Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The stem-loop binding protein (SLBP) binds to the 3' end of histone mRNA and participates in 3'-processing of the newly synthesized transcripts, which protects them from degradation, and probably also promotes their translation. In proliferating cells, translation of SLBP mRNA begins at G1/S and the protein is degraded following DNA replication. These post-transcriptional mechanisms closely couple SLBP expression to S-phase of the cell cycle, and play a key role in restricting synthesis of replication-dependent histones to S-phase. In contrast to somatic cells, replication-dependent histone mRNAs accumulate and are translated independently of DNA replication in oocytes and early embryos. We report here that SLBP expression and activity also differ in mouse oocytes and early embryos compared with somatic cells. SLBP is present in oocytes that are arrested at prophase of G2/M, where it is concentrated in the nucleus. Upon entry into M-phase of meiotic maturation, SLBP begins to accumulate rapidly, reaching a very high level in mature oocytes arrested at metaphase II. Following fertilization, SLBP remains abundant in the nucleus and the cytoplasm throughout the first cell cycle, including both G1 and G2 phases. It declines during the second and third cell cycles, reaching a relatively low level by the late 4-cell stage. SLBP can bind the histone mRNA-stem-loop at all stages of the cell cycle in oocytes and early embryos, and it is the only stem-loop binding activity detectable in these cells. We also report that SLBP becomes phosphorylated rapidly following entry into M-phase of meiotic maturation through a mechanism that is sensitive to roscovitine, an inhibitor of cyclin-dependent kinases. SLBP is rapidly dephosphorylated following fertilization or parthenogenetic activation, and becomes newly phosphorylated at M-phase of mitosis. Phosphorylation does not affect its stem-loop binding activity. These results establish that, in contrast to Xenopus, mouse oocytes and embryos contain a single SLBP. Expression of SLBP is uncoupled from S-phase in oocytes and early embryos, which indicates that the mechanisms that impose cell-cycle-regulated expression of SLBP in somatic cells do not operate in oocytes or during the first embryonic cell cycle. This distinctive pattern of SLBP expression may be required for accumulation of histone proteins required for sperm chromatin remodelling and assembly of newly synthesized embryonic DNA into chromatin.
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999, Pubmed,
Xenbase
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999,
Pubmed
,
Xenbase Barkoff,
Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation.
2000,
Pubmed
,
Xenbase Bolton,
The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo.
1984,
Pubmed Choi,
Activation of p34cdc2 protein kinase activity in meiotic and mitotic cell cycles in mouse oocytes and embryos.
1991,
Pubmed Clarke,
Developmental regulation of chromatin composition during mouse embryogenesis: somatic histone H1 is first detectable at the 4-cell stage.
1992,
Pubmed Clarke,
Chromatin modifications during oogenesis in the mouse: removal of somatic subtypes of histone H1 from oocyte chromatin occurs post-natally through a post-transcriptional mechanism.
1997,
Pubmed Culp,
c-mos and cdc2 cooperate in the translational activation of fibroblast growth factor receptor-1 during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase Deng,
A specific inhibitor of p34(cdc2)/cyclin B suppresses fertilization-induced calcium oscillations in mouse eggs.
2000,
Pubmed Dominski,
Stem-loop binding protein facilitates 3'-end formation by stabilizing U7 snRNP binding to histone pre-mRNA.
1999,
Pubmed Dominski,
A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing.
2002,
Pubmed
,
Xenbase Dominski,
Mutations in the RNA binding domain of stem-loop binding protein define separable requirements for RNA binding and for histone pre-mRNA processing.
2001,
Pubmed
,
Xenbase Eliassen,
Role for a YY1-binding element in replication-dependent mouse histone gene expression.
1998,
Pubmed Gallie,
The histone 3'-terminal stem-loop is necessary for translation in Chinese hamster ovary cells.
1996,
Pubmed Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed Gick,
Heat-labile regulatory factor is required for 3' processing of histone precursor mRNAs.
1987,
Pubmed Giebelhaus,
Changes in the quantity of histone and actin messenger RNA during the development of preimplantation mouse embryos.
1983,
Pubmed Graves,
Quantitative and qualitative changes in histone gene expression during early mouse embryo development.
1985,
Pubmed Gray,
Control of translation initiation in animals.
1998,
Pubmed Hanson,
Efficient extraction and partial purification of the polyribosome-associated stem-loop binding protein bound to the 3' end of histone mRNA.
1996,
Pubmed Harris,
Regulation of histone mRNA in the unperturbed cell cycle: evidence suggesting control at two posttranscriptional steps.
1991,
Pubmed Howlett,
Sequence and regulation of morphological and molecular events during the first cell cycle of mouse embryogenesis.
1985,
Pubmed Huarte,
Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA.
1987,
Pubmed Ingledue,
Dual role for the RNA-binding domain of Xenopus laevis SLBP1 in histone pre-mRNA processing.
2000,
Pubmed
,
Xenbase Kodama,
The stem-loop binding protein CDL-1 is required for chromosome condensation, progression of cell death and morphogenesis in Caenorhabditis elegans.
2002,
Pubmed Lawitts,
Culture of preimplantation embryos.
1993,
Pubmed Martin,
The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein.
1997,
Pubmed Masui,
Oocyte maturation.
1979,
Pubmed Meijer,
Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5.
1997,
Pubmed
,
Xenbase Mohamed,
High-mobility group proteins 14 and 17 maintain the timing of early embryonic development in the mouse.
2001,
Pubmed Müller,
Assembly of U7 small nuclear ribonucleoprotein particle and histone RNA 3' processing in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Oh,
Timely translation during the mouse oocyte-to-embryo transition.
2000,
Pubmed
,
Xenbase O'Keefe,
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
1989,
Pubmed Pandey,
Point mutations in the stem-loop at the 3' end of mouse histone mRNA reduce expression by reducing the efficiency of 3' end formation.
1994,
Pubmed Paules,
Mouse Mos protooncogene product is present and functions during oogenesis.
1989,
Pubmed
,
Xenbase Paynton,
Changes in state of adenylation and time course of degradation of maternal mRNAs during oocyte maturation and early embryonic development in the mouse.
1988,
Pubmed Pettitt,
The Caenorhabditis elegans histone hairpin-binding protein is required for core histone gene expression and is essential for embryonic and postembryonic cell division.
2002,
Pubmed Polanski,
Cyclin synthesis controls the progression of meiotic maturation in mouse oocytes.
1998,
Pubmed Rempel,
Maternal Xenopus Cdk2-cyclin E complexes function during meiotic and early embryonic cell cycles that lack a G1 phase.
1995,
Pubmed
,
Xenbase Sànchez,
The stem-loop binding protein is required for efficient translation of histone mRNA in vivo and in vitro.
2002,
Pubmed
,
Xenbase Smith,
Analysis of the third and fourth cell cycles of mouse early development.
1986,
Pubmed Stauber,
A signal regulating mouse histone H4 mRNA levels in a mammalian cell cycle mutant and sequences controlling RNA 3' processing are both contained within the same 80-bp fragment.
1986,
Pubmed Stauber,
3' processing of pre-mRNA plays a major role in proliferation-dependent regulation of histone gene expression.
1988,
Pubmed Stutz,
Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes.
1998,
Pubmed Stutz,
In vivo antisense oligodeoxynucleotide mapping reveals masked regulatory elements in an mRNA dormant in mouse oocytes.
1997,
Pubmed Sullivan,
Drosophila stem loop binding protein coordinates accumulation of mature histone mRNA with cell cycle progression.
2001,
Pubmed
,
Xenbase Sun,
The histone mRNA 3' end is required for localization of histone mRNA to polyribosomes.
1992,
Pubmed Tay,
The control of cyclin B1 mRNA translation during mouse oocyte maturation.
2000,
Pubmed Wang,
The protein that binds the 3' end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing.
1996,
Pubmed Wang,
Two Xenopus proteins that bind the 3' end of histone mRNA: implications for translational control of histone synthesis during oogenesis.
1999,
Pubmed
,
Xenbase Wassarman,
Program of early development in the mammal: synthesis and intracellular migration of histone H4 during oogenesis in the mouse.
1981,
Pubmed Whitfield,
Stem-loop binding protein, the protein that binds the 3' end of histone mRNA, is cell cycle regulated by both translational and posttranslational mechanisms.
2000,
Pubmed Wiekowski,
Changes in histone synthesis and modification at the beginning of mouse development correlate with the establishment of chromatin mediated repression of transcription.
1997,
Pubmed Williams,
The sequence of the stem and flanking sequences at the 3' end of histone mRNA are critical determinants for the binding of the stem-loop binding protein.
1995,
Pubmed
