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High-throughput sequencing methods have created exciting opportunities to explore the regulatory landscape of the entire genome. Here we introduce methods to characterize the genomic locations of bound proteins, open chromatin, and sites of DNA-DNA contact in Xenopus embryos. These methods include chromatin immunoprecipitation followed by sequencing (ChIP-seq), a combination of DNase I digestion and sequencing (DNase-seq), the assay for transposase-accessible chromatin and sequencing (ATAC-seq), and the use of proximity-based DNA ligation followed by sequencing (Hi-C).
Akkers,
A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos.
2009, Pubmed,
Xenbase
Akkers,
A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos.
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Pubmed
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Xenbase Boyle,
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Pubmed Briggs,
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Pubmed
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Xenbase Bright,
Assay for Transposase-Accessible Chromatin-Sequencing Using Xenopus Embryos.
2019,
Pubmed
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Xenbase Buenrostro,
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Pubmed Chen,
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Xenbase Cho,
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Xenbase Cusanovich,
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Xenbase Gentsch,
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Xenbase Hontelez,
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Xenbase Quigley,
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Xenbase
