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J Biol Chem
2011 Nov 18;28646:39760-7. doi: 10.1074/jbc.M111.289090.
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Membrane depolarization increases membrane PtdIns(4,5)P2 levels through mechanisms involving PKC βII and PI4 kinase.
Chen X, Zhang X, Jia C, Xu J, Gao H, Zhang G, Du X, Zhang H.
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In a previous study, we showed that membrane depolarization induced elevation of membrane phosphatidylinositol 4,5-bisphosphates (PtdIns(4,5)P(2), also known as PIP(2)) and subsequently increased the KCNQ2/Q3 currents expressed in Xenopus oocytes through increased PI4 kinase activity. In this study, the underlying mechanism for this depolarization-induced enhancement of PIP(2) synthesis was further investigated. Our results indicate that activation of protein kinase C (PKC) isozyme βII was responsible for the enhanced PIP(2) synthesis. We found that phorbol-12-myristate, 13-acetate (PMA), an activator of PKC, mimicked the effects of the membrane depolarization by increasing KCNQ2/Q3 activity, elevating membrane PIP(2) levels and increasing activity of PI4 kinase β. Furthermore, membrane depolarization enhanced PKC activity. The effects of both depolarization and PMA were blocked by a PKC inhibitor or PI4 kinase β RNA interference. Further results demonstrate that the depolarization selectively activated the PKC βII isoform and enhanced its interaction with PI4 kinase β. These results reveal that the depolarization-induced elevation of membrane PIP(2) is through activation of PKC and the subsequent increased activity of PI4 kinase β.
Akiyama,
Genistein, a specific inhibitor of tyrosine-specific protein kinases.
1987, Pubmed
Akiyama,
Genistein, a specific inhibitor of tyrosine-specific protein kinases.
1987,
Pubmed Apgar,
Activation of protein kinase C in rat basophilic leukemia cells stimulates increased production of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: correlation with actin polymerization.
1995,
Pubmed Araya,
Dihydropyridine receptors as voltage sensors for a depolarization-evoked, IP3R-mediated, slow calcium signal in skeletal muscle cells.
2003,
Pubmed Audigier,
[Diffuse colon linitis secondary to a gastric adenocarcinoma. Detected during diarrhea treated with octreotide].
1998,
Pubmed Babwah,
Protein kinase C isoform-specific differences in the spatial-temporal regulation and decoding of metabotropic glutamate receptor1a-stimulated second messenger responses.
2003,
Pubmed Becker,
Isoenzyme-specific translocation of protein kinase C (PKC)betaII and not PKCbetaI to a juxtanuclear subset of recycling endosomes: involvement of phospholipase D.
2004,
Pubmed Ben-Chaim,
The M2 muscarinic G-protein-coupled receptor is voltage-sensitive.
2003,
Pubmed
,
Xenbase Ben-Chaim,
Movement of 'gating charge' is coupled to ligand binding in a G-protein-coupled receptor.
2006,
Pubmed
,
Xenbase Billups,
Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.
2006,
Pubmed Cárdenas,
Depolarization of skeletal muscle cells induces phosphorylation of cAMP response element binding protein via calcium and protein kinase Calpha.
2004,
Pubmed Cenni,
Regulation of novel protein kinase C epsilon by phosphorylation.
2002,
Pubmed Corbalán-García,
The C2 domains of classical/conventional PKCs are specific PtdIns(4,5)P(2)-sensing domains.
2007,
Pubmed Di Paolo,
Extended double-dosage HBV vaccination after liver transplantation is ineffective, in the absence of lamivudine and prior wash-out of human Hepatitis B immunoglobulins.
2006,
Pubmed Du,
Characteristic interactions with phosphatidylinositol 4,5-bisphosphate determine regulation of kir channels by diverse modulators.
2004,
Pubmed
,
Xenbase Eltit,
Slow calcium signals after tetanic electrical stimulation in skeletal myotubes.
2004,
Pubmed Etkovitz,
Role of PI3-kinase and PI4-kinase in actin polymerization during bovine sperm capacitation.
2007,
Pubmed Evans,
The state of the union: the cell biology of fertilization.
2002,
Pubmed Gamper,
Target-specific PIP(2) signalling: how might it work?
2007,
Pubmed Gonzales,
Nuclear phosphoinositide kinases and inositol phospholipids.
2006,
Pubmed Goueli,
A novel and simple method to assay the activity of individual protein kinases in a crude tissue extract.
1995,
Pubmed Guerrero-Valero,
The C2 domains of classical PKCs are specific PtdIns(4,5)P2-sensing domains with different affinities for membrane binding.
2007,
Pubmed Hamplová,
Transient upregulation of protein kinase C in pressure-overloaded neonatal rat myocardium.
2010,
Pubmed Hilgemann,
The complex and intriguing lives of PIP2 with ion channels and transporters.
2001,
Pubmed Johnson,
Progesterone acts through protein kinase C to remodel the cytoplasm as the amphibian oocyte becomes the fertilization-competent egg.
1997,
Pubmed
,
Xenbase Kline,
The wave of activation current in the Xenopus egg.
1985,
Pubmed
,
Xenbase Krauss,
Phosphoinositides: regulators of membrane traffic and protein function.
2007,
Pubmed Lee,
Protein kinase C-beta gene variants, pathway activation, and enzastaurin activity in lung cancer.
2010,
Pubmed Li,
Regulation of Kv7 (KCNQ) K+ channel open probability by phosphatidylinositol 4,5-bisphosphate.
2005,
Pubmed Maasch,
Protein kinase calpha targeting is regulated by temporal and spatial changes in intracellular free calcium concentration [Ca(2+)](i).
2000,
Pubmed Margolis,
EGF induces tyrosine phosphorylation of phospholipase C-II: a potential mechanism for EGF receptor signaling.
1989,
Pubmed Martinez-Pinna,
Direct voltage control of signaling via P2Y1 and other Galphaq-coupled receptors.
2005,
Pubmed McLaughlin,
PIP(2) and proteins: interactions, organization, and information flow.
2002,
Pubmed Mortier,
Nuclear speckles and nucleoli targeting by PIP2-PDZ domain interactions.
2005,
Pubmed Nasuhoglu,
Modulation of cardiac PIP2 by cardioactive hormones and other physiologically relevant interventions.
2002,
Pubmed Powell,
IP(3) receptor function and localization in myotubes: an unexplored Ca(2+) signaling pathway in skeletal muscle.
2001,
Pubmed Rajagopal,
Protein kinase C isozyme-specific potentiation of expressed Ca v 2.3 currents by acetyl-beta-methylcholine and phorbol-12-myristate, 13-acetate.
2008,
Pubmed
,
Xenbase Rameh,
A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate.
1997,
Pubmed Snow,
Fertilization stimulates an increase in inositol trisphosphate and inositol lipid levels in Xenopus eggs.
1996,
Pubmed
,
Xenbase Steinberg,
Structural basis of protein kinase C isoform function.
2008,
Pubmed Stith,
sn-1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis.
1997,
Pubmed
,
Xenbase Stumpo,
Protein kinase C isozyme distribution and down-regulation in relation to insulin-stimulated c-fos induction.
1994,
Pubmed Suh,
Regulation of ion channels by phosphatidylinositol 4,5-bisphosphate.
2005,
Pubmed Suh,
PIP2 is a necessary cofactor for ion channel function: how and why?
2008,
Pubmed Suh,
Recovery from muscarinic modulation of M current channels requires phosphatidylinositol 4,5-bisphosphate synthesis.
2002,
Pubmed Tolias,
Pathways for phosphoinositide synthesis.
1999,
Pubmed Winks,
Relationship between membrane phosphatidylinositol-4,5-bisphosphate and receptor-mediated inhibition of native neuronal M channels.
2005,
Pubmed Zhang,
PIP(2) activates KCNQ channels, and its hydrolysis underlies receptor-mediated inhibition of M currents.
2003,
Pubmed
,
Xenbase Zhang,
Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons.
2004,
Pubmed Zhang,
Depolarization increases phosphatidylinositol (PI) 4,5-bisphosphate level and KCNQ currents through PI 4-kinase mechanisms.
2010,
Pubmed
,
Xenbase Zhao,
Molecular basis for genistein-induced inhibition of Kir2.3 currents.
2008,
Pubmed
,
Xenbase
