Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2012 Oct 12;28742:35333-35340. doi: 10.1074/jbc.M112.385393.
Show Gene links
Show Anatomy links
Amer2 protein interacts with EB1 protein and adenomatous polyposis coli (APC) and controls microtubule stability and cell migration.
Pfister AS, Hadjihannas MV, Röhrig W, Schambony A, Behrens J.
???displayArticle.abstract??? EB1 is key factor in the organization of the microtubule cytoskeleton by binding to the plus-ends of microtubules and serving as a platform for a number of interacting proteins (termed +TIPs) that control microtubule dynamics. Together with its direct binding partner adenomatous polyposis coli (APC), EB1 can stabilize microtubules. Here, we show that Amer2 (APC membrane recruitment 2), a previously identified membrane-associated APC-binding protein, is a direct interaction partner of EB1 and acts as regulator of microtubule stability together with EB1. Amer2 binds to EB1 via specific (S/T)xIP motifs and recruits it to the plasma membrane. Coexpression of Amer2 and EB1 generates stabilized microtubules at the plasma membrane, whereas knockdown of Amer2 leads to destabilization of microtubules. Knockdown of Amer2, APC, or EB1 reduces cell migration, and morpholino-mediated down-regulation of Xenopus Amer2 blocks convergent extension cell movements, suggesting that the Amer2-EB1-APC complex regulates cell migration by altering microtubule stability.
Akhmanova,
Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
2008, Pubmed
Akhmanova,
Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
2008,
Pubmed Askham,
Regulation and function of the interaction between the APC tumour suppressor protein and EB1.
2000,
Pubmed Barth,
Dissecting interactions between EB1, microtubules and APC in cortical clusters at the plasma membrane.
2002,
Pubmed Behrens,
Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta.
1998,
Pubmed
,
Xenbase Boutet,
The WTX/AMER1 gene family: evolution, signature and function.
2010,
Pubmed Grohmann,
AMER1 regulates the distribution of the tumor suppressor APC between microtubules and the plasma membrane.
2007,
Pubmed Honnappa,
An EB1-binding motif acts as a microtubule tip localization signal.
2009,
Pubmed Kita,
Adenomatous polyposis coli on microtubule plus ends in cell extensions can promote microtubule net growth with or without EB1.
2006,
Pubmed Komarova,
Mammalian end binding proteins control persistent microtubule growth.
2009,
Pubmed Krieghoff,
Nucleo-cytoplasmic distribution of beta-catenin is regulated by retention.
2006,
Pubmed Kroboth,
Lack of adenomatous polyposis coli protein correlates with a decrease in cell migration and overall changes in microtubule stability.
2007,
Pubmed Lansbergen,
CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta.
2006,
Pubmed Li,
Beyond polymer polarity: how the cytoskeleton builds a polarized cell.
2008,
Pubmed Louie,
Adenomatous polyposis coli and EB1 localize in close proximity of the mother centriole and EB1 is a functional component of centrosomes.
2004,
Pubmed Lustig,
Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors.
2002,
Pubmed Major,
Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling.
2007,
Pubmed
,
Xenbase Mimori-Kiyosue,
The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.
2000,
Pubmed
,
Xenbase Nakamura,
Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization.
2001,
Pubmed Pfister,
Amer2 protein is a novel negative regulator of Wnt/β-catenin signaling involved in neuroectodermal patterning.
2012,
Pubmed
,
Xenbase Piehl,
Organization and dynamics of growing microtubule plus ends during early mitosis.
2003,
Pubmed Piperno,
Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.
1987,
Pubmed Polakis,
Wnt signaling and cancer.
2000,
Pubmed Rivera,
An X chromosome gene, WTX, is commonly inactivated in Wilms tumor.
2007,
Pubmed Salaycik,
Quantification of microtubule nucleation, growth and dynamics in wound-edge cells.
2005,
Pubmed Su,
APC binds to the novel protein EB1.
1995,
Pubmed Tanneberger,
Amer1/WTX couples Wnt-induced formation of PtdIns(4,5)P2 to LRP6 phosphorylation.
2011,
Pubmed Tirnauer,
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules.
2002,
Pubmed
,
Xenbase Unterseher,
Paraxial protocadherin coordinates cell polarity during convergent extension via Rho A and JNK.
2004,
Pubmed
,
Xenbase Wen,
EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration.
2004,
Pubmed Zumbrunn,
Binding of the adenomatous polyposis coli protein to microtubules increases microtubule stability and is regulated by GSK3 beta phosphorylation.
2001,
Pubmed
