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Summary Attributions Wiki
XB-GRNA-23659127
gRNA Name: fbxw7 gRNA1
Synonyms:
Genomic Target: fbxw7

PAM Sequence: GGG
Sequence: 5' AGTGTCTGAGAACACTTGTT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 18/20 19/20
genome
gRNA
 
5' AACAAGTGTTCTCAGACACT 3'
   ||||||||||||||||||||
3' TTGTTCACAAGAGTCTGTGA 5'
 
5' GACAAGTGTTCTTAGACACT 3'
    ||||||||||| |||||||
3' TTGTTCACAAGAGTCTGTGA 5'
 
5' AACAAGTGTTCTTAGACACT 3'
   |||||||||||| |||||||
3' TTGTTCACAAGAGTCTGTGA 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr1S:44603441..44603460 19/20 Sense target fbxw7.S
Chr1L:59005996..59006015 18/20 Sense target fbxw7.L
Chr4L:129809849..129809868 18/20 Sense off-target grm7.L
Chr9_10S:75446869..75446888 17/20 Antisense off-target erbb4.S
Chr6S:70386236..70386255 17/20 Antisense off-target drosha.S
Chr9_10S:1890020..1890039 16/20 Sense off-target ikzf3.S
Chr1S:66481934..66481953 16/20 Antisense off-target grid2.S
Chr4S:59575157..59575176 16/20 Sense off-target adgrl2.S
Chr6L:50624952..50624971 18/20 unknown
Chr6L:95204401..95204420 16/20 unknown
Chr2L:60930528..60930547 16/20 unknown
tropicalis 10.0 Chr1:55548634..55548653 20/20 Sense target fbxw7
Chr2:149456711..149456730 18/20 Antisense off-target LOC108645818
Chr4:90418780..90418799 17/20 Sense off-target XB5735880
Chr7:51557877..51557896 17/20 unknown
Chr1:121957977..121957996 16/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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