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Arteriovenous (AV) differentiation is a critical step during blood vessel formation and stabilization. Defects in arterial or venous fate lead to inappropriate fusion of vessels, resulting in damaging arteriovenous shunts. While many studies have unraveled the molecular underpinnings that drive AV fate, surprisingly, the spatiotemporal emergence of arteries and veins in mammalian embryos remains unknown. Here, we examine artery and vein specification and differentiation during vasculogenesis. We show that the first intraembryonic vessels formed are arteries, which differentiate in a stepwise manner. By contrast, veins emerge later, progressively forming after embryonic turning. In addition, we demonstrate that hemodynamic flow is not required for arterial specification, but is required for maintenance of select arterial markers. Together, our results provide a first spatiotemporal analysis of mammalian AV cell fate establishment and anatomy, as well as a delineation of a molecular toolkit for analysis of arteries and veins during early vessel development.
Aird,
Phenotypic heterogeneity of the endothelium: II. Representative vascular beds.
2007, Pubmed
Aird,
Phenotypic heterogeneity of the endothelium: II. Representative vascular beds.
2007,
Pubmed Andersson,
Differential global gene expression response patterns of human endothelium exposed to shear stress and intraluminal pressure.
2005,
Pubmed Blum,
Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo.
2008,
Pubmed Drake,
Vasculogenesis in the day 6.5 to 9.5 mouse embryo.
2000,
Pubmed Ebong,
Flow regulates intercellular communication in HAEC by assembling functional Cx40 and Cx37 gap junctional channels.
2006,
Pubmed Eichmann,
Vascular development: from precursor cells to branched arterial and venous networks.
2005,
Pubmed Ema,
Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors.
2006,
Pubmed Herbert,
Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation.
2009,
Pubmed Herzog,
Segregation of arterial and venous markers in subpopulations of blood islands before vessel formation.
2005,
Pubmed Jones,
Separating genetic and hemodynamic defects in neuropilin 1 knockout embryos.
2008,
Pubmed Jones,
Measuring hemodynamic changes during mammalian development.
2004,
Pubmed Jones,
Dynamic in vivo imaging of postimplantation mammalian embryos using whole embryo culture.
2002,
Pubmed Kim,
Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis.
2008,
Pubmed Lawson,
Notch signaling is required for arterial-venous differentiation during embryonic vascular development.
2001,
Pubmed Lawson,
sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation.
2002,
Pubmed le Noble,
Flow regulates arterial-venous differentiation in the chick embryo yolk sac.
2004,
Pubmed Lucitti,
Vascular remodeling of the mouse yolk sac requires hemodynamic force.
2007,
Pubmed McGrath,
Circulation is established in a stepwise pattern in the mammalian embryo.
2003,
Pubmed McKinney,
Chapter 4. Using the zebrafish to study vessel formation.
2008,
Pubmed Reese,
Negative regulation of midline vascular development by the notochord.
2004,
Pubmed Risau,
Vasculogenesis.
1995,
Pubmed Rocha,
Molecular differentiation and specialization of vascular beds.
2009,
Pubmed Shalaby,
Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice.
1995,
Pubmed Strilić,
The molecular basis of vascular lumen formation in the developing mouse aorta.
2009,
Pubmed Villasenor,
Biphasic Ngn3 expression in the developing pancreas.
2008,
Pubmed
,
Xenbase Walls,
Three-dimensional analysis of vascular development in the mouse embryo.
2008,
Pubmed Wang,
Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4.
1998,
Pubmed Xu,
Blood vessel tubulogenesis requires Rasip1 regulation of GTPase signaling.
2011,
Pubmed
,
Xenbase Yamaguchi,
flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors.
1993,
Pubmed You,
Suppression of Notch signalling by the COUP-TFII transcription factor regulates vein identity.
2005,
Pubmed Zhong,
Gridlock signalling pathway fashions the first embryonic artery.
2001,
Pubmed
