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Confluent high-density cell cultures of A6 cells derived from adult male Xenopus kidney exhibit spontaneous dome-formation at 1 g. To determine whether this morphogenetic property is altered by gravity, we used a three-dimensional (3D) clinostat to subject the cells to simulated microgravity, and a centrifuge to subject them to hypergravity. We used the generation orbit control method as the new rotation control system of the 3D-clinostat, not the random method. The growth of A6 cells was significantly enhanced by hypergravity, but significantly reduced by simulated microgravity. Dome formation by A6 cells at high confluence was inhibited under simulated microgravity conditions, whereas hypergravity promoted dome formation and induced tubule morphogenesis, compared to the control at 1 g. These results indicated that changes in gravity influence the morphogenetic properties of A6 cells, such as dome formation and tubule morphogenesis. When dome formation by A6 cells at high confluence was induced spontaneously in the control 1 g culture, the gene expression of the HGF family of pleiotropic factors, such as HGF-like protein (HLP) and growth factor-Livertine (GF-l.ivertine), an epithelial serine protease of channel activating protease 1 (CAP1), and Na+, K+-adenosine triphosphatase (ATPase), increased. Simulated microgravity increased the gene expression of activin A and reduced the gene expression of HLP, GF-Livertine, CAP1, and Na+, K+-ATPase. Hypergravity, on the other hand, decreased the gene expression of activin A and increased the gene expression of HLP, GF-Livertine, CAP1, and Na+, K+-ATPase. These results suggest that the effects of gravitational changes on expression of the HGF family member gene, CAP1, and Na+, K+-ATPase gene may be important for the cell growth, tubule morphogenesis, and dome formation of A6 cells in altered
Abaza,
Effects of ouabain on the function and structure of a cell line (MDCK) derived from canine kidney. I. Light microscopic observations of monolayer growth.
1974, Pubmed
Abaza,
Effects of ouabain on the function and structure of a cell line (MDCK) derived from canine kidney. I. Light microscopic observations of monolayer growth.
1974,
Pubmed Aberger,
The Xenopus homologue of hepatocyte growth factor-like protein is specifically expressed in the presumptive neural plate during gastrulation.
1996,
Pubmed
,
Xenbase Amsler,
Development of Na+-dependent hexose transport in a cultured line of porcine kidney cells.
1982,
Pubmed Asashima,
Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor).
1990,
Pubmed
,
Xenbase Bellusci,
Creation of an hepatocyte growth factor/scatter factor autocrine loop in carcinoma cells induces invasive properties associated with increased tumorigenicity.
1994,
Pubmed Bullock,
The phylogenetic distribution of electroreception: evidence for convergent evolution of a primitive vertebrate sense modality.
1983,
Pubmed Cereijido,
Structural and functional membrane polarity in cultured monolayers of MDCK cells.
1980,
Pubmed Cohen-Luria,
PGE2 inhibits Na-K-ATPase activity and ouabain binding in MDCK cells.
1993,
Pubmed Fantini,
Spontaneous and induced dome formation by two clonal cell populations derived from a human adenocarcinoma cell line, HT29.
1986,
Pubmed Furue,
Effects of hepatocyte growth factor (HGF) and activin A on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture.
1999,
Pubmed Garty,
Epithelial sodium channels: function, structure, and regulation.
1997,
Pubmed Glynn,
The sodium pump.
1975,
Pubmed Gómez-Lechón,
Cell cycle progression proteins (cyclins), oncogene expression, and signal transduction during the proliferative response of human hepatocytes to hepatocyte growth factor.
1996,
Pubmed Grant,
Arginine vasopressin stimulates net fluid secretion in a polarized subculture of cyst-forming MDCK cells.
1991,
Pubmed Hardman,
Localization of extracellular matrix components in developing mouse salivary glands by confocal microscopy.
1992,
Pubmed Horie,
Mediation of renal cyst formation by hepatocyte growth factor.
1994,
Pubmed Hoson,
Evaluation of the three-dimensional clinostat as a simulator of weightlessness.
1997,
Pubmed Ishibashi,
Hepatocyte growth factor is a paracrine factor for renal epithelial cells: stimulation of DNA synthesis and NA,K-ATPase activity.
1992,
Pubmed Kaneko,
Activation of Na+/H+ exchanger by hepatocyte growth factor in hepatocytes.
1995,
Pubmed Khaoustov,
Induction of three-dimensional assembly of human liver cells by simulated microgravity.
1999,
Pubmed Kirkland,
Dome formation by a human colonic adenocarcinoma cell line (HCA-7).
1985,
Pubmed Klebe,
Cyclic-AMP deficient MDCK cells form tubules.
1995,
Pubmed Kumei,
Reduction of G1 phase duration and enhancement of c-myc gene expression in HeLa cells at hypergravity.
1989,
Pubmed Lamb,
Autoradiographic localisation of [3H]ouabain bound to cultured epithelial cell monolayers of MDCK cells.
1981,
Pubmed Le Bivic,
HT-29 cells are an in vitro model for the generation of cell polarity in epithelia during embryonic differentiation.
1988,
Pubmed Leighton,
Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney.
1969,
Pubmed Lever,
Inducers of mammalian cell differentiation stimulate dome formation in a differentiated kidney epithelial cell line (MDCK).
1979,
Pubmed Levrat,
Differentiation of the human pancreatic adenocarcinoma cell line (Capan-1) in culture and co-culture with fibroblasts dome formation.
1988,
Pubmed Louvard,
Apical membrane aminopeptidase appears at site of cell-cell contact in cultured kidney epithelial cells.
1980,
Pubmed Matsumoto,
Roles of HGF as a pleiotropic factor in organ regeneration.
1993,
Pubmed Michalopoulos,
Control of hepatocyte replication by two serum factors.
1984,
Pubmed Moberly,
A monoclonal antibody that recognizes a basolateral membrane protein in A6 epithelial cells.
1988,
Pubmed Molnar,
Skeletal muscle satellite cells cultured in simulated microgravity.
1997,
Pubmed Montesano,
Identification of a fibroblast-derived epithelial morphogen as hepatocyte growth factor.
1991,
Pubmed Nakamura,
Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats.
1984,
Pubmed Paterson,
The effect of ionophores and related agents on the induction of doming in a rat mammary epithelial cell line.
1985,
Pubmed Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase Pollack,
Na+, K+-ATPase in HeLa cells after prolonged growth in low K+ or ouabain.
1981,
Pubmed Rahmani,
Hepatocyte growth factor activates the AP-1 complex: a comparison between normal and transformed rat hepatocytes.
1999,
Pubmed Rodriguez-Boulan,
Morphogenesis of the polarized epithelial cell phenotype.
1989,
Pubmed Romand,
Qualitative and quantitative observations of spiral ganglion development in the rat.
1985,
Pubmed Rose,
Effects of microgravity on the embryonic pancreas.
1999,
Pubmed Schatz,
Gravity effects on membrane processes.
1994,
Pubmed Stern,
Epithelial scatter factor and development of the chick embryonic axis.
1990,
Pubmed Stoker,
Scatter factor is a fibroblast-derived modulator of epithelial cell mobility.
,
Pubmed Streit,
A role for HGF/SF in neural induction and its expression in Hensen's node during gastrulation.
1995,
Pubmed Takahashi,
Gravimorphogenesis: gravity-regulated formation of the peg in cucumber seedlings.
1997,
Pubmed Thomas,
Stimulation of dome formation in MDCK kidney epithelial cultures by inducers of differentiation: dissociation from effects on transepithelial resistance and cyclic AMP levels.
1982,
Pubmed Uochi,
The Na+,K+-ATPase alpha subunit requires gastrulation in the Xenopus embryo.
1997,
Pubmed
,
Xenbase Vallet,
An epithelial serine protease activates the amiloride-sensitive sodium channel.
1997,
Pubmed
,
Xenbase Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase Wang,
Steps in the morphogenesis of a polarized epithelium. I. Uncoupling the roles of cell-cell and cell-substratum contact in establishing plasma membrane polarity in multicellular epithelial (MDCK) cysts.
1990,
Pubmed Watari,
Dome formation induced by v-H-ras oncogene in a human choriocarcinoma cell line.
1996,
Pubmed Weidner,
Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells.
1990,
Pubmed Yoneyama,
Induction of microvillar hydrolase activities by cell density and exogenous differentiation inducers in an established kidney epithelial cell line (LLC-PK1).
1984,
Pubmed
