XB-ART-45402
Methods Mol Biol
2012 Jan 01;884:41-51. doi: 10.1007/978-1-61779-848-1_3.
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In vivo functional analysis of transcription factor: response element interaction using transgenic Xenopus laevis.
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Analysis of transcription factor-target interactions in vivo is important to the study of transcriptional regulation of gene expression. A key experiment involves analysis of the functional interaction between a trans-acting factor and its corresponding cis-acting element in the context of a target promoter in vivo. We describe a method for this analysis in transgenic Xenopus tadpoles in which expression of the trans-acting factor is knocked down using an shRNA-mediated approach.
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