Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Neuron
2013 Jan 23;772:288-98. doi: 10.1016/j.neuron.2012.12.021.
Show Gene links
Show Anatomy links
Subunit interactions during cooperative opening of voltage-gated proton channels.
Qiu F, Rebolledo S, Gonzalez C, Larsson HP.
???displayArticle.abstract???
Voltage-gated proton (Hv1) channels are dimers, where each subunit has a separate permeation pathway. However, opening of the two pathways is highly cooperative. It is unclear how Hv1 channels open their permeation pathways, because Hv1 channels lack a classic pore domain. Using voltage-clamp fluorometry, we here detect two conformational changes reported by a fluorophore attached to the voltage sensor S4 in Hv1 channels. The first is voltage dependent and precedes channel opening, with properties consistent with reporting on independent S4 charge movements in the two subunits. The second is less voltage dependent and closely correlates with channel opening. Mutations that reduce dimerization or alter the intersubunit interface affect both the second conformational change and channel opening. These observations suggest that, following an initial S4 charge movement in the two subunits, there is a second, cooperative conformational change, involving interactions between subunits, that opens both pathways in Hv1 channels.
Berger,
The pore of the voltage-gated proton channel.
2011, Pubmed,
Xenbase
Berger,
The pore of the voltage-gated proton channel.
2011,
Pubmed
,
Xenbase Cha,
Characterizing voltage-dependent conformational changes in the Shaker K+ channel with fluorescence.
1997,
Pubmed
,
Xenbase Cherny,
The voltage-activated hydrogen ion conductance in rat alveolar epithelial cells is determined by the pH gradient.
1995,
Pubmed Decoursey,
Voltage-gated proton channels and other proton transfer pathways.
2003,
Pubmed DeCoursey,
Voltage-gated proton channels find their dream job managing the respiratory burst in phagocytes.
2010,
Pubmed El Chemaly,
VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification.
2010,
Pubmed Fujiwara,
The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H(+) channel Hv1.
2012,
Pubmed Gonzalez,
Strong cooperativity between subunits in voltage-gated proton channels.
2010,
Pubmed Gonzalez,
Molecular mechanism of voltage sensing in voltage-gated proton channels.
2013,
Pubmed
,
Xenbase Hong,
Voltage-sensing domain of voltage-gated proton channel Hv1 shares mechanism of block with pore domains.
2013,
Pubmed
,
Xenbase Hoshi,
Shaker potassium channel gating. I: Transitions near the open state.
1994,
Pubmed
,
Xenbase Iovannisci,
Function of the HVCN1 proton channel in airway epithelia and a naturally occurring mutation, M91T.
2010,
Pubmed Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed Kalia,
Common principles of voltage-dependent gating for Hv and Kv channels.
2013,
Pubmed Koch,
Multimeric nature of voltage-gated proton channels.
2008,
Pubmed
,
Xenbase Koch,
Small-scale molecular motions accomplish glutamate uptake in human glutamate transporters.
2005,
Pubmed
,
Xenbase Koch,
Voltage-independent sodium-binding events reported by the 4B-4C loop in the human glutamate transporter excitatory amino acid transporter 3.
2007,
Pubmed
,
Xenbase Lee,
Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.
2008,
Pubmed Lishko,
Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel.
2010,
Pubmed Liu,
Gated access to the pore of a voltage-dependent K+ channel.
1997,
Pubmed Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed Mannuzzu,
Direct physical measure of conformational rearrangement underlying potassium channel gating.
1996,
Pubmed
,
Xenbase Morgan,
Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis.
2009,
Pubmed Murata,
Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor.
2005,
Pubmed
,
Xenbase Musset,
Aspartate 112 is the selectivity filter of the human voltage-gated proton channel.
2011,
Pubmed Ramsey,
A voltage-gated proton-selective channel lacking the pore domain.
2006,
Pubmed Sakata,
Functionality of the voltage-gated proton channel truncated in S4.
2010,
Pubmed Sasaki,
A voltage sensor-domain protein is a voltage-gated proton channel.
2006,
Pubmed Thomas,
Hydrogen ion currents and intracellular pH in depolarized voltage-clamped snail neurones.
1982,
Pubmed Tombola,
The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor.
2008,
Pubmed
,
Xenbase Tombola,
The opening of the two pores of the Hv1 voltage-gated proton channel is tuned by cooperativity.
2010,
Pubmed
,
Xenbase Wu,
The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke.
2012,
Pubmed Zagotta,
Shaker potassium channel gating. II: Transitions in the activation pathway.
1994,
Pubmed
,
Xenbase
