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Summary Attributions Wiki
XB-GRNA-23659154
gRNA Name: prph2 gRNA1
Synonyms: prph2_L
Genomic Target: prph2

PAM Sequence: AGG
Sequence: 5' AGGAGAGTGAAACTGGCCCA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 20/20 19/20
genome
gRNA
 
5' TGGGCTAGTTTCACTCTCCT 3'
   ||||| ||||||||||||||
3' ACCCGGTCAAAGTGAGAGGA 5'
 
5' TGGGCCAGTTTCACTCTCCT 3'
   ||||||||||||||||||||
3' ACCCGGTCAAAGTGAGAGGA 5'
 
5' TGGGCAAGTTTCACTCTCCT 3'
   ||||| ||||||||||||||
3' ACCCGGTCAAAGTGAGAGGA 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr5L:11047166..11047185 20/20 Sense target prph2.L
Chr5S:17931144..17931163 19/20 Sense target prph2.S
Chr8L:133701585..133701604 18/20 Sense off-target cgn.L
Chr9_10L:97357616..97357635 17/20 Antisense off-target rbfox1.L
Chr1S:7852856..7852875 17/20 Sense off-target ctnna2.S
Chr4L:48963015..48963034 18/20 unknown
Chr5L:43679766..43679785 18/20 unknown
Chr8L:90732892..90732911 17/20 unknown
Chr1S:162822823..162822842 17/20 unknown
Chr2S:115185722..115185741 17/20 unknown
Chr3L:30488968..30488987 17/20 unknown
Chr3S:83135950..83135969 17/20 unknown
Chr3S:96401817..96401836 17/20 unknown
Chr5S:127760696..127760715 17/20 unknown
Chr7S:23583943..23583962 17/20 unknown
Chr9_10S:114067323..114067342 16/20 unknown
Chr2L:21560829..21560848 16/20 unknown
tropicalis 10.0 Chr5:15258363..15258382 19/20 Sense target prph2
Chr4:102403774..102403793 17/20 Antisense off-target dpyd
Chr4:30525759..30525778 17/20 Sense off-target muc2
Chr2:149194998..149195017 17/20 Antisense off-target setdb2
Chr8:110959169..110959188 18/20 unknown
Chr1:171292668..171292687 17/20 unknown
Chr1:29528207..29528226 17/20 unknown
Chr1:96816021..96816040 17/20 unknown
Chr3:110611648..110611667 17/20 unknown
Chr7:103092751..103092770 17/20 unknown
Chr7:87440931..87440950 17/20 unknown
Chr8:69361840..69361859 17/20 unknown
Chr5:83894668..83894687 16/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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