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J Cell Physiol
2008 Dec 01;2173:626-31. doi: 10.1002/jcp.21531.
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Cyclin E in centrosome duplication and reduplication in sea urchin zygotes.
Schnackenberg BJ, Marzluff WF, Sluder G.
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When protein synthesis is completely blocked from before fertilization, the sea urchin zygote arrests in first S phase and the paternal centrosome reduplicates multiple times. However, when protein synthesis is blocked starting in prophase of first mitosis, the zygote divides and the blastomeres arrest in a G1-like state. The centrosome inherited from this mitosis duplicates only once in each blastomere for reasons that are not understood. The late G1 rise in cyclin E/cdk2 kinase activity initiates centrosome duplication in mammalian cells and its activity is needed for centrosome duplication in Xenopus egg extracts. Since the half-time for cyclin E turnover is normally approximately 1 h in sea urchin zygotes, the different behaviors of centrosomes during G1 and S phase arrests could be due to differential losses of cyclin E and its associated kinase activities at these two arrest points. To better understand the mechanisms that limit centrosome duplication, we characterize the levels of cyclin E and its associated kinase activity at the S phase and G1 arrest points. We first demonstrate that cyclin E/cdk2 kinase activity is required for centrosome duplication and reduplication in sea urchin zygotes. Next we find that cyclin E levels and cyclin E/cdk2 kinase activities are both constitutively and equivalently elevated during both the S phase and G1 arrests. This indicates that centrosome duplication during the G1 arrest is limited by a block to reduplication under conditions permissive for duplication. The cytoplasmic conditions of S phase, however, abrogate this block to reduplication.
Alessi,
The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity.
1998, Pubmed
Alessi,
The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity.
1998,
Pubmed Balczon,
Dissociation of centrosome replication events from cycles of DNA synthesis and mitotic division in hydroxyurea-arrested Chinese hamster ovary cells.
1995,
Pubmed Carrano,
SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27.
1999,
Pubmed Chevalier,
Xenopus cyclin E, a nuclear phosphoprotein, accumulates when oocytes gain the ability to initiate DNA replication.
1996,
Pubmed
,
Xenbase Gard,
Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle.
1990,
Pubmed
,
Xenbase Habedanck,
The Polo kinase Plk4 functions in centriole duplication.
2005,
Pubmed Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase Hinchcliffe,
Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase Hinchcliffe,
The coordination of centrosome reproduction with nuclear events of the cell cycle in the sea urchin zygote.
1998,
Pubmed Hinchcliffe,
Two for two: Cdk2 and its role in centrosome doubling.
2002,
Pubmed Howe,
A developmental timer regulates degradation of cyclin E1 at the midblastula transition during Xenopus embryogenesis.
1996,
Pubmed
,
Xenbase Kellogg,
The centrosome and cellular organization.
1994,
Pubmed Knoblich,
Cyclin E controls S phase progression and its down-regulation during Drosophila embryogenesis is required for the arrest of cell proliferation.
1994,
Pubmed Kuriyama,
Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy.
1981,
Pubmed Lacey,
Cyclin-dependent kinase control of centrosome duplication.
1999,
Pubmed
,
Xenbase Lingle,
The role of the centrosome in the development of malignant tumors.
2000,
Pubmed Longo,
The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata.
1968,
Pubmed Marti,
Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation.
1999,
Pubmed Matsumoto,
Calcium, calmodulin, and CaMKII requirement for initiation of centrosome duplication in Xenopus egg extracts.
2002,
Pubmed
,
Xenbase Matsumoto,
Cyclin-dependent kinase 2 (Cdk2) is required for centrosome duplication in mammalian cells.
1999,
Pubmed Mazia,
The Multiplicity of the Mitotic Centers and the Time-Course of Their Duplication and Separation.
1960,
Pubmed Nakayama,
Targeted disruption of Skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication.
2000,
Pubmed Rempel,
Maternal Xenopus Cdk2-cyclin E complexes function during meiotic and early embryonic cell cycles that lack a G1 phase.
1995,
Pubmed
,
Xenbase Salisbury,
Centrin-2 is required for centriole duplication in mammalian cells.
2002,
Pubmed Sauer,
Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis.
1995,
Pubmed Schatten,
The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization.
1994,
Pubmed Schnackenberg,
Cyclin E/Cdk2 is required for sperm maturation, but not DNA replication, in early sea urchin embryos.
2007,
Pubmed Schnackenberg,
Novel localization and possible functions of cyclin E in early sea urchin development.
2002,
Pubmed Singer,
Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.
1999,
Pubmed Sluder,
Experimental analysis of the reproduction of spindle poles.
1985,
Pubmed Sluder,
Protein synthesis and the cell cycle: centrosome reproduction in sea urchin eggs is not under translational control.
1990,
Pubmed Sluder,
Centriole number and the reproductive capacity of spindle poles.
1985,
Pubmed Sumerel,
Cyclin E and its associated cdk activity do not cycle during early embryogenesis of the sea Urchin.
2001,
Pubmed Tsou,
Mechanism limiting centrosome duplication to once per cell cycle.
2006,
Pubmed
,
Xenbase Vacquier,
Sea urchin eggs release protease activity at fertilization.
1972,
Pubmed Wong,
Centrosome number is controlled by a centrosome-intrinsic block to reduplication.
2003,
Pubmed Zhang,
p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase.
1995,
Pubmed
