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Gene
2008 Jan 31;4081-2:133-45. doi: 10.1016/j.gene.2007.10.029.
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Alternative polyadenylation signals in the 3' non-coding region of a voltage-gated potassium channel gene are major determinants of mRNA isoform expression.
Jang GM, Tanaka BS, Gutman GA, Goldin AL, Semler BL.
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We investigated the role of the 3' non-coding region of a mouse voltage-gated potassium channel mRNA (mKv1.4 mRNA) in post-transcriptional regulation of gene expression. In contrast to an earlier report from studies carried out in Xenopus oocytes, we found that 3' non-coding region sequences of mKv1.4 mRNAs did not significantly affect expression of a heterologous reporter RNA in vitro or in mammalian cells/cell lines. Instead, our data revealed a possible role for alternative polyadenylation mediated by distinct determinants approximately 0.2 kb and approximately 1.2 kb downstream of the Kv1.4 coding region. The use of the downstream polyadenylation signal correlated with the synthesis of a larger Kv1.4 mRNA isoform that was more abundantly expressed than the smaller mRNA species, whose expression was regulated by the upstream polyadenylation signal. Our results suggest that the relative strengths of the polyadenylation signals are major determinants of overall Kv1.4 mRNA abundance in cells.
Bakheet,
ARED: human AU-rich element-containing mRNA database reveals an unexpectedly diverse functional repertoire of encoded proteins.
2001, Pubmed
Bakheet,
ARED: human AU-rich element-containing mRNA database reveals an unexpectedly diverse functional repertoire of encoded proteins.
2001,
Pubmed Bakheet,
ARED 2.0: an update of AU-rich element mRNA database.
2003,
Pubmed Berget,
Exon recognition in vertebrate splicing.
1995,
Pubmed Boelens,
The human U1 snRNP-specific U1A protein inhibits polyadenylation of its own pre-mRNA.
1993,
Pubmed
,
Xenbase Calvo,
Strange bedfellows: polyadenylation factors at the promoter.
2003,
Pubmed Chen,
AU-rich elements: characterization and importance in mRNA degradation.
1995,
Pubmed Edwalds-Gilbert,
Alternative poly(A) site selection in complex transcription units: means to an end?
1997,
Pubmed Goldin,
Expression of ion channels by injection of mRNA into Xenopus oocytes.
1991,
Pubmed
,
Xenbase Gunderson,
The human U1A snRNP protein regulates polyadenylation via a direct interaction with poly(A) polymerase.
1994,
Pubmed Jang,
Structurally distinct elements mediate internal ribosome entry within the 5'-noncoding region of a voltage-gated potassium channel mRNA.
2004,
Pubmed Phillips,
U1A inhibits cleavage at the immunoglobulin M heavy-chain secretory poly(A) site by binding between the two downstream GU-rich regions.
2004,
Pubmed Phillips,
Regulation of nuclear poly(A) addition controls the expression of immunoglobulin M secretory mRNA.
2001,
Pubmed Proudfoot,
How RNA polymerase II terminates transcription in higher eukaryotes.
1989,
Pubmed Takagaki,
The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.
1996,
Pubmed Wymore,
Characterization of the transcription unit of mouse Kv1.4, a voltage-gated potassium channel gene.
1996,
Pubmed
,
Xenbase Wymore,
Genomic organization, nucleotide sequence, biophysical properties, and localization of the voltage-gated K+ channel gene KCNA4/Kv1.4 to mouse chromosome 2/human 11p14 and mapping of KCNC1/Kv3.1 to mouse 7/human 11p14.3-p15.2 and KCNA1/Kv1.1 to human 12p13.
1994,
Pubmed Xu,
Developmental analysis reveals mismatches in the expression of K+ channel alpha subunits and voltage-gated K+ channel currents in rat ventricular myocytes.
1996,
Pubmed Zhao,
Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.
1999,
Pubmed
