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Force-dependent detachment of kinesin-2 biases track switching at cytoskeletal filament intersections.
Schroeder HW, Hendricks AG, Ikeda K, Shuman H, Rodionov V, Ikebe M, Goldman YE, Holzbaur EL.
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Intracellular trafficking of organelles often involves cytoskeletal track switching. Organelles such as melanosomes are transported by multiple motors including kinesin-2, dynein, and myosin-V, which drive switching between microtubules and actin filaments during dispersion and aggregation. Here, we used optical trapping to determine the unitary and ensemble forces of kinesin-2, and to reconstitute cargo switching at cytoskeletal intersections in a minimal system with kinesin-2 and myosin-V motors bound to beads. Single kinesin-2 motors exerted forces up to ∼5 pN, similar to kinesin-1. However, kinesin-2 motors were more likely to detach at submaximal forces, and the duration of force maintenance was short as compared to kinesin-1. In multimotor assays, force increased with kinesin-2 density but was not affected by the presence of myosin-V. In crossed filament assays, switching frequencies of motor-bound beads were dependent on the starting track. At equal average forces, beads tended to switch from microtubules onto overlying actin filaments consistent with the relatively faster detachment of kinesin-2 at near-maximal forces. Thus, in addition to relative force, switching probability at filament intersections is determined by the dynamics of motor-filament interaction, such as the quick detachment of kinesin-2 under load. This may enable fine-tuning of filament switching in the cell.
Al-Haddad,
Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility.
2001, Pubmed
Al-Haddad,
Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility.
2001,
Pubmed Ali,
Myosin V and Kinesin act as tethers to enhance each others' processivity.
2008,
Pubmed Ali,
Myosin Va maneuvers through actin intersections and diffuses along microtubules.
2007,
Pubmed Aspengren,
New insights into melanosome transport in vertebrate pigment cells.
2009,
Pubmed Bridgman,
Myosin Va movements in normal and dilute-lethal axons provide support for a dual filament motor complex.
1999,
Pubmed Brunnbauer,
Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner.
2010,
Pubmed Caviston,
Microtubule motors at the intersection of trafficking and transport.
2006,
Pubmed Clancy,
A universal pathway for kinesin stepping.
2011,
Pubmed Clemen,
Force-dependent stepping kinetics of myosin-V.
2005,
Pubmed Dixit,
Differential regulation of dynein and kinesin motor proteins by tau.
2008,
Pubmed Gross,
Cargo transport: two motors are sometimes better than one.
2007,
Pubmed Gross,
Interactions and regulation of molecular motors in Xenopus melanophores.
2002,
Pubmed
,
Xenbase Holzbaur,
Coordination of molecular motors: from in vitro assays to intracellular dynamics.
2010,
Pubmed Kad,
Load and Pi control flux through the branched kinetic cycle of myosin V.
2008,
Pubmed Kojima,
Mechanics of single kinesin molecules measured by optical trapping nanometry.
1997,
Pubmed Levi,
Organelle transport along microtubules in Xenopus melanophores: evidence for cooperation between multiple motors.
2006,
Pubmed
,
Xenbase Mallik,
Building complexity: an in vitro study of cytoplasmic dynein with in vivo implications.
2005,
Pubmed Müller,
Tug-of-war as a cooperative mechanism for bidirectional cargo transport by molecular motors.
2008,
Pubmed Muthukrishnan,
The processivity of kinesin-2 motors suggests diminished front-head gating.
2009,
Pubmed Nishiyama,
Chemomechanical coupling of the forward and backward steps of single kinesin molecules.
2002,
Pubmed Rogers,
Myosin cooperates with microtubule motors during organelle transport in melanophores.
1998,
Pubmed
,
Xenbase Rogers,
Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro.
1997,
Pubmed
,
Xenbase Ross,
Kinesin and dynein-dynactin at intersecting microtubules: motor density affects dynein function.
2008,
Pubmed Sakamoto,
Direct observation of processive movement by individual myosin V molecules.
2000,
Pubmed Schnitzer,
Force production by single kinesin motors.
2000,
Pubmed Schroeder,
Motor number controls cargo switching at actin-microtubule intersections in vitro.
2010,
Pubmed Svoboda,
Force and velocity measured for single kinesin molecules.
1994,
Pubmed Trybus,
Myosin V from head to tail.
2008,
Pubmed Tuma,
Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores.
1998,
Pubmed
,
Xenbase Valentine,
To step or not to step? How biochemistry and mechanics influence processivity in Kinesin and Eg5.
2007,
Pubmed Verhey,
Traffic control: regulation of kinesin motors.
2009,
Pubmed Vershinin,
Multiple-motor based transport and its regulation by Tau.
2007,
Pubmed Wu,
Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo.
1998,
Pubmed
