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???displayArticle.abstract??? SPARC is a multifunctional matricellular glycoprotein with complex, transient tissue distribution during embryonic development. In Xenopus laevis embryos, zygotic activation of SPARC is first detected during late gastrulation, undergoing rapid changes in its spatiotemporal distribution throughout organogenesis. Injections of anti-sense Xenopus SPARC morpholinos (XSMOs) into 2- and 4-cell embryos led to a dose-dependent dissociation of embryos during neurula and tailbud stages of development. Animal cap explants derived from XSMO-injected embryos also dissociated, resulting in the formation of amorphous ciliated microspheres. At low doses of XSMOs, lens cataracts were formed, phenocopying that observed in Sparc-null mice. At XSMOs concentrations that did not result in a loss of axial tissue integrity, adhesion between myotomes at intersomitic borders was compromised with a reduction in SPARC concentration. The combined data suggest a critical requirement for SPARC during post-gastrula development in Xenopus embryos and that SPARC, directly or indirectly, promotes cell-cell adhesion in vivo.
Atorrasagasti,
Overexpression of SPARC obliterates the in vivo tumorigenicity of human hepatocellular carcinoma cells.
2010, Pubmed
Atorrasagasti,
Overexpression of SPARC obliterates the in vivo tumorigenicity of human hepatocellular carcinoma cells.
2010,
Pubmed Barker,
SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity.
2005,
Pubmed Bradshaw,
SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury.
2001,
Pubmed Brekken,
SPARC, a matricellular protein: at the crossroads of cell-matrix communication.
2001,
Pubmed Brown,
Effects of hyaluronan and SPARC on fibroproliferative events assessed in an in vitro bladder acellular matrix model.
2006,
Pubmed Cardellini,
Tight junctions in early amphibian development: detection of junctional cingulin from the 2-cell stage and its localization at the boundary of distinct membrane domains in dividing blastomeres in low calcium.
1996,
Pubmed
,
Xenbase Clark,
A prototypic matricellular protein in the tumor microenvironment--where there's SPARC, there's fire.
2008,
Pubmed Damjanovski,
Ectopic expression of SPARC in Xenopus embryos interferes with tissue morphogenesis: identification of a bioactive sequence in the C-terminal EF hand.
1997,
Pubmed
,
Xenbase Damjanovski,
Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein.
1992,
Pubmed
,
Xenbase Damjanovski,
Transient expression of SPARC in the dorsal axis of early Xenopus embryos: correlation with calcium-dependent adhesion and electrical coupling.
1994,
Pubmed
,
Xenbase Dawid,
Mesoderm induction.
1991,
Pubmed
,
Xenbase Gilmour,
Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.
1998,
Pubmed Giudici,
Mapping of SPARC/BM-40/osteonectin-binding sites on fibrillar collagens.
2008,
Pubmed Greiling,
Absence of SPARC leads to impaired lens circulation.
2009,
Pubmed Hayes,
The distribution of intercellular junctions in the developing myotomes of the clawed toad.
1975,
Pubmed
,
Xenbase Heasman,
Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.
2000,
Pubmed
,
Xenbase Heasman,
Morpholino oligos: making sense of antisense?
2002,
Pubmed
,
Xenbase Henry,
Characterizing gene expression during lens formation in Xenopus laevis: evaluating the model for embryonic lens induction.
2002,
Pubmed
,
Xenbase Hens,
Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis.
1995,
Pubmed
,
Xenbase Hohenester,
Structural basis of sequence-specific collagen recognition by SPARC.
2008,
Pubmed Huynh,
Interaction between SPARC and tubulin in Xenopus.
2004,
Pubmed
,
Xenbase Kang,
Sparc protein is required for normal growth of zebrafish otoliths.
2008,
Pubmed Kawasaki,
Evolutionary genetics of vertebrate tissue mineralization: the origin and evolution of the secretory calcium-binding phosphoprotein family.
2006,
Pubmed Kawasaki,
Gene duplication and the evolution of vertebrate skeletal mineralization.
2007,
Pubmed Koehler,
Molecular evolution of SPARC: absence of the acidic module and expression in the endoderm of the starlet sea anemone, Nematostella vectensis.
2009,
Pubmed Kragtorp,
Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development.
2006,
Pubmed
,
Xenbase Lane,
The biology of SPARC, a protein that modulates cell-matrix interactions.
1994,
Pubmed Martinek,
Is SPARC an evolutionarily conserved collagen chaperone?
2007,
Pubmed Mason,
Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000.
1986,
Pubmed McClung,
SPARC upregulates MT1-MMP expression, MMP-2 activation, and the secretion and cleavage of galectin-3 in U87MG glioma cells.
2007,
Pubmed Motamed,
Inhibition of PDGF-stimulated and matrix-mediated proliferation of human vascular smooth muscle cells by SPARC is independent of changes in cell shape or cyclin-dependent kinase inhibitors.
2002,
Pubmed Motamed,
Fibroblast growth factor receptor-1 mediates the inhibition of endothelial cell proliferation and the promotion of skeletal myoblast differentiation by SPARC: a role for protein kinase A.
2003,
Pubmed Murphy-Ullrich,
The de-adhesive activity of matricellular proteins: is intermediate cell adhesion an adaptive state?
2001,
Pubmed Nie,
IFATS collection: Combinatorial peptides identify alpha5beta1 integrin as a receptor for the matricellular protein SPARC on adipose stromal cells.
2008,
Pubmed Nie,
SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling.
2009,
Pubmed Norose,
Lenses of SPARC-null mice exhibit an abnormal cell surface-basement membrane interface.
2000,
Pubmed Norose,
SPARC deficiency leads to early-onset cataractogenesis.
1998,
Pubmed Nutt,
Comparison of morpholino based translational inhibition during the development of Xenopus laevis and Xenopus tropicalis.
2001,
Pubmed
,
Xenbase Pottgiesser,
Changes in calcium and collagen IV binding caused by mutations in the EF hand and other domains of extracellular matrix protein BM-40 (SPARC, osteonectin).
1994,
Pubmed Purcell,
Developmental anomalies of Xenopus embryos following microinjection of SPARC antibodies.
1993,
Pubmed
,
Xenbase Raines,
The extracellular glycoprotein SPARC interacts with platelet-derived growth factor (PDGF)-AB and -BB and inhibits the binding of PDGF to its receptors.
1992,
Pubmed Rentz,
SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts.
2007,
Pubmed Rotllant,
Sparc (Osteonectin) functions in morphogenesis of the pharyngeal skeleton and inner ear.
2008,
Pubmed Sage,
Distribution of the calcium-binding protein SPARC in tissues of embryonic and adult mice.
1989,
Pubmed Said,
Secreted protein acidic and rich in cysteine (SPARC) inhibits integrin-mediated adhesion and growth factor-dependent survival signaling in ovarian cancer.
2007,
Pubmed Weaver,
The copper binding domain of SPARC mediates cell survival in vitro via interaction with integrin beta1 and activation of integrin-linked kinase.
2008,
Pubmed Winklbauer,
Conditions for fibronectin fibril formation in the early Xenopus embryo.
1998,
Pubmed
,
Xenbase Yan,
Alterations in the lens capsule contribute to cataractogenesis in SPARC-null mice.
2002,
Pubmed Yan,
SPARC, a matricellular glycoprotein with important biological functions.
1999,
Pubmed
