Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2001 Apr 24;989:5288-93. doi: 10.1073/pnas.081089198.
Show Gene links
Show Anatomy links
Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X2-green fluorescent protein receptors.
Khakh BS, Smith WB, Chiu CS, Ju D, Davidson N, Lester HA.
???displayArticle.abstract???
ATP-gated P2X(2) receptors are widely expressed in neurons, but the cellular effects of receptor activation are unclear. We engineered functional green fluorescent protein (GFP)-tagged P2X(2) receptors and expressed them in embryonic hippocampal neurons, and report an approach to determining functional and total receptor pool sizes in living cells. ATP application to dendrites caused receptor redistribution and the formation of varicose hot spots of higher P2X(2)-GFP receptor density. Redistribution in dendrites was accompanied by an activation-dependent enhancement of the ATP-evoked current. Substate-specific mutant T18A P2X(2)-GFP receptors showed no redistribution or activation-dependent enhancement of the ATP-evoked current. Thus fluorescent P2X(2)-GFP receptors function normally, can be quantified, and reveal the dynamics of P2X(2) receptor distribution on the seconds time scale.
Boué-Grabot,
A protein kinase C site highly conserved in P2X subunits controls the desensitization kinetics of P2X(2) ATP-gated channels.
2000, Pubmed,
Xenbase
Boué-Grabot,
A protein kinase C site highly conserved in P2X subunits controls the desensitization kinetics of P2X(2) ATP-gated channels.
2000,
Pubmed
,
Xenbase Brake,
New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor.
1994,
Pubmed
,
Xenbase Carroll,
Dynamin-dependent endocytosis of ionotropic glutamate receptors.
1999,
Pubmed Carroll,
Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures.
1999,
Pubmed Chessell,
Properties of the pore-forming P2X7 purinoceptor in mouse NTW8 microglial cells.
1997,
Pubmed Chiu,
Single-molecule measurements calibrate green fluorescent protein surface densities on transparent beads for use with 'knock-in' animals and other expression systems.
2001,
Pubmed Collo,
Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels.
1996,
Pubmed Cook,
A memory for extracellular Ca2+ by speeding recovery of P2X receptors from desensitization.
1998,
Pubmed Ding,
Single channel properties of P2X2 purinoceptors.
1999,
Pubmed
,
Xenbase Emery,
Ultrastructural damage and neuritic beading in cold-stressed spinal neurons with comparisons to NMDA and A23187 toxicity.
1995,
Pubmed Gentet,
Direct measurement of specific membrane capacitance in neurons.
2000,
Pubmed Grabner,
Tagging with green fluorescent protein reveals a distinct subcellular distribution of L-type and non-L-type Ca2+ channels expressed in dysgenic myotubes.
1998,
Pubmed Gu,
Expression of P2X(7) purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X(7) receptors.
2000,
Pubmed Jiang,
Identification of amino acid residues contributing to the ATP-binding site of a purinergic P2X receptor.
2000,
Pubmed Khakh,
Dynamic selectivity filters in ion channels.
1999,
Pubmed Khakh,
Neuronal P2X transmitter-gated cation channels change their ion selectivity in seconds.
1999,
Pubmed Khakh,
State-dependent cross-inhibition between transmitter-gated cation channels.
2000,
Pubmed
,
Xenbase Khakh,
International union of pharmacology. XXIV. Current status of the nomenclature and properties of P2X receptors and their subunits.
2001,
Pubmed Khakh,
Molecular physiology of P2X receptors and ATP signalling at synapses.
2001,
Pubmed Khakh,
Allosteric control of gating and kinetics at P2X(4) receptor channels.
1999,
Pubmed
,
Xenbase Kidd,
Localization of P2X purinoceptor transcripts in the rat nervous system.
1995,
Pubmed Lewis,
Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons.
1995,
Pubmed Li,
The distribution of P2X receptor clusters on individual neurons in sympathetic ganglia and their redistribution on agonist activation.
2000,
Pubmed Lissin,
Rapid, activation-induced redistribution of ionotropic glutamate receptors in cultured hippocampal neurons.
1999,
Pubmed Pankratov,
A purinergic component of the excitatory postsynaptic current mediated by P2X receptors in the CA1 neurons of the rat hippocampus.
1998,
Pubmed Rubio,
Distinct Localization of P2X receptors at excitatory postsynaptic specializations.
2001,
Pubmed Shi,
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
1999,
Pubmed Surprenant,
Functional properties of heteromeric P2X(1/5) receptors expressed in HEK cells and excitatory junction potentials in guinea-pig submucosal arterioles.
2000,
Pubmed Virginio,
Pore dilation of neuronal P2X receptor channels.
1999,
Pubmed Virginio,
Kinetics of cell lysis, dye uptake and permeability changes in cells expressing the rat P2X7 receptor.
1999,
Pubmed Wong,
Single channel properties of P2X ATP receptors in outside-out patches from rat hippocampal granule cells.
2000,
Pubmed Zhou,
P2X purinoceptors in cultured myenteric neurons of guinea-pig small intestine.
1996,
Pubmed
