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XB-GRNA-23659132
gRNA Name:
stag2 gRNA1
Synonyms: stag2_g1
Genomic Target: stag2
PAM Sequence: GGG
Sequence: 5' GATCCACTTAATGGAGAAGA 3'
Synonyms: stag2_g1
Genomic Target: stag2
PAM Sequence: GGG
Sequence: 5' GATCCACTTAATGGAGAAGA 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 20/20 | 20/20 | 20/20 | ||||
genome
gRNA
|
5' TCTTCTCCATTAAGTGGATC 3' |||||||||||||||||||| 3' AGAAGAGGTAATTCACCTAG 5' |
5' TCTTCTCCATTAAGTGGATC 3' |||||||||||||||||||| 3' AGAAGAGGTAATTCACCTAG 5' |
5' TCTTCTCCATTAAGTGGATC 3' |||||||||||||||||||| 3' AGAAGAGGTAATTCACCTAG 5' |
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Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 10.1 | Chr8L:40127774..40127793 | 20/20 | Sense | target | stag2.L | ||
| Chr8S:53131368..53131387 | 20/20 | Sense | target | stag2.S | |||
| Chr8S:65313370..65313389 | 18/20 | Antisense | off-target | fmr1.S | |||
| Chr3L:92674137..92674156 | 18/20 | Sense | off-target | ube2h.L | |||
| Chr5S:92605945..92605964 | 17/20 | Sense | off-target | dlg1.S | |||
| Chr1L:218587116..218587135 | 17/20 | Sense | off-target | ccbe1.L | |||
| Chr9_10L:116971113..116971132 | 17/20 | Sense | off-target | coro7.L | |||
| Chr1S:189082708..189082727 | 17/20 | Sense | off-target | ccbe1.S | |||
| Chr3L:161078199..161078218 | 16/20 | Sense | off-target | cldn7.L | |||
| Chr1S:8126802..8126821 | 16/20 | Sense | off-target | ctnna2.S | |||
| Chr5L:120073371..120073390 | 16/20 | Antisense | off-target | LOC105947301.L | |||
| Chr8L:118171597..118171616 | 18/20 | unknown | |||||
| Chr9_10L:125999032..125999051 | 18/20 | unknown | |||||
| Chr4S:58166199..58166218 | 17/20 | unknown | |||||
| Chr9_10S:62516154..62516173 | 16/20 | unknown | |||||
| Chr1L:89314799..89314818 | 16/20 | unknown | |||||
| Chr1S:21493897..21493916 | 16/20 | unknown | tropicalis 10.0 | Chr8:48973720..48973739 | 20/20 | Sense | target | stag2 |
| Chr4:125219436..125219455 | 18/20 | Sense | off-target | bsn | |||
| Chr3:87011605..87011624 | 18/20 | Sense | off-target | ube2h | |||
| Chr1:201115356..201115375 | 17/20 | Sense | off-target | ccbe1 | |||
| Chr10:48757060..48757079 | 16/20 | Sense | off-target | hs3st3a1 | |||
| Chr8:118366275..118366294 | 18/20 | unknown | |||||
| Chr2:103467665..103467684 | 17/20 | unknown | |||||
| Chr6:32609723..32609742 | 17/20 | unknown | |||||
| Chr7:96356269..96356288 | 17/20 | unknown | |||||
| Publications | |||||||
| ???morpholino.displaySummary.papersFirst??? |
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020  |
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| ???morpholino.displaySummary.papersRecent??? | |||||||
| ???morpholino.displaySummary.papersAll??? | |||||||
