larvae and adults.
1977,
Pubmed
,
Xenbase Bridges,
Effects of light and darkness on the visual pigments of amphibian tadpoles.
1974,
Pubmed
,
Xenbase Bridges,
A method for preparing stable digitonin solutions for visual pigment extraction.
1977,
Pubmed Brin,
Rhodopsin photoproducts and rod sensitivity in the skate retina.
1977,
Pubmed Cervetto,
Electrical responses of rods in the retina of Bufo marinus.
1977,
Pubmed Dowling,
Adaptation in skate photoreceptors.
1972,
Pubmed Dowling,
VITAMIN A DEFICIENCY AND NIGHT BLINDNESS.
1958,
Pubmed Dowling,
THE BIOLOGICAL FUNCTION OF VITAMIN A ACID.
1960,
Pubmed Dowling,
Visual adaptation in the retina of the skate.
1970,
Pubmed Eakin,
Localization of vitamin A in the eye of a pulmonate snail.
1968,
Pubmed Ernst,
The effects of rhodopsin decomposition on P3 responses of isolated rat retinae.
1972,
Pubmed Fain,
Voltage signal of photoreceptors at visual threshold.
1977,
Pubmed Fain,
Sensitivity of toad rods: Dependence on wave-length and background illumination.
1976,
Pubmed Grabowski,
Intracellular recordings of rod responses during dark-adaptation.
1975,
Pubmed Hall,
Reduction of the retinal-opsin linkage in isolated frog retinas.
1976,
Pubmed Harris,
Vitamin A deprivation and Drosophila photopigments.
1977,
Pubmed Hood,
Light adaptation of the receptors: increment threshold functions for the frog's rods and cones.
1975,
Pubmed Hood,
Dark adaptation of the frog's rods.
1973,
Pubmed Kleinschmidt,
Intracellular recordings from gecko photoreceptors during light and dark adaptation.
1975,
Pubmed Korenbrot,
Dark ionic flux and the effects of light in isolated rod outer segments.
1972,
Pubmed Kühn,
Phosphorylation of rhodopsin as a possible mechanism of adaptation.
1977,
Pubmed Normann,
Control of retinal sensitivity. I. Light and dark adaptation of vertebrate rods and cones.
1974,
Pubmed O'BRIEN,
Vision and resolution in the central retina.
1951,
Pubmed Ripps,
Rhodopsin and visual threshold in retinitis pigmentosa.
1978,
Pubmed RUSHTON,
VISUAL ADAPTATION.
1965,
Pubmed RUSHTON,
Rhodopsin measurement and dark-adaptation in a subject deficient in cone vision.
1961,
Pubmed Sillman,
Studies on the mass receptor potential of the isolated frog retina. I. General properties of the response.
1969,
Pubmed Witkovsky,
Action spectra and adaptation properties of carp photoreceptors.
1973,
Pubmed Witkovsky,
Slow PIII component of the carp electroretinogram.
1975,
Pubmed Witkovsky,
Photoreceptor thresholds and visual pigment levels in normal and vitamin A-deprived Xenopus tadpoles.
1976,
Pubmed
,
Xenbase
