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Extracts prepared from the eggs of frogs, particularly Xenopus species, have provided critical material for seminal studies of nuclear and chromosome dynamics over several decades. Their usefulness for these types of analyses lies in several important characteristics: stockpiled nuclear components, absence of endogenous DNA, and intact and functioning signaling networks. These factors have allowed detailed molecular analyses of many aspects of chromosome biology, including DNA replication, checkpoint signaling, epigenetic control, and chromosome condensation, cohesion, and segregation. In this introduction, the preparation and application of Xenopus egg extracts for the study of chromosomes and chromatin are described in detail.
Anderson,
Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum.
2007, Pubmed,
Xenbase
Anderson,
Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum.
2007,
Pubmed
,
Xenbase Blow,
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.
1993,
Pubmed
,
Xenbase Blow,
Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.
1987,
Pubmed
,
Xenbase Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Chen,
Nucleus Assembly and Import in Xenopus laevis Egg Extract.
2018,
Pubmed
,
Xenbase Costanzo,
Xenopus cell-free extracts to study the DNA damage response.
2004,
Pubmed
,
Xenbase da Silva,
Chromosome Cohesion and Condensation in Xenopus Egg Extracts.
2019,
Pubmed
,
Xenbase Flores Servin,
Centromere and Kinetochore Assembly in Xenopus laevis Egg Extract.
2018,
Pubmed
,
Xenbase Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase Gillespie,
Scc2 couples replication licensing to sister chromatid cohesion in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase Görlich,
Isolation of a protein that is essential for the first step of nuclear protein import.
1994,
Pubmed
,
Xenbase GURDON,
Adult frogs derived from the nuclei of single somatic cells.
1962,
Pubmed
,
Xenbase GURDON,
Sexually mature individuals of Xenopus laevis from the transplantation of single somatic nuclei.
1958,
Pubmed
,
Xenbase GURDON,
The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles.
1962,
Pubmed
,
Xenbase Guse,
In vitro centromere and kinetochore assembly on defined chromatin templates.
2011,
Pubmed
,
Xenbase Havens,
Docking of a specialized PIP Box onto chromatin-bound PCNA creates a degron for the ubiquitin ligase CRL4Cdt2.
2009,
Pubmed
,
Xenbase Hazel,
Isolation and Demembranation of Xenopus Sperm Nuclei.
2018,
Pubmed
,
Xenbase Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase Kalab,
The ran GTPase regulates mitotic spindle assembly.
1999,
Pubmed
,
Xenbase Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase Lupardus,
Analyzing the ATR-mediated checkpoint using Xenopus egg extracts.
2007,
Pubmed
,
Xenbase Mao,
Analysis of Mitotic Checkpoint Function in Xenopus Egg Extracts.
2018,
Pubmed
,
Xenbase McGarry,
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
1998,
Pubmed
,
Xenbase Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Mühlhäusser,
An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown.
2007,
Pubmed
,
Xenbase Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Rankin,
Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.
2005,
Pubmed
,
Xenbase Shechter,
Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
2009,
Pubmed
,
Xenbase Sumara,
Characterization of vertebrate cohesin complexes and their regulation in prophase.
2000,
Pubmed
,
Xenbase Takahashi,
Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex.
2004,
Pubmed
,
Xenbase Walter,
Regulation of replicon size in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase Walter,
Regulated chromosomal DNA replication in the absence of a nucleus.
1998,
Pubmed
,
Xenbase Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase Walter,
Evidence for sequential action of cdc7 and cdk2 protein kinases during initiation of DNA replication in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Wang,
Chromatin Characterization in Xenopus laevis Cell-Free Egg Extracts and Embryos.
2019,
Pubmed
,
Xenbase Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
