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Phosphorylation-dependent and phosphorylation-independent modes of modulation of shaker family voltage-gated potassium channels by SRC family protein tyrosine kinases.
Nitabach MN, Llamas DA, Thompson IJ, Collins KA, Holmes TC.
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Modulation of voltage-gated potassium (Kv) channels by protein phosphorylation plays an essential role in the regulation of the membrane properties of cells. Protein-protein binding domains, such as Src homology 3 (SH3) domains, direct ion channel modulation by coupling the channels with intracellular signaling enzymes. The conventional view is that protein kinase binding to ion channels leads to modulation by bringing the channel substrate into physical proximity to the enzyme, thereby fostering covalent modification of the channel. The SH3 domain binding-dependent functional suppression of Kv1.5 currents by Src family protein tyrosine kinases (PTKs) is considered a canonical example of this type of mechanism. In the present study we address whether the SH3-dependent binding of Src family PTKs to Shaker family Kvs mediates modulatory events that are independent of and/or dependent on Src-catalyzed tyrosine phosphorylation of the channel. We find that Src binding and tyrosine phosphorylation are each able to modulate Kv1 family macroscopic channel currents independently. SH3-dependent binding of Src leads to the suppression of both Kv1.5 and Kv1.4 (modified to contain proline-rich SH3 domain binding sites) macroscopic currents even in the absence of Src-catalyzed tyrosine phosphorylation, whereas binding-independent tyrosine phosphorylation by Src leads to the suppression of Kv1.5 macroscopic currents and the modulation of Kv1.4 inactivation kinetics.
Bowlby,
Modulation of the Kv1.3 potassium channel by receptor tyrosine kinases.
1997, Pubmed
Bowlby,
Modulation of the Kv1.3 potassium channel by receptor tyrosine kinases.
1997,
Pubmed Cayabyab,
Suppression of the rat microglia Kv1.3 current by src-family tyrosine kinases and oxygen/glucose deprivation.
2000,
Pubmed Chung,
Protein kinase activity closely associated with a reconstituted calcium-activated potassium channel.
1991,
Pubmed Cook,
Two adaptor proteins differentially modulate the phosphorylation and biophysics of Kv1.3 ion channel by SRC kinase.
2002,
Pubmed Ebina,
Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity.
1987,
Pubmed Fadool,
Tyrosine phosphorylation modulates current amplitude and kinetics of a neuronal voltage-gated potassium channel.
1997,
Pubmed Fadool,
Modulation of olfactory bulb neuron potassium current by tyrosine phosphorylation.
1998,
Pubmed Feng,
Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.
1995,
Pubmed Goldin,
Maintenance of Xenopus laevis and oocyte injection.
1992,
Pubmed
,
Xenbase He,
Identification of critical residues controlling G protein-gated inwardly rectifying K(+) channel activity through interactions with the beta gamma subunits of G proteins.
2002,
Pubmed Holmes,
Tyrosine phosphorylation of the Kv1.3 potassium channel.
1996,
Pubmed Holmes,
Expression of voltage-gated potassium channels decreases cellular protein tyrosine phosphorylation.
1997,
Pubmed Holmes,
Association of Src tyrosine kinase with a human potassium channel mediated by SH3 domain.
1996,
Pubmed Holz,
GTP-binding proteins mediate transmitter inhibition of voltage-dependent calcium channels.
,
Pubmed Huang,
Tyrosine kinase-dependent suppression of a potassium channel by the G protein-coupled m1 muscarinic acetylcholine receptor.
1993,
Pubmed
,
Xenbase Hubbard,
Src autoinhibition: let us count the ways.
1999,
Pubmed Jing,
Fast inactivation of a brain K+ channel composed of Kv1.1 and Kvbeta1.1 subunits modulated by G protein beta gamma subunits.
1999,
Pubmed
,
Xenbase Johnson,
Active and inactive protein kinases: structural basis for regulation.
1996,
Pubmed Kamps,
Neither arginine nor histidine can carry out the function of lysine-295 in the ATP-binding site of p60src.
1986,
Pubmed Kanemitsu,
Tyrosine phosphorylation of connexin 43 by v-Src is mediated by SH2 and SH3 domain interactions.
1997,
Pubmed Li,
Surface expression of Kv1 channels is governed by a C-terminal motif.
2000,
Pubmed Logothetis,
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.
,
Pubmed Ma,
Persistent sodium currents through brain sodium channels induced by G protein betagamma subunits.
1997,
Pubmed Mark,
G-protein mediated gating of inward-rectifier K+ channels.
2000,
Pubmed
,
Xenbase Maximov,
Association of neuronal calcium channels with modular adaptor proteins.
1999,
Pubmed Molokanova,
Noncatalytic inhibition of cyclic nucleotide-gated channels by tyrosine kinase induced by genistein.
1999,
Pubmed
,
Xenbase Murray,
Modulation of an inactivating human cardiac K+ channel by protein kinase C.
1994,
Pubmed
,
Xenbase Nitabach,
A mechanism for combinatorial regulation of electrical activity: Potassium channel subunits capable of functioning as Src homology 3-dependent adaptors.
2001,
Pubmed
,
Xenbase Okada,
Deletion of the SH3 domain of Src interferes with regulation by the phosphorylated carboxyl-terminal tyrosine.
1993,
Pubmed Rickles,
Identification of Src, Fyn, Lyn, PI3K and Abl SH3 domain ligands using phage display libraries.
1994,
Pubmed Roeper,
Frequency-dependent inactivation of mammalian A-type K+ channel KV1.4 regulated by Ca2+/calmodulin-dependent protein kinase.
1997,
Pubmed Santoro,
Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels.
1997,
Pubmed Seidel-Dugan,
Effects of SH2 and SH3 deletions on the functional activities of wild-type and transforming variants of c-Src.
1992,
Pubmed Snyder,
A mutation at the ATP-binding site of pp60v-src abolishes kinase activity, transformation, and tumorigenicity.
1985,
Pubmed Sobko,
Constitutive activation of delayed-rectifier potassium channels by a src family tyrosine kinase in Schwann cells.
1998,
Pubmed Stein,
Combined deficiencies of Src, Fyn, and Yes tyrosine kinases in mutant mice.
1994,
Pubmed Szabò,
Tyrosine phosphorylation-dependent suppression of a voltage-gated K+ channel in T lymphocytes upon Fas stimulation.
1996,
Pubmed Takimoto,
Altered K+ channel subunit composition following hormone induction of Kv1.5 gene expression.
1996,
Pubmed Tong,
Tyrosine decaging leads to substantial membrane trafficking during modulation of an inward rectifier potassium channel.
2001,
Pubmed
,
Xenbase Xu,
Crystal structures of c-Src reveal features of its autoinhibitory mechanism.
1999,
Pubmed Yu,
Structural basis for the binding of proline-rich peptides to SH3 domains.
1994,
Pubmed Zamponi,
Modulation of voltage-dependent calcium channels by G proteins.
1998,
Pubmed
