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Apical constriction refers to the active, actomyosin-driven process that reduces apical cell surface area in epithelial cells. Apical constriction is utilized in epithelial morphogenesis during embryonic development in multiple contexts, such as gastrulation, neural tube closure, and organogenesis. Defects in apical constriction can result in congenital birth defects, yet our understanding of the molecular control of apical constriction is relatively limited. To uncover new genetic regulators of apical constriction and gain mechanistic insight into the cell biology of this process, we need reliable assay systems that allow real-time observation and quantification of apical constriction as it occurs and permit gain- and loss-of-function analyses to explore gene function and interaction during apical constriction. In this chapter, we describe using the early Xenopus embryo as an assay system to investigate molecular mechanisms involved in apical constriction during both gastrulation and neurulation.
Aigouy,
Segmentation and Quantitative Analysis of Epithelial Tissues.
2016, Pubmed
Aigouy,
Segmentation and Quantitative Analysis of Epithelial Tissues.
2016,
Pubmed Blum,
Morpholinos: Antisense and Sensibility.
2015,
Pubmed
,
Xenbase Booth,
A dynamic microtubule cytoskeleton directs medial actomyosin function during tube formation.
2014,
Pubmed Centers for Disease Control and Prevention (CDC),
Spina bifida and anencephaly before and after folic acid mandate--United States, 1995-1996 and 1999-2000.
2004,
Pubmed Chang,
Animal Cap Assay for TGF-β Signaling.
2016,
Pubmed
,
Xenbase Chen,
Genetic and functional analysis of SHROOM1-4 in a Chinese neural tube defect cohort.
2018,
Pubmed Choi,
The involvement of lethal giant larvae and Wnt signaling in bottle cell formation in Xenopus embryos.
2009,
Pubmed
,
Xenbase Christodoulou,
Cell-Autonomous Ca(2+) Flashes Elicit Pulsed Contractions of an Apical Actin Network to Drive Apical Constriction during Neural Tube Closure.
2015,
Pubmed
,
Xenbase Chu,
Lulu regulates Shroom-induced apical constriction during neural tube closure.
2013,
Pubmed
,
Xenbase Claret,
PI(4,5)P2 produced by the PI4P5K SKTL controls apical size by tethering PAR-3 in Drosophila epithelial cells.
2014,
Pubmed Das,
The interaction between Shroom3 and Rho-kinase is required for neural tube morphogenesis in mice.
2014,
Pubmed David,
The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila.
2010,
Pubmed David,
Bazooka inhibits aPKC to limit antagonism of actomyosin networks during amnioserosa apical constriction.
2013,
Pubmed Deshwar,
A homozygous pathogenic variant in SHROOM3 associated with anencephaly and cleft lip and palate.
2020,
Pubmed Edwards,
GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila.
1997,
Pubmed Eisen,
Controlling morpholino experiments: don't stop making antisense.
2008,
Pubmed
,
Xenbase Etournay,
TissueMiner: A multiscale analysis toolkit to quantify how cellular processes create tissue dynamics.
2016,
Pubmed Farrell,
SEGGA: a toolset for rapid automated analysis of epithelial cell polarity and dynamics.
2017,
Pubmed Fiuza,
A Nodal/Eph signalling relay drives the transition from apical constriction to apico-basal shortening in ascidian endoderm invagination.
2020,
Pubmed Fox,
Abelson kinase (Abl) and RhoGEF2 regulate actin organization during cell constriction in Drosophila.
2007,
Pubmed Haigo,
Shroom induces apical constriction and is required for hingepoint formation during neural tube closure.
2003,
Pubmed
,
Xenbase Itoh,
GEF-H1 functions in apical constriction and cell intercalations and is essential for vertebrate neural tube closure.
2014,
Pubmed
,
Xenbase James-Zorn,
Navigating Xenbase: An Integrated Xenopus Genomics and Gene Expression Database.
2018,
Pubmed
,
Xenbase Ji,
EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
2014,
Pubmed
,
Xenbase Kieserman,
High-magnification in vivo imaging of Xenopus embryos for cell and developmental biology.
2010,
Pubmed
,
Xenbase Kim,
Investigating morphogenesis in Xenopus embryos: imaging strategies, processing, and analysis.
2013,
Pubmed
,
Xenbase Ko,
Microtubules promote intercellular contractile force transmission during tissue folding.
2019,
Pubmed Kölsch,
Control of Drosophila gastrulation by apical localization of adherens junctions and RhoGEF2.
2007,
Pubmed Kurth,
Bottle cell formation in relation to mesodermal patterning in the Xenopus embryo.
2000,
Pubmed
,
Xenbase Lang,
p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis.
2014,
Pubmed Lee,
The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets.
2009,
Pubmed
,
Xenbase Lee,
Shroom family proteins regulate gamma-tubulin distribution and microtubule architecture during epithelial cell shape change.
2007,
Pubmed
,
Xenbase Lee,
Actomyosin contractility and microtubules drive apical constriction in Xenopus bottle cells.
2007,
Pubmed
,
Xenbase Lee,
Endocytosis is required for efficient apical constriction during Xenopus gastrulation.
2010,
Pubmed
,
Xenbase Lemay,
Loss-of-function de novo mutations play an important role in severe human neural tube defects.
2015,
Pubmed Manning,
The Fog signaling pathway: insights into signaling in morphogenesis.
2014,
Pubmed Marivin,
GPCR-independent activation of G proteins promotes apical cell constriction in vivo.
2019,
Pubmed
,
Xenbase Marston,
MRCK-1 Drives Apical Constriction in C. elegans by Linking Developmental Patterning to Force Generation.
2016,
Pubmed Martin,
Apical constriction: themes and variations on a cellular mechanism driving morphogenesis.
2014,
Pubmed Martin,
Pulsed contractions of an actin-myosin network drive apical constriction.
2009,
Pubmed McGreevy,
Shroom3 functions downstream of planar cell polarity to regulate myosin II distribution and cellular organization during neural tube closure.
2015,
Pubmed Miao,
Cell ratcheting through the Sbf RabGEF directs force balancing and stepped apical constriction.
2019,
Pubmed Mulinari,
DRhoGEF2 and diaphanous regulate contractile force during segmental groove morphogenesis in the Drosophila embryo.
2008,
Pubmed Nenni,
Xenbase: Facilitating the Use of Xenopus to Model Human Disease.
2019,
Pubmed
,
Xenbase Ossipova,
Vangl2 cooperates with Rab11 and Myosin V to regulate apical constriction during vertebrate gastrulation.
2015,
Pubmed
,
Xenbase Plageman,
A Trio-RhoA-Shroom3 pathway is required for apical constriction and epithelial invagination.
2011,
Pubmed Popov,
The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation.
2018,
Pubmed
,
Xenbase Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed Sawyer,
Apical constriction: a cell shape change that can drive morphogenesis.
2010,
Pubmed
,
Xenbase Sulistomo,
Formin homology 2 domain-containing 3 (Fhod3) controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction.
2019,
Pubmed Sullivan-Brown,
Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation.
2016,
Pubmed
,
Xenbase Suzuki,
Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure.
2017,
Pubmed
,
Xenbase Suzuki,
MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.
2010,
Pubmed
,
Xenbase Wallingford,
Xenopus.
2010,
Pubmed
,
Xenbase Wallingford,
Preparation of fixed Xenopus embryos for confocal imaging.
2010,
Pubmed
,
Xenbase
